One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes
Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored. PhC barcodes were decorated with PDA by self-polymerization of DA,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Engineered Regeneration |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666138123000294 |
| _version_ | 1797825619715686400 |
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| author | Dagan Zhang Yuze Wang Junqi Zhao Xueqin Li Yuanyang Zhou Sen Wang |
| author_facet | Dagan Zhang Yuze Wang Junqi Zhao Xueqin Li Yuanyang Zhou Sen Wang |
| author_sort | Dagan Zhang |
| collection | DOAJ |
| description | Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored. PhC barcodes were decorated with PDA by self-polymerization of DA, thus this nanocomposite hybridized PhC barcodes facilitated the adsorption of FITC labelled antibodies and quenched itself photoluminescent, allowing a fast responsive composite platform. In the presence of target analyte, the FITC-labelled detection antibody was released from the surface of PDA decorated microcarrier to specifically bind to the target analyte, thus recovered the photoluminescence. In addition, the PhC microcarrier was enabled to carry out various color barcode for different targets detection though tuning internal periodic structures. Based on these excellent performances of the nanocomposite barcode, this method can not only capture H1N1, H5N1, SARS-CoV-2 simultaneously with rapid, accuracy but also accomplish multiplex quantification detection with high-sensitivity. Furthermore, our developed platform was also achieved with high-sensitivity and high-specificity through the verification of clinical samples, thus laying out a new avenue for multiplex virus detection in clinical diagnosis. |
| first_indexed | 2024-03-13T10:56:37Z |
| format | Article |
| id | doaj.art-befba65dd3104812a412cd474657bde1 |
| institution | Directory Open Access Journal |
| issn | 2666-1381 |
| language | English |
| last_indexed | 2024-03-13T10:56:37Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Engineered Regeneration |
| spelling | doaj.art-befba65dd3104812a412cd474657bde12023-05-17T04:23:16ZengKeAi Communications Co., Ltd.Engineered Regeneration2666-13812023-09-0143238244One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodesDagan Zhang0Yuze Wang1Junqi Zhao2Xueqin Li3Yuanyang Zhou4Sen Wang5Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China; Department of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China; Corresponding author at: Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, ChinaDepartment of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China; Corresponding author at: Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.Department of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China; Corresponding author at: Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored. PhC barcodes were decorated with PDA by self-polymerization of DA, thus this nanocomposite hybridized PhC barcodes facilitated the adsorption of FITC labelled antibodies and quenched itself photoluminescent, allowing a fast responsive composite platform. In the presence of target analyte, the FITC-labelled detection antibody was released from the surface of PDA decorated microcarrier to specifically bind to the target analyte, thus recovered the photoluminescence. In addition, the PhC microcarrier was enabled to carry out various color barcode for different targets detection though tuning internal periodic structures. Based on these excellent performances of the nanocomposite barcode, this method can not only capture H1N1, H5N1, SARS-CoV-2 simultaneously with rapid, accuracy but also accomplish multiplex quantification detection with high-sensitivity. Furthermore, our developed platform was also achieved with high-sensitivity and high-specificity through the verification of clinical samples, thus laying out a new avenue for multiplex virus detection in clinical diagnosis.http://www.sciencedirect.com/science/article/pii/S2666138123000294BarcodePhotonic crystalsWash-freeImmunoassay |
| spellingShingle | Dagan Zhang Yuze Wang Junqi Zhao Xueqin Li Yuanyang Zhou Sen Wang One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes Engineered Regeneration Barcode Photonic crystals Wash-free Immunoassay |
| title | One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| title_full | One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| title_fullStr | One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| title_full_unstemmed | One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| title_short | One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| title_sort | one step and wash free multiplexed immunoassay platform based on bioinspired photonic barcodes |
| topic | Barcode Photonic crystals Wash-free Immunoassay |
| url | http://www.sciencedirect.com/science/article/pii/S2666138123000294 |
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