Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems.
Galla rhois is a commonly used traditional medicine for the treatment of pathogenic bacteria in Korea as well as in other parts of Asia. Methyl gallate (MG), a major component of Galla Rhois, exhibits strong antibacterial activity, but its mechanism of action against Salmonella spp. is unclear. In t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4105534?pdf=render |
| _version_ | 1811219989405892608 |
|---|---|
| author | Jang-Gi Choi Su-Hyun Mun Harendra S Chahar Preeti Bharaj Ok-Hwa Kang Se-Gun Kim Dong-Won Shin Dong-Yeul Kwon |
| author_facet | Jang-Gi Choi Su-Hyun Mun Harendra S Chahar Preeti Bharaj Ok-Hwa Kang Se-Gun Kim Dong-Won Shin Dong-Yeul Kwon |
| author_sort | Jang-Gi Choi |
| collection | DOAJ |
| description | Galla rhois is a commonly used traditional medicine for the treatment of pathogenic bacteria in Korea as well as in other parts of Asia. Methyl gallate (MG), a major component of Galla Rhois, exhibits strong antibacterial activity, but its mechanism of action against Salmonella spp. is unclear. In the present study, we investigated the antibacterial actions of MG against Salmonella. The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of MG against Salmonella strains ranged from 3.9 to 125 µg/ml. In vitro bacterial viability test indicated that MG significantly decreased the viability of Salmonella over 40% when combined with ATPase inhibitors. The time-kill curves showed that a combined MG and ATPase inhibitors (DCCD and NaN3) treatment reduced the bacterial counts dramatically after 24 h. Oral administration of MG showed a strong anti-bacterial activity against WS-5 infected BALB/c mice. In contrast to the untreated Salmonella infected control animals, MG treated groups showed no clinical symptoms of the disease, such as lethargy and liver damage. It was observed that MG treatment significantly increased the survival of animals from Salmonella infection, while in untreated groups all animal succumbed to disease by the sixth day post infection. Thus, the present study demonstrates the therapeutic ability of MG against Salmonella infections. |
| first_indexed | 2024-04-12T07:34:55Z |
| format | Article |
| id | doaj.art-bf1f9ff3353d4fba97a1f728a91a891b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:34:55Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bf1f9ff3353d4fba97a1f728a91a891b2022-12-22T03:41:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10269710.1371/journal.pone.0102697Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems.Jang-Gi ChoiSu-Hyun MunHarendra S ChaharPreeti BharajOk-Hwa KangSe-Gun KimDong-Won ShinDong-Yeul KwonGalla rhois is a commonly used traditional medicine for the treatment of pathogenic bacteria in Korea as well as in other parts of Asia. Methyl gallate (MG), a major component of Galla Rhois, exhibits strong antibacterial activity, but its mechanism of action against Salmonella spp. is unclear. In the present study, we investigated the antibacterial actions of MG against Salmonella. The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of MG against Salmonella strains ranged from 3.9 to 125 µg/ml. In vitro bacterial viability test indicated that MG significantly decreased the viability of Salmonella over 40% when combined with ATPase inhibitors. The time-kill curves showed that a combined MG and ATPase inhibitors (DCCD and NaN3) treatment reduced the bacterial counts dramatically after 24 h. Oral administration of MG showed a strong anti-bacterial activity against WS-5 infected BALB/c mice. In contrast to the untreated Salmonella infected control animals, MG treated groups showed no clinical symptoms of the disease, such as lethargy and liver damage. It was observed that MG treatment significantly increased the survival of animals from Salmonella infection, while in untreated groups all animal succumbed to disease by the sixth day post infection. Thus, the present study demonstrates the therapeutic ability of MG against Salmonella infections.http://europepmc.org/articles/PMC4105534?pdf=render |
| spellingShingle | Jang-Gi Choi Su-Hyun Mun Harendra S Chahar Preeti Bharaj Ok-Hwa Kang Se-Gun Kim Dong-Won Shin Dong-Yeul Kwon Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. PLoS ONE |
| title | Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. |
| title_full | Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. |
| title_fullStr | Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. |
| title_full_unstemmed | Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. |
| title_short | Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. |
| title_sort | methyl gallate from galla rhois successfully controls clinical isolates of salmonella infection in both in vitro and in vivo systems |
| url | http://europepmc.org/articles/PMC4105534?pdf=render |
| work_keys_str_mv | AT janggichoi methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT suhyunmun methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT harendraschahar methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT preetibharaj methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT okhwakang methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT segunkim methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT dongwonshin methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems AT dongyeulkwon methylgallatefromgallarhoissuccessfullycontrolsclinicalisolatesofsalmonellainfectioninbothinvitroandinvivosystems |