Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
Abstract Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endom...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14127 |
| _version_ | 1811197907966099456 |
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| author | He Ding Youyuan Wang Zhiqiang Li Qianqing Li Hongyu Liu Jing Zhao Wenfa Lu Jun Wang |
| author_facet | He Ding Youyuan Wang Zhiqiang Li Qianqing Li Hongyu Liu Jing Zhao Wenfa Lu Jun Wang |
| author_sort | He Ding |
| collection | DOAJ |
| description | Abstract Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endometritis induced by Escherichia coli (E. coli), and then 16S rRNA sequencing and non‐targeted metabolomics were used to investigate the change of uterine microbiota and metabolomes in serum and uterine after BGD treatment. Finally, the therapeutic effect of potential metabolites for treating mouse endometritis screened by combined omics analyses was verified using pathological model. The results showed that BGD treatment could effectively treat endometritis associated with the increasing relative abundance of Firmicutes, Bacteroides, Lactobacillus and Lactococcus, and the decreasing relative abundance of Cupriavidus and Proteobacteria. 133 and 130 metabolites were found to be potential biomarkers in serum and uterine tissue respectively. In serum and tissues, dehydroepiandrosterone (DHEA) and catechol were significantly increased in the BGD treatment versus the inflammation group. Results of combined omics analyses demonstrated that DHEA was positively correlated with changes in microbiota. Results of pathological model demonstrated that DHEA could cure endometritis effectively associated with the decreasing infiltration of inflammatory cells and expression of inflammatory factors in the uterus. In summary, our results demonstrated that BGD could cure endometritis in mice by modulating the structure of the uterine microbiota and its metabolites, in which DHEA may be one of the main components of the therapeutic effect of BGD. |
| first_indexed | 2024-04-12T01:22:05Z |
| format | Article |
| id | doaj.art-befc2239170a41c38cd0b1933c499ec4 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-12T01:22:05Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-befc2239170a41c38cd0b1933c499ec42022-12-22T03:53:45ZengWileyMicrobial Biotechnology1751-79152022-11-0115112786279910.1111/1751-7915.14127Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolitesHe Ding0Youyuan Wang1Zhiqiang Li2Qianqing Li3Hongyu Liu4Jing Zhao5Wenfa Lu6Jun Wang7Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaJoint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education Jilin Agricultural University Changchun ChinaAbstract Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endometritis induced by Escherichia coli (E. coli), and then 16S rRNA sequencing and non‐targeted metabolomics were used to investigate the change of uterine microbiota and metabolomes in serum and uterine after BGD treatment. Finally, the therapeutic effect of potential metabolites for treating mouse endometritis screened by combined omics analyses was verified using pathological model. The results showed that BGD treatment could effectively treat endometritis associated with the increasing relative abundance of Firmicutes, Bacteroides, Lactobacillus and Lactococcus, and the decreasing relative abundance of Cupriavidus and Proteobacteria. 133 and 130 metabolites were found to be potential biomarkers in serum and uterine tissue respectively. In serum and tissues, dehydroepiandrosterone (DHEA) and catechol were significantly increased in the BGD treatment versus the inflammation group. Results of combined omics analyses demonstrated that DHEA was positively correlated with changes in microbiota. Results of pathological model demonstrated that DHEA could cure endometritis effectively associated with the decreasing infiltration of inflammatory cells and expression of inflammatory factors in the uterus. In summary, our results demonstrated that BGD could cure endometritis in mice by modulating the structure of the uterine microbiota and its metabolites, in which DHEA may be one of the main components of the therapeutic effect of BGD.https://doi.org/10.1111/1751-7915.14127 |
| spellingShingle | He Ding Youyuan Wang Zhiqiang Li Qianqing Li Hongyu Liu Jing Zhao Wenfa Lu Jun Wang Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites Microbial Biotechnology |
| title | Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| title_full | Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| title_fullStr | Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| title_full_unstemmed | Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| title_short | Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| title_sort | baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites |
| url | https://doi.org/10.1111/1751-7915.14127 |
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