Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities
Accelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate <i>Eucalyptus</i> oil in a nano-drug carrie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/13/6/604 |
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| author | Nagwa A. Elbhnsawi Bassma H. Elwakil Ahmed H. Hassanin Nader Shehata Salma Sameh Elshewemi Mohamed Hagar Zakia A. Olama |
| author_facet | Nagwa A. Elbhnsawi Bassma H. Elwakil Ahmed H. Hassanin Nader Shehata Salma Sameh Elshewemi Mohamed Hagar Zakia A. Olama |
| author_sort | Nagwa A. Elbhnsawi |
| collection | DOAJ |
| description | Accelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate <i>Eucalyptus</i> oil in a nano-drug carrier to enhance its antimicrobial activity. Furthermore, in vitro, and in vivo wound healing studies of the novel nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate electrospun nanofibers were investigated. <i>Eucalyptus</i> oil showed a potent antimicrobial activity against the tested pathogens and the highest inhibition zone diameter, MIC, and MBC (15.3 mm, 16.0 μg/mL, and 256 μg/mL, respectively) were recorded against <i>Staphylococcus aureus</i>. Data indicated a three-fold increase in the antimicrobial activity of <i>Eucalyptus</i> oil encapsulated chitosan nanoparticle (43 mm inhibition zone diameter against <i>S. aureus</i>). The biosynthesized nanoparticles had a 48.26 nm particle size, 19.0 mV zeta potential, and 0.45 PDI. Electrospinning of nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers was conducted, and the physico-chemical and biological properties revealed that the synthesized nanofibers were homogenous, with a thin diameter (98.0 nm) and a significantly high antimicrobial activity. The in vitro cytotoxic effect in a human normal melanocyte cell line (HFB4) proved an 80% cell viability using 1.5 mg/mL of nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers. In vitro and in vivo wound healing studies revealed that nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers were safe and efficiently enhanced the wound-healing process through enhancing TGF-β, type I and type III collagen production. As a conclusion, the manufactured nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofiber showed effective potentiality for its use as a wound healing dressing. |
| first_indexed | 2024-03-11T02:10:44Z |
| format | Article |
| id | doaj.art-bf03e5ea15874c23a8ae7667fff64b7b |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-11T02:10:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-bf03e5ea15874c23a8ae7667fff64b7b2023-11-18T11:33:55ZengMDPI AGMembranes2077-03752023-06-0113660410.3390/membranes13060604Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing ActivitiesNagwa A. Elbhnsawi0Bassma H. Elwakil1Ahmed H. Hassanin2Nader Shehata3Salma Sameh Elshewemi4Mohamed Hagar5Zakia A. Olama6Department of Botany & Microbiology, Faculty of Science, Alexandria University, Alexandria 21568, EgyptDepartment of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21500, EgyptCentre of Smart Materials, Nanotechnology and Photonics (CSNP), SmartCI Research Centre, Alexandria University, Alexandria 21544, EgyptCentre of Smart Materials, Nanotechnology and Photonics (CSNP), SmartCI Research Centre, Alexandria University, Alexandria 21544, EgyptDepartment of Zoology, Faculty of Science, Alexandria University, Alexandria 21568, EgyptDepartment of Chemistry, Faculty of Science, Alexandria University, Alexandria 21568, EgyptDepartment of Botany & Microbiology, Faculty of Science, Alexandria University, Alexandria 21568, EgyptAccelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate <i>Eucalyptus</i> oil in a nano-drug carrier to enhance its antimicrobial activity. Furthermore, in vitro, and in vivo wound healing studies of the novel nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate electrospun nanofibers were investigated. <i>Eucalyptus</i> oil showed a potent antimicrobial activity against the tested pathogens and the highest inhibition zone diameter, MIC, and MBC (15.3 mm, 16.0 μg/mL, and 256 μg/mL, respectively) were recorded against <i>Staphylococcus aureus</i>. Data indicated a three-fold increase in the antimicrobial activity of <i>Eucalyptus</i> oil encapsulated chitosan nanoparticle (43 mm inhibition zone diameter against <i>S. aureus</i>). The biosynthesized nanoparticles had a 48.26 nm particle size, 19.0 mV zeta potential, and 0.45 PDI. Electrospinning of nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers was conducted, and the physico-chemical and biological properties revealed that the synthesized nanofibers were homogenous, with a thin diameter (98.0 nm) and a significantly high antimicrobial activity. The in vitro cytotoxic effect in a human normal melanocyte cell line (HFB4) proved an 80% cell viability using 1.5 mg/mL of nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers. In vitro and in vivo wound healing studies revealed that nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofibers were safe and efficiently enhanced the wound-healing process through enhancing TGF-β, type I and type III collagen production. As a conclusion, the manufactured nano-chitosan/<i>Eucalyptus</i> oil/cellulose acetate nanofiber showed effective potentiality for its use as a wound healing dressing.https://www.mdpi.com/2077-0375/13/6/604wound dressing nanofibersantibacterial effectinflammationwound healing |
| spellingShingle | Nagwa A. Elbhnsawi Bassma H. Elwakil Ahmed H. Hassanin Nader Shehata Salma Sameh Elshewemi Mohamed Hagar Zakia A. Olama Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities Membranes wound dressing nanofibers antibacterial effect inflammation wound healing |
| title | Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities |
| title_full | Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities |
| title_fullStr | Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities |
| title_full_unstemmed | Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities |
| title_short | Nano-Chitosan/<i>Eucalyptus</i> Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities |
| title_sort | nano chitosan i eucalyptus i oil cellulose acetate nanofibers manufacturing antibacterial and wound healing activities |
| topic | wound dressing nanofibers antibacterial effect inflammation wound healing |
| url | https://www.mdpi.com/2077-0375/13/6/604 |
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