Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2)
Background Percutaneous image-guided tissue ablation (IGA) plays a growing role in the clinical management of solid malignancies. Electroporation is used for IGA in several modalities: irreversible electroporation (IRE), and reversible electroporation with chemotoxic drugs, called electrochemotherap...
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PeerJ Inc.
2020-01-01
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| Online Access: | https://peerj.com/articles/7985.pdf |
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| author | Enric Guenther Nina Klein Paul Mikus Florin Botea Mihail Pautov Franco Lugnani Matteo Macchioro Irinel Popescu Michael K. Stehling Boris Rubinsky |
| author_facet | Enric Guenther Nina Klein Paul Mikus Florin Botea Mihail Pautov Franco Lugnani Matteo Macchioro Irinel Popescu Michael K. Stehling Boris Rubinsky |
| author_sort | Enric Guenther |
| collection | DOAJ |
| description | Background Percutaneous image-guided tissue ablation (IGA) plays a growing role in the clinical management of solid malignancies. Electroporation is used for IGA in several modalities: irreversible electroporation (IRE), and reversible electroporation with chemotoxic drugs, called electrochemotherapy (ECT). It was shown that the combination of electrolysis and electroporation—E2—affords tissue ablation with greater efficiency, that is, lower voltages, lower energy and shorter procedure times than IRE and without the need for chemotoxic additives as in ECT. Methods A new E2 waveform was designed that delivers optimal doses of electroporation and electrolysis in a single waveform. A series of experiments were performed in the liver of pigs to evaluate E2 in the context of clinical applications. The goal was to find initial parameter boundaries in terms of electrical field, pulse duration and charge as well as tissue behavior to enable real time tissue ablation of clinically relevant volumes. Results Histological results show that a single several hundred millisecond long E2 waveform can ablate large volume of tissue at relatively low voltages while preserving the integrity of large blood vessels and lumen structures in the ablation zone without the use of chemotoxic drugs or paralyzing drugs during anesthesia. This could translate clinically into much shorter treatment times and ease of use compared to other techniques that are currently applied. |
| first_indexed | 2024-03-09T06:47:33Z |
| format | Article |
| id | doaj.art-bf1780fa5aed4fa0aa12fb79f0f4667e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:47:33Z |
| publishDate | 2020-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-bf1780fa5aed4fa0aa12fb79f0f4667e2023-12-03T10:34:29ZengPeerJ Inc.PeerJ2167-83592020-01-018e798510.7717/peerj.7985Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2)Enric Guenther0Nina Klein1Paul Mikus2Florin Botea3Mihail Pautov4Franco Lugnani5Matteo Macchioro6Irinel Popescu7Michael K. Stehling8Boris Rubinsky9Biophysics, Inter Science GmbH, Gisikon, Lucerne, SwitzerlandBiophysics, Inter Science GmbH, Gisikon, Lucerne, SwitzerlandBiophysics, Inter Science GmbH, Gisikon, Lucerne, SwitzerlandCenter of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest, RomaniaCenter of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest, RomaniaHippocrates D.O.O. Divaĉa, Malaga, SpainHippocrates D.O.O. Divaĉa, Malaga, SpainCenter of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest, RomaniaBiophysics, Inter Science GmbH, Gisikon, Lucerne, SwitzerlandBiophysics, Inter Science GmbH, Gisikon, Lucerne, SwitzerlandBackground Percutaneous image-guided tissue ablation (IGA) plays a growing role in the clinical management of solid malignancies. Electroporation is used for IGA in several modalities: irreversible electroporation (IRE), and reversible electroporation with chemotoxic drugs, called electrochemotherapy (ECT). It was shown that the combination of electrolysis and electroporation—E2—affords tissue ablation with greater efficiency, that is, lower voltages, lower energy and shorter procedure times than IRE and without the need for chemotoxic additives as in ECT. Methods A new E2 waveform was designed that delivers optimal doses of electroporation and electrolysis in a single waveform. A series of experiments were performed in the liver of pigs to evaluate E2 in the context of clinical applications. The goal was to find initial parameter boundaries in terms of electrical field, pulse duration and charge as well as tissue behavior to enable real time tissue ablation of clinically relevant volumes. Results Histological results show that a single several hundred millisecond long E2 waveform can ablate large volume of tissue at relatively low voltages while preserving the integrity of large blood vessels and lumen structures in the ablation zone without the use of chemotoxic drugs or paralyzing drugs during anesthesia. This could translate clinically into much shorter treatment times and ease of use compared to other techniques that are currently applied.https://peerj.com/articles/7985.pdfE2ElectroporationElectrolysisIrreversible electroporationIREElectro chemo theraphy |
| spellingShingle | Enric Guenther Nina Klein Paul Mikus Florin Botea Mihail Pautov Franco Lugnani Matteo Macchioro Irinel Popescu Michael K. Stehling Boris Rubinsky Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) PeerJ E2 Electroporation Electrolysis Irreversible electroporation IRE Electro chemo theraphy |
| title | Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) |
| title_full | Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) |
| title_fullStr | Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) |
| title_full_unstemmed | Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) |
| title_short | Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2) |
| title_sort | toward a clinical real time tissue ablation technology combining electroporation and electrolysis e2 |
| topic | E2 Electroporation Electrolysis Irreversible electroporation IRE Electro chemo theraphy |
| url | https://peerj.com/articles/7985.pdf |
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