The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species
Cortisol carries out its physiological mechanism of action through the recognition by the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR) 1 (GR1) and GR2. Previous studies reported that the main difference between gr1 and gr2 nucleotide sequences resides in a 27-nucleotide inser...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1021046/full |
| _version_ | 1811239115415355392 |
|---|---|
| author | Eva Vallejos-Vidal Ali Reza Khansari Mariana Teles Mariana Teles Sebastián Reyes-Cerpa Sebastián Reyes-Cerpa Juan Miguel Mancera Lluis Tort Felipe E. Reyes-López |
| author_facet | Eva Vallejos-Vidal Ali Reza Khansari Mariana Teles Mariana Teles Sebastián Reyes-Cerpa Sebastián Reyes-Cerpa Juan Miguel Mancera Lluis Tort Felipe E. Reyes-López |
| author_sort | Eva Vallejos-Vidal |
| collection | DOAJ |
| description | Cortisol carries out its physiological mechanism of action through the recognition by the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR) 1 (GR1) and GR2. Previous studies reported that the main difference between gr1 and gr2 nucleotide sequences resides in a 27-nucleotide insertion/deletion in the DNA-binding region, respectively. However, in gilthead sea bream (Sparus aurata) the annotation for gr1 and gr2 seems contradictory. The gr2 sequence possesses the characteristic 27-nucleotide insertion that, in fact, is associated with the gr1 nucleotide sequence. Thus, this study aimed to elucidate the nucleotide sequences for the gr1 and gr2 in gilthead sea bream. The Clustal Omega alignment for different fish species corroborated the presence of such 27-nucleotide insertion/deletion in the DNA-binding region for gr1 and gr2, respectively. Then, we design specific primers set for the amplification of the gilthead sea bream gr1 by polymerase chain reaction (PCR). Importantly, the gr1 nucleotide partial sequence has a high similarity with other gr1 sequences already published for other fish species, being present in all of them the 27-nucleotide insertion in the DNA-binding region. We also detected that in European sea bass the gr1 and gr2 sequences had not been named according to the 27-nucleotide insertion/deletion criteria in the DNA-binding region. Thus, our study makes an urgent call to the scientific community to discuss the establishment of an updated agreement that allows homogenizing the criteria for the nomenclature defining the gr1 and gr2 nucleotide sequences for a better understanding of the stress response in teleost fish species. |
| first_indexed | 2024-04-12T12:54:11Z |
| format | Article |
| id | doaj.art-bf17e9cd2a9749b8be5de9b251eea87b |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-12T12:54:11Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-bf17e9cd2a9749b8be5de9b251eea87b2022-12-22T03:32:22ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-10-01910.3389/fmars.2022.10210461021046The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish speciesEva Vallejos-Vidal0Ali Reza Khansari1Mariana Teles2Mariana Teles3Sebastián Reyes-Cerpa4Sebastián Reyes-Cerpa5Juan Miguel Mancera6Lluis Tort7Felipe E. Reyes-López8Núcleo de Investigación Aplicada en Ciencias Veterinarias y Agronómicas, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago, ChileDepartment of Cell Biology, Physiology, and Immunology, Universitat Autònoma de Barcelona, Bellaterra, SpainDepartment of Cell Biology, Physiology, and Immunology, Universitat Autònoma de Barcelona, Bellaterra, SpainInstitute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, Barcelona, SpainCentro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago, ChileEscuela de Biotecnología, Facultad de Ciencias, Universidad Mayor, Santiago, ChileDepartment of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, Cádiz, SpainDepartment of Cell Biology, Physiology, and Immunology, Universitat Autònoma de Barcelona, Bellaterra, SpainCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileCortisol carries out its physiological mechanism of action through the recognition by the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR) 1 (GR1) and GR2. Previous studies reported that the main difference between gr1 and gr2 nucleotide sequences resides in a 27-nucleotide insertion/deletion in the DNA-binding region, respectively. However, in gilthead sea bream (Sparus aurata) the annotation for gr1 and gr2 seems contradictory. The gr2 sequence possesses the characteristic 27-nucleotide insertion that, in fact, is associated with the gr1 nucleotide sequence. Thus, this study aimed to elucidate the nucleotide sequences for the gr1 and gr2 in gilthead sea bream. The Clustal Omega alignment for different fish species corroborated the presence of such 27-nucleotide insertion/deletion in the DNA-binding region for gr1 and gr2, respectively. Then, we design specific primers set for the amplification of the gilthead sea bream gr1 by polymerase chain reaction (PCR). Importantly, the gr1 nucleotide partial sequence has a high similarity with other gr1 sequences already published for other fish species, being present in all of them the 27-nucleotide insertion in the DNA-binding region. We also detected that in European sea bass the gr1 and gr2 sequences had not been named according to the 27-nucleotide insertion/deletion criteria in the DNA-binding region. Thus, our study makes an urgent call to the scientific community to discuss the establishment of an updated agreement that allows homogenizing the criteria for the nomenclature defining the gr1 and gr2 nucleotide sequences for a better understanding of the stress response in teleost fish species.https://www.frontiersin.org/articles/10.3389/fmars.2022.1021046/fullstress responsecortisolglucocorticoid receptors (GRs)glucocorticoid receptor 1 (GR1)glucocorticoid receptor 2 (GR2)perciformes fish species |
| spellingShingle | Eva Vallejos-Vidal Ali Reza Khansari Mariana Teles Mariana Teles Sebastián Reyes-Cerpa Sebastián Reyes-Cerpa Juan Miguel Mancera Lluis Tort Felipe E. Reyes-López The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species Frontiers in Marine Science stress response cortisol glucocorticoid receptors (GRs) glucocorticoid receptor 1 (GR1) glucocorticoid receptor 2 (GR2) perciformes fish species |
| title | The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| title_full | The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| title_fullStr | The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| title_full_unstemmed | The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| title_short | The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| title_sort | insertion deletion in the dna binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 gr1 and gr2 nucleotide sequences in gilthead sea bream sparus aurata standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species |
| topic | stress response cortisol glucocorticoid receptors (GRs) glucocorticoid receptor 1 (GR1) glucocorticoid receptor 2 (GR2) perciformes fish species |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1021046/full |
| work_keys_str_mv | AT evavallejosvidal theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT alirezakhansari theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT marianateles theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT marianateles theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT sebastianreyescerpa theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT sebastianreyescerpa theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT juanmiguelmancera theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT lluistort theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT felipeereyeslopez theinsertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseintele AT evavallejosvidal insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT alirezakhansari insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT marianateles insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT marianateles insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT sebastianreyescerpa insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT sebastianreyescerpa insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT juanmiguelmancera insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT lluistort insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost AT felipeereyeslopez insertiondeletioninthednabindingregionallowsthediscriminationandsubsequentidentificationoftheglucocorticoidreceptor1gr1andgr2nucleotidesequencesingiltheadseabreamsparusauratastandardizingthegrnomenclatureforabetterunderstandingofthestressresponseinteleost |