High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes
Acute bouts of high-intensity intermittent exercise (HIIE) or sports are associated with changes in lymphocytes and platelet functions and we hypothesized that the purinergic system is involved with these alterations. We investigated the activity of ectonucleotidases in platelets and lymphocytes as...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-10-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2018.1529299 |
| _version_ | 1797684292255481856 |
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| author | Vanessa Valéria Miron Jucimara Baldissarelli Gabriel Pranke Aline Mânica Beatriz da Silva Rosa Bonadiman Leandro Henrique Manfredi Vera Maria Morsch Maria Rosa Chitolina Schetinger Andréia Machado Cardoso |
| author_facet | Vanessa Valéria Miron Jucimara Baldissarelli Gabriel Pranke Aline Mânica Beatriz da Silva Rosa Bonadiman Leandro Henrique Manfredi Vera Maria Morsch Maria Rosa Chitolina Schetinger Andréia Machado Cardoso |
| author_sort | Vanessa Valéria Miron |
| collection | DOAJ |
| description | Acute bouts of high-intensity intermittent exercise (HIIE) or sports are associated with changes in lymphocytes and platelet functions and we hypothesized that the purinergic system is involved with these alterations. We investigated the activity of ectonucleotidases in platelets and lymphocytes as well as the platelet aggregation of futsal players in response to an acute protocol of HIIE. Thus, 19 male semi-professional futsal players were submitted to 40 min of HIIE on a treadmill. Blood samples were collected three-time points: before exercise, immediately after, and 30 min after the end of the session. Platelet-rich plasma (PRP) and lymphocytes were isolated. ATP, ADP, AMP, and adenosine hydrolysis, NTPDase1 (CD39) expression as well as platelet aggregation were measured. Our results showed HIIE induced a decrease in ATP and ADP hydrolysis in platelets, an increase in adenosine hydrolysis and an increase in platelet aggregation immediately after exercise. After 30 min of recovery, enzymatic activity and platelet aggregation returned to baseline levels. In lymphocytes, adenosine hydrolysis was augmented immediately after exercise and remained increased even after 30 min of recovery. In conclusion, acute HIIE triggers a transient proaggregant status that is reverted after a 30 min of recovery. The effects of HIIE in lymphocytes remained after 30 min of recovery, indicating a pro-inflammatory response. This work elucidated some of the mechanisms by which purinergic system regulates lymphocytes and platelets activities related to HIIE, suggesting that the type of exercise may influence an increase in platelet aggregation even in trained individuals. |
| first_indexed | 2024-03-12T00:27:26Z |
| format | Article |
| id | doaj.art-bf1cef27515549f89cbe42d2dcc050b5 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:26Z |
| publishDate | 2019-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-bf1cef27515549f89cbe42d2dcc050b52023-09-15T10:32:00ZengTaylor & Francis GroupPlatelets0953-71041369-16352019-10-0130787888510.1080/09537104.2018.15292991529299High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletesVanessa Valéria Miron0Jucimara Baldissarelli1Gabriel Pranke2Aline Mânica3Beatriz da Silva Rosa Bonadiman4Leandro Henrique Manfredi5Vera Maria Morsch6Maria Rosa Chitolina Schetinger7Andréia Machado Cardoso8Federal University of Santa MariaFederal University of Santa MariaFederal University of Santa MariaFederal University of Santa MariaFederal University of Santa MariaFederal University of Fronteira SulFederal University of Santa MariaFederal University of Santa MariaFederal University of Fronteira SulAcute bouts of high-intensity intermittent exercise (HIIE) or sports are associated with changes in lymphocytes and platelet functions and we hypothesized that the purinergic system is involved with these alterations. We investigated the activity of ectonucleotidases in platelets and lymphocytes as well as the platelet aggregation of futsal players in response to an acute protocol of HIIE. Thus, 19 male semi-professional futsal players were submitted to 40 min of HIIE on a treadmill. Blood samples were collected three-time points: before exercise, immediately after, and 30 min after the end of the session. Platelet-rich plasma (PRP) and lymphocytes were isolated. ATP, ADP, AMP, and adenosine hydrolysis, NTPDase1 (CD39) expression as well as platelet aggregation were measured. Our results showed HIIE induced a decrease in ATP and ADP hydrolysis in platelets, an increase in adenosine hydrolysis and an increase in platelet aggregation immediately after exercise. After 30 min of recovery, enzymatic activity and platelet aggregation returned to baseline levels. In lymphocytes, adenosine hydrolysis was augmented immediately after exercise and remained increased even after 30 min of recovery. In conclusion, acute HIIE triggers a transient proaggregant status that is reverted after a 30 min of recovery. The effects of HIIE in lymphocytes remained after 30 min of recovery, indicating a pro-inflammatory response. This work elucidated some of the mechanisms by which purinergic system regulates lymphocytes and platelets activities related to HIIE, suggesting that the type of exercise may influence an increase in platelet aggregation even in trained individuals.http://dx.doi.org/10.1080/09537104.2018.1529299athletesectonucleotidasesintense physical exercisepurinergic system |
| spellingShingle | Vanessa Valéria Miron Jucimara Baldissarelli Gabriel Pranke Aline Mânica Beatriz da Silva Rosa Bonadiman Leandro Henrique Manfredi Vera Maria Morsch Maria Rosa Chitolina Schetinger Andréia Machado Cardoso High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes Platelets athletes ectonucleotidases intense physical exercise purinergic system |
| title | High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| title_full | High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| title_fullStr | High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| title_full_unstemmed | High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| title_short | High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| title_sort | high intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes |
| topic | athletes ectonucleotidases intense physical exercise purinergic system |
| url | http://dx.doi.org/10.1080/09537104.2018.1529299 |
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