Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake
Abstract In summer, the survival zones of cold‐water species are predicted to narrow by both increasing water temperatures from the surface and by expanding hypoxic zones from the lake bottom. To examine how the abundance of cold‐water fishes changes along environmental gradients, we assessed the ve...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.11091 |
| _version_ | 1797244938433331200 |
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| author | Kayoko Fukumori Natsuko I. Kondo Ayato Kohzu Kenji Tsuchiya Hiroshi Ito Taku Kadoya |
| author_facet | Kayoko Fukumori Natsuko I. Kondo Ayato Kohzu Kenji Tsuchiya Hiroshi Ito Taku Kadoya |
| author_sort | Kayoko Fukumori |
| collection | DOAJ |
| description | Abstract In summer, the survival zones of cold‐water species are predicted to narrow by both increasing water temperatures from the surface and by expanding hypoxic zones from the lake bottom. To examine how the abundance of cold‐water fishes changes along environmental gradients, we assessed the vertical environmental DNA (eDNA) distributions of three salmonid species which may have different water temperature tolerances during both stratification and turnover periods using quantitative PCR (qPCR). In addition, we examined on the vertical distribution of diverse fish fauna using an eDNA metabarcoding assay. The results suggested that the kokanee salmon (Oncorhynchus nerka) eDNA were abundant in deep, cold waters. On the other hand, rainbow trout (O. mykiss) eDNA were distributed uniformly throughout the water column, suggesting that they may have high water‐temperature tolerance compared with kokanee salmon. The eDNA concentrations of masu salmon (O. masou) were below the detection limit (i.e., <10 copies μL−1) at all stations and depths and hence could not be quantified during stratification. Together with the finding that the eDNA distributions of other prey fish species were also constrained vertically in species‐specific ways, our results suggest that climate change will result in substantial changes in the vertical distributions of lake fish species and thus affect their populations and interactions. |
| first_indexed | 2024-04-24T19:18:58Z |
| format | Article |
| id | doaj.art-bf2398dcac1046bc8852359165d5dfac |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-24T19:18:58Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-bf2398dcac1046bc8852359165d5dfac2024-03-26T04:26:57ZengWileyEcology and Evolution2045-77582024-03-01143n/an/a10.1002/ece3.11091Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lakeKayoko Fukumori0Natsuko I. Kondo1Ayato Kohzu2Kenji Tsuchiya3Hiroshi Ito4Taku Kadoya5Biodiversity Division National Institute for Environmental Studies (NIES) Ibaraki JapanBiodiversity Division National Institute for Environmental Studies (NIES) Ibaraki JapanRegional Environment Conservation Division National Institute for Environmental Studies (NIES) Ibaraki JapanRegional Environment Conservation Division National Institute for Environmental Studies (NIES) Ibaraki JapanBiodiversity Division National Institute for Environmental Studies (NIES) Ibaraki JapanBiodiversity Division National Institute for Environmental Studies (NIES) Ibaraki JapanAbstract In summer, the survival zones of cold‐water species are predicted to narrow by both increasing water temperatures from the surface and by expanding hypoxic zones from the lake bottom. To examine how the abundance of cold‐water fishes changes along environmental gradients, we assessed the vertical environmental DNA (eDNA) distributions of three salmonid species which may have different water temperature tolerances during both stratification and turnover periods using quantitative PCR (qPCR). In addition, we examined on the vertical distribution of diverse fish fauna using an eDNA metabarcoding assay. The results suggested that the kokanee salmon (Oncorhynchus nerka) eDNA were abundant in deep, cold waters. On the other hand, rainbow trout (O. mykiss) eDNA were distributed uniformly throughout the water column, suggesting that they may have high water‐temperature tolerance compared with kokanee salmon. The eDNA concentrations of masu salmon (O. masou) were below the detection limit (i.e., <10 copies μL−1) at all stations and depths and hence could not be quantified during stratification. Together with the finding that the eDNA distributions of other prey fish species were also constrained vertically in species‐specific ways, our results suggest that climate change will result in substantial changes in the vertical distributions of lake fish species and thus affect their populations and interactions.https://doi.org/10.1002/ece3.11091eDNAmetabarcodingsalmonspecies detectionstratification |
| spellingShingle | Kayoko Fukumori Natsuko I. Kondo Ayato Kohzu Kenji Tsuchiya Hiroshi Ito Taku Kadoya Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake Ecology and Evolution eDNA metabarcoding salmon species detection stratification |
| title | Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake |
| title_full | Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake |
| title_fullStr | Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake |
| title_full_unstemmed | Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake |
| title_short | Vertical eDNA distribution of cold‐water fishes in response to environmental variables in stratified lake |
| title_sort | vertical edna distribution of cold water fishes in response to environmental variables in stratified lake |
| topic | eDNA metabarcoding salmon species detection stratification |
| url | https://doi.org/10.1002/ece3.11091 |
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