Extreme-value analysis of intracellular cargo transport by motor proteins
Abstract The mechanisms underlying the chemo-mechanical coupling of motor proteins is usually described by a set of force-velocity relations that reflect the different mechanisms responsible for the walking behavior of such proteins on microtubules. However, the convexity of such relations remains c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Communications Physics |
| Online Access: | https://doi.org/10.1038/s42005-024-01538-4 |
| _version_ | 1797274398512644096 |
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| author | Takuma Naoi Yuki Kagawa Kimiko Nagino Shinsuke Niwa Kumiko Hayashi |
| author_facet | Takuma Naoi Yuki Kagawa Kimiko Nagino Shinsuke Niwa Kumiko Hayashi |
| author_sort | Takuma Naoi |
| collection | DOAJ |
| description | Abstract The mechanisms underlying the chemo-mechanical coupling of motor proteins is usually described by a set of force-velocity relations that reflect the different mechanisms responsible for the walking behavior of such proteins on microtubules. However, the convexity of such relations remains controversial depending on the species, and in vivo experiments are inaccessible due to the complexity of intracellular environments. As alternative tool to investigate such mechanism, Extreme-value analysis (EVA) can offer insight on the deviations in the data from the median of the probability distributions. Here, we rely on EVA to investigate the motility functions of nanoscale motor proteins in neurons of the living worm Caenorhabditis elegans (C. elegans), namely the motion of kinesin and dynein along microtubules. While the essential difference between the two motors cannot be inferred from the mean velocities, such becomes evident in the EVA plots. Our findings extend the possibility and applicability of EVA for analysing motility data of nanoscale proteins in vivo. |
| first_indexed | 2024-03-07T14:57:46Z |
| format | Article |
| id | doaj.art-bf0ba925da9e4a819f07b3eecf07ae38 |
| institution | Directory Open Access Journal |
| issn | 2399-3650 |
| language | English |
| last_indexed | 2024-03-07T14:57:46Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Physics |
| spelling | doaj.art-bf0ba925da9e4a819f07b3eecf07ae382024-03-05T19:20:46ZengNature PortfolioCommunications Physics2399-36502024-02-017111010.1038/s42005-024-01538-4Extreme-value analysis of intracellular cargo transport by motor proteinsTakuma Naoi0Yuki Kagawa1Kimiko Nagino2Shinsuke Niwa3Kumiko Hayashi4Department of Applied Physics, Graduate school of Engineering, Tohoku University, Aramaki, Aoba 6-6-05, Aoba-kuDepartment of Applied Physics, Graduate school of Engineering, Tohoku University, Aramaki, Aoba 6-6-05, Aoba-kuDepartment of Applied Physics, Graduate school of Engineering, Tohoku University, Aramaki, Aoba 6-6-05, Aoba-kuFrontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aramaki, Aoba 6-3, Aoba-kuDepartment of Applied Physics, Graduate school of Engineering, Tohoku University, Aramaki, Aoba 6-6-05, Aoba-kuAbstract The mechanisms underlying the chemo-mechanical coupling of motor proteins is usually described by a set of force-velocity relations that reflect the different mechanisms responsible for the walking behavior of such proteins on microtubules. However, the convexity of such relations remains controversial depending on the species, and in vivo experiments are inaccessible due to the complexity of intracellular environments. As alternative tool to investigate such mechanism, Extreme-value analysis (EVA) can offer insight on the deviations in the data from the median of the probability distributions. Here, we rely on EVA to investigate the motility functions of nanoscale motor proteins in neurons of the living worm Caenorhabditis elegans (C. elegans), namely the motion of kinesin and dynein along microtubules. While the essential difference between the two motors cannot be inferred from the mean velocities, such becomes evident in the EVA plots. Our findings extend the possibility and applicability of EVA for analysing motility data of nanoscale proteins in vivo.https://doi.org/10.1038/s42005-024-01538-4 |
| spellingShingle | Takuma Naoi Yuki Kagawa Kimiko Nagino Shinsuke Niwa Kumiko Hayashi Extreme-value analysis of intracellular cargo transport by motor proteins Communications Physics |
| title | Extreme-value analysis of intracellular cargo transport by motor proteins |
| title_full | Extreme-value analysis of intracellular cargo transport by motor proteins |
| title_fullStr | Extreme-value analysis of intracellular cargo transport by motor proteins |
| title_full_unstemmed | Extreme-value analysis of intracellular cargo transport by motor proteins |
| title_short | Extreme-value analysis of intracellular cargo transport by motor proteins |
| title_sort | extreme value analysis of intracellular cargo transport by motor proteins |
| url | https://doi.org/10.1038/s42005-024-01538-4 |
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