Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells
Phlebotropic flavonoids, including diosmin and its aglycone diosmetin, are natural polyphenols widely used in the prevention and treatment of chronic venous insufficiency (CVI). As oxidative stress plays an important role in the development of pathophysiology of the cardiovascular system, the study...
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MDPI AG
2022-11-01
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| author | Magdalena Wójciak Marcin Feldo Grzegorz Borowski Tomasz Kubrak Bartosz J. Płachno Ireneusz Sowa |
| author_facet | Magdalena Wójciak Marcin Feldo Grzegorz Borowski Tomasz Kubrak Bartosz J. Płachno Ireneusz Sowa |
| author_sort | Magdalena Wójciak |
| collection | DOAJ |
| description | Phlebotropic flavonoids, including diosmin and its aglycone diosmetin, are natural polyphenols widely used in the prevention and treatment of chronic venous insufficiency (CVI). As oxidative stress plays an important role in the development of pathophysiology of the cardiovascular system, the study aimed to investigate the protective effects of diosmin and diosmetin on hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress in endothelial cells. The cells were pretreated with different concentrations of the flavonoid prior to the H<sub>2</sub>O<sub>2</sub> exposure. The cell viability, the level of intracellular reactive oxygen species (ROS), the activity of cellular antioxidant enzymes—including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase GPx—and the malondialdehyde (MDA) level were assessed. It was found that the H<sub>2</sub>O<sub>2</sub>-induced oxidative stress was ameliorated by diosmin/diosmetin in a concentration-dependent manner. The flavonoids restored the activity of cellular antioxidant enzymes and lowered the MDA level upregulated by the H<sub>2</sub>O<sub>2</sub> exposure. These results indicate that diosmin and diosmetin may prevent oxidative stress in endothelial cells; therefore, they may protect against the development and progression of oxidative-stress-related disorders. |
| first_indexed | 2024-03-09T17:39:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:39:59Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-befaabd4d7634c9f8f1bb20cace787e92023-11-24T11:38:49ZengMDPI AGMolecules1420-30492022-11-012723823210.3390/molecules27238232Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial CellsMagdalena Wójciak0Marcin Feldo1Grzegorz Borowski2Tomasz Kubrak3Bartosz J. Płachno4Ireneusz Sowa5Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, PolandDepartment of Vascular Surgery, Medical University of Lublin, Staszica 11 St., 20-081 Lublin, PolandDepartment of Vascular Surgery, Medical University of Lublin, Staszica 11 St., 20-081 Lublin, PolandDepartment of Biochemistry and General Chemistry, Medical College of The University of Rzeszów, 2A Kopisto St., 35-959 Rzeszów, PolandDepartment of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, Gronostajowa 9 St., 30-387 Cracow, PolandDepartment of Analytical Chemistry, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, PolandPhlebotropic flavonoids, including diosmin and its aglycone diosmetin, are natural polyphenols widely used in the prevention and treatment of chronic venous insufficiency (CVI). As oxidative stress plays an important role in the development of pathophysiology of the cardiovascular system, the study aimed to investigate the protective effects of diosmin and diosmetin on hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress in endothelial cells. The cells were pretreated with different concentrations of the flavonoid prior to the H<sub>2</sub>O<sub>2</sub> exposure. The cell viability, the level of intracellular reactive oxygen species (ROS), the activity of cellular antioxidant enzymes—including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase GPx—and the malondialdehyde (MDA) level were assessed. It was found that the H<sub>2</sub>O<sub>2</sub>-induced oxidative stress was ameliorated by diosmin/diosmetin in a concentration-dependent manner. The flavonoids restored the activity of cellular antioxidant enzymes and lowered the MDA level upregulated by the H<sub>2</sub>O<sub>2</sub> exposure. These results indicate that diosmin and diosmetin may prevent oxidative stress in endothelial cells; therefore, they may protect against the development and progression of oxidative-stress-related disorders.https://www.mdpi.com/1420-3049/27/23/8232H<sub>2</sub>O<sub>2</sub> stressdiosmindiosmetinendothelial cellschronic venous insufficiency |
| spellingShingle | Magdalena Wójciak Marcin Feldo Grzegorz Borowski Tomasz Kubrak Bartosz J. Płachno Ireneusz Sowa Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells Molecules H<sub>2</sub>O<sub>2</sub> stress diosmin diosmetin endothelial cells chronic venous insufficiency |
| title | Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells |
| title_full | Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells |
| title_fullStr | Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells |
| title_full_unstemmed | Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells |
| title_short | Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells |
| title_sort | antioxidant potential of diosmin and diosmetin against oxidative stress in endothelial cells |
| topic | H<sub>2</sub>O<sub>2</sub> stress diosmin diosmetin endothelial cells chronic venous insufficiency |
| url | https://www.mdpi.com/1420-3049/27/23/8232 |
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