Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos.
The recent discovery of a significant amount of RNA in spermatozoa contradicted the previously held belief that paternal contribution was limited to one copy of the genome. Furthermore, detection of RNA in sperm raised the intriguing question of its possible role in embryonic development. The possib...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3440372?pdf=render |
| _version_ | 1819289290599825408 |
|---|---|
| author | Mitsuoki Kawano Hideya Kawaji Valérie Grandjean Jafar Kiani Minoo Rassoulzadegan |
| author_facet | Mitsuoki Kawano Hideya Kawaji Valérie Grandjean Jafar Kiani Minoo Rassoulzadegan |
| author_sort | Mitsuoki Kawano |
| collection | DOAJ |
| description | The recent discovery of a significant amount of RNA in spermatozoa contradicted the previously held belief that paternal contribution was limited to one copy of the genome. Furthermore, detection of RNA in sperm raised the intriguing question of its possible role in embryonic development. The possibility that RNAs may serve as epigenetic determinants was supported by experiments showing inheritance of epigenetic traits in mice mediated by RNA. We used high-throughput, large-scale sequencing technology to analyze sperm RNA. The RNA sequences generated were diverse in terms of length and included mRNAs, rRNAs, piRNAs, and miRNAs. We studied two small noncoding RNAs enriched in mature sperm, designated sperm RNAs (spR) -12 and -13. They are both encoded in a piRNA locus on chromosome 17, but neither their length (20-21 nt), nor their sequences correspond to known piRNAs or miRNAs. They are resistant to periodate-oxidation-mediated reaction, implying that they undergo terminal post-transcriptional modification. Both were detected in sperm and ovulated unfertilized oocytes, present in one-cell embryos and maintained in preimplantation stages, but not at later differentiation stages. These findings offer a new perspective regarding a possibly important role for gamete-specific small RNAs in early embryogenesis. |
| first_indexed | 2024-12-24T03:04:30Z |
| format | Article |
| id | doaj.art-bf155480329245aab3a24b8e73c3ecb1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T03:04:30Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bf155480329245aab3a24b8e73c3ecb12022-12-21T17:18:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4454210.1371/journal.pone.0044542Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos.Mitsuoki KawanoHideya KawajiValérie GrandjeanJafar KianiMinoo RassoulzadeganThe recent discovery of a significant amount of RNA in spermatozoa contradicted the previously held belief that paternal contribution was limited to one copy of the genome. Furthermore, detection of RNA in sperm raised the intriguing question of its possible role in embryonic development. The possibility that RNAs may serve as epigenetic determinants was supported by experiments showing inheritance of epigenetic traits in mice mediated by RNA. We used high-throughput, large-scale sequencing technology to analyze sperm RNA. The RNA sequences generated were diverse in terms of length and included mRNAs, rRNAs, piRNAs, and miRNAs. We studied two small noncoding RNAs enriched in mature sperm, designated sperm RNAs (spR) -12 and -13. They are both encoded in a piRNA locus on chromosome 17, but neither their length (20-21 nt), nor their sequences correspond to known piRNAs or miRNAs. They are resistant to periodate-oxidation-mediated reaction, implying that they undergo terminal post-transcriptional modification. Both were detected in sperm and ovulated unfertilized oocytes, present in one-cell embryos and maintained in preimplantation stages, but not at later differentiation stages. These findings offer a new perspective regarding a possibly important role for gamete-specific small RNAs in early embryogenesis.http://europepmc.org/articles/PMC3440372?pdf=render |
| spellingShingle | Mitsuoki Kawano Hideya Kawaji Valérie Grandjean Jafar Kiani Minoo Rassoulzadegan Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. PLoS ONE |
| title | Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. |
| title_full | Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. |
| title_fullStr | Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. |
| title_full_unstemmed | Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. |
| title_short | Novel small noncoding RNAs in mouse spermatozoa, zygotes and early embryos. |
| title_sort | novel small noncoding rnas in mouse spermatozoa zygotes and early embryos |
| url | http://europepmc.org/articles/PMC3440372?pdf=render |
| work_keys_str_mv | AT mitsuokikawano novelsmallnoncodingrnasinmousespermatozoazygotesandearlyembryos AT hideyakawaji novelsmallnoncodingrnasinmousespermatozoazygotesandearlyembryos AT valeriegrandjean novelsmallnoncodingrnasinmousespermatozoazygotesandearlyembryos AT jafarkiani novelsmallnoncodingrnasinmousespermatozoazygotesandearlyembryos AT minoorassoulzadegan novelsmallnoncodingrnasinmousespermatozoazygotesandearlyembryos |