Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells.
BACKGROUND: The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangioge...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3247213?pdf=render |
| _version_ | 1798043388879044608 |
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| author | Shijie Tao Merlijn Witte Robert J Bryson-Richardson Peter D Currie Benjamin M Hogan Stefan Schulte-Merker |
| author_facet | Shijie Tao Merlijn Witte Robert J Bryson-Richardson Peter D Currie Benjamin M Hogan Stefan Schulte-Merker |
| author_sort | Shijie Tao |
| collection | DOAJ |
| description | BACKGROUND: The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangiogenesis in mammals. In zebrafish, there are two prox1 paralogues, the previously described prox1 (also known as prox1a) and the newly identified prox1b. PRINCIPAL FINDINGS: To investigate the role of the prox1b gene in zebrafish lymphangiogenesis, we knocked-down prox1b and found that depletion of prox1b mRNA did not cause lymphatic defects. We also generated two different prox1b mutant alleles, and maternal-zygotic homozygous mutant embryos were viable and did not show any lymphatic defects. Furthermore, the expression of prox1b was not restricted to lymphatic vessels during zebrafish development. CONCLUSION: We conclude that Prox1b activity is not essential for embryonic lymphatic development in zebrafish. |
| first_indexed | 2024-04-11T22:49:19Z |
| format | Article |
| id | doaj.art-bf0f42087ae646638c96a68cd55d0581 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:49:19Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bf0f42087ae646638c96a68cd55d05812022-12-22T03:58:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2893410.1371/journal.pone.0028934Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells.Shijie TaoMerlijn WitteRobert J Bryson-RichardsonPeter D CurrieBenjamin M HoganStefan Schulte-MerkerBACKGROUND: The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangiogenesis in mammals. In zebrafish, there are two prox1 paralogues, the previously described prox1 (also known as prox1a) and the newly identified prox1b. PRINCIPAL FINDINGS: To investigate the role of the prox1b gene in zebrafish lymphangiogenesis, we knocked-down prox1b and found that depletion of prox1b mRNA did not cause lymphatic defects. We also generated two different prox1b mutant alleles, and maternal-zygotic homozygous mutant embryos were viable and did not show any lymphatic defects. Furthermore, the expression of prox1b was not restricted to lymphatic vessels during zebrafish development. CONCLUSION: We conclude that Prox1b activity is not essential for embryonic lymphatic development in zebrafish.http://europepmc.org/articles/PMC3247213?pdf=render |
| spellingShingle | Shijie Tao Merlijn Witte Robert J Bryson-Richardson Peter D Currie Benjamin M Hogan Stefan Schulte-Merker Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. PLoS ONE |
| title | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| title_full | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| title_fullStr | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| title_full_unstemmed | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| title_short | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| title_sort | zebrafish prox1b mutants develop a lymphatic vasculature and prox1b does not specifically mark lymphatic endothelial cells |
| url | http://europepmc.org/articles/PMC3247213?pdf=render |
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