Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools
There has been significant interest in soybean oil, fatty acid, and sugar composition to develop new value-added soybean products. Thus, compositional analysis is critical for developing value-added soybeans. In the present study, we showed simple screening tools (near infrared spectroscopy (NIR) an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/8/11/557 |
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| author | Raghavendhar R. Kotha Savithiry Natarajan Dechun Wang Devanand L. Luthria |
| author_facet | Raghavendhar R. Kotha Savithiry Natarajan Dechun Wang Devanand L. Luthria |
| author_sort | Raghavendhar R. Kotha |
| collection | DOAJ |
| description | There has been significant interest in soybean oil, fatty acid, and sugar composition to develop new value-added soybean products. Thus, compositional analysis is critical for developing value-added soybeans. In the present study, we showed simple screening tools (near infrared spectroscopy (NIR) and high-performance thin layer chromatography (HPTLC)) coupled with multivariate analysis for the sample classification of 14 soybeans as a proof-of-concept. We further determined major non-polar and polar metabolites responsible for differences between different soybeans using gas and ion chromatography. These differences in soybean profiles were attributed to lower levels of total oil content in wild soybeans (~9%) versus cultivated soybeans (16%−22%). In addition, higher levels of linolenic acid (~17%) and stachyose (~53%) were determined in wild type, whereas higher levels of oleic acid (~19%) and sucrose (~59%) were detected in cultivated soybeans. Interestingly, one cultivated soybean had a desirable sugar profile with a high amount of sucrose (86%) and a low abundance of stachyose (9%). The correlation studies showed a positive correlation between oil and soluble sugars (R<sup>2</sup> = 0.80) and negative correlations between methyl linolenate and soluble sugars (R<sup>2</sup> = −0.79), oil (R<sup>2</sup> = −0.94), and methyl oleate (R<sup>2</sup> = −0.94) content. Both polar and non-polar metabolites showed significant differences in wild and cultivated soybeans. |
| first_indexed | 2024-04-12T02:01:39Z |
| format | Article |
| id | doaj.art-bf11901a7eea47af95208b6a9ccb09fd |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-12T02:01:39Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bf11901a7eea47af95208b6a9ccb09fd2022-12-22T03:52:40ZengMDPI AGFoods2304-81582019-11-0181155710.3390/foods8110557foods8110557Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography ToolsRaghavendhar R. Kotha0Savithiry Natarajan1Dechun Wang2Devanand L. Luthria3USDA-ARS, Beltsville Human Nutrition Research Center, Beltsville, MD 20705, USAUSDA-ARS, Soybean Genomics and Improvement Laboratory, Beltsville, MD 20705, USADepartment of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USAUSDA-ARS, Beltsville Human Nutrition Research Center, Beltsville, MD 20705, USAThere has been significant interest in soybean oil, fatty acid, and sugar composition to develop new value-added soybean products. Thus, compositional analysis is critical for developing value-added soybeans. In the present study, we showed simple screening tools (near infrared spectroscopy (NIR) and high-performance thin layer chromatography (HPTLC)) coupled with multivariate analysis for the sample classification of 14 soybeans as a proof-of-concept. We further determined major non-polar and polar metabolites responsible for differences between different soybeans using gas and ion chromatography. These differences in soybean profiles were attributed to lower levels of total oil content in wild soybeans (~9%) versus cultivated soybeans (16%−22%). In addition, higher levels of linolenic acid (~17%) and stachyose (~53%) were determined in wild type, whereas higher levels of oleic acid (~19%) and sucrose (~59%) were detected in cultivated soybeans. Interestingly, one cultivated soybean had a desirable sugar profile with a high amount of sucrose (86%) and a low abundance of stachyose (9%). The correlation studies showed a positive correlation between oil and soluble sugars (R<sup>2</sup> = 0.80) and negative correlations between methyl linolenate and soluble sugars (R<sup>2</sup> = −0.79), oil (R<sup>2</sup> = −0.94), and methyl oleate (R<sup>2</sup> = −0.94) content. Both polar and non-polar metabolites showed significant differences in wild and cultivated soybeans.https://www.mdpi.com/2304-8158/8/11/557soybeansmultivariate analysisnon-polar and polar metabolitesion and gas chromatographic separationscorrelation studies |
| spellingShingle | Raghavendhar R. Kotha Savithiry Natarajan Dechun Wang Devanand L. Luthria Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools Foods soybeans multivariate analysis non-polar and polar metabolites ion and gas chromatographic separations correlation studies |
| title | Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools |
| title_full | Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools |
| title_fullStr | Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools |
| title_full_unstemmed | Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools |
| title_short | Compositional Analysis of Non-Polar and Polar Metabolites in 14 Soybeans Using Spectroscopy and Chromatography Tools |
| title_sort | compositional analysis of non polar and polar metabolites in 14 soybeans using spectroscopy and chromatography tools |
| topic | soybeans multivariate analysis non-polar and polar metabolites ion and gas chromatographic separations correlation studies |
| url | https://www.mdpi.com/2304-8158/8/11/557 |
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