Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer
Background: Src is a non-receptor protein tyrosine kinase that has a crucial role in signaling pathways involved in cell division, motility, adhesion, and survival in cells. To date, there has been no report on the association between polymorphism of CA dinucleotide repeat in the c-src gene and risk...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2018-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/8171 |
| _version_ | 1797720936183496704 |
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| author | Mohammad Amin Ezhehi Manoochehr Tavassoli Simin Hematti Forouzan Safari |
| author_facet | Mohammad Amin Ezhehi Manoochehr Tavassoli Simin Hematti Forouzan Safari |
| author_sort | Mohammad Amin Ezhehi |
| collection | DOAJ |
| description | Background: Src is a non-receptor protein tyrosine kinase that has a crucial role in signaling pathways involved in cell division, motility, adhesion, and survival in cells. To date, there has been no report on the association between polymorphism of CA dinucleotide repeat in the c-src gene and risk of prostate cancer. In this study, the association between CA polymorphism in intron 2 of the c-src gene and the risk of prostate cancer was investigated.
Methods: A group of 142 patients with prostate cancer and 135 healthy men were selected. Genomic DNA was extracted from white blood cells and desired sequence was amplified via the polymerase chain reaction (PCR). The number of CA repetitions was obtained using polyacrylamide gel electrophoresis and sequencing.
Findings: 15 different alleles were identified in 277 subjects; their distribution varied between 13 and 28 repeats. 22 repeat allele was associated with an increased risk of prostate cancer [Odds ratio (OR) = 2.31, P = 0.0005] and men with genotypes 22-CA/20-CA and 22-CA/19-CA were at higher risk of developing prostate cancer (OR = 5.5, P = 0.0001).
Conclusion: Our findings demonstrate that men with 22 CA repeat are at a significantly higher risk of prostate cancer. In conclusion, our result suggests a potential role for this microsatellite as a predictive marker of prostate cancer risk in Iranian men. |
| first_indexed | 2024-03-12T09:27:38Z |
| format | Article |
| id | doaj.art-bf1e8fbe7fc74bc4a56059a0d19a57d2 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:27:38Z |
| publishDate | 2018-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-bf1e8fbe7fc74bc4a56059a0d19a57d22023-09-02T14:08:47ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2018-01-01354541587159210.22122/jims.v35i454.81712713Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate CancerMohammad Amin Ezhehi0Manoochehr Tavassoli1Simin Hematti2Forouzan Safari3MSc Student, Department of Biology, School of Sciences, University of Isfahan, Isfahan, IranProfessor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, IranAssistant Professor, Department of Radiotherapy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Biology, School of Sciences, University of Isfahan, Isfahan, IranBackground: Src is a non-receptor protein tyrosine kinase that has a crucial role in signaling pathways involved in cell division, motility, adhesion, and survival in cells. To date, there has been no report on the association between polymorphism of CA dinucleotide repeat in the c-src gene and risk of prostate cancer. In this study, the association between CA polymorphism in intron 2 of the c-src gene and the risk of prostate cancer was investigated. Methods: A group of 142 patients with prostate cancer and 135 healthy men were selected. Genomic DNA was extracted from white blood cells and desired sequence was amplified via the polymerase chain reaction (PCR). The number of CA repetitions was obtained using polyacrylamide gel electrophoresis and sequencing. Findings: 15 different alleles were identified in 277 subjects; their distribution varied between 13 and 28 repeats. 22 repeat allele was associated with an increased risk of prostate cancer [Odds ratio (OR) = 2.31, P = 0.0005] and men with genotypes 22-CA/20-CA and 22-CA/19-CA were at higher risk of developing prostate cancer (OR = 5.5, P = 0.0001). Conclusion: Our findings demonstrate that men with 22 CA repeat are at a significantly higher risk of prostate cancer. In conclusion, our result suggests a potential role for this microsatellite as a predictive marker of prostate cancer risk in Iranian men.http://jims.mui.ac.ir/index.php/jims/article/view/8171Prostate cancerc-src genes, PolymorphismMicrosatellites |
| spellingShingle | Mohammad Amin Ezhehi Manoochehr Tavassoli Simin Hematti Forouzan Safari Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer مجله دانشکده پزشکی اصفهان Prostate cancer c-src genes, Polymorphism Microsatellites |
| title | Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer |
| title_full | Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer |
| title_fullStr | Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer |
| title_full_unstemmed | Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer |
| title_short | Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer |
| title_sort | association between the length of a ca dinucleotide repeat in the c src gene and risk of prostate cancer |
| topic | Prostate cancer c-src genes, Polymorphism Microsatellites |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/8171 |
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