Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep
Background: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well d...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2022-07-01
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| Series: | Revista Colombiana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/rccp/article/view/347740 |
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| author | María Guadalupe Martínez-Serrano Efrén Díaz-Aparicio Gabriela Palomares-Reséndiz Jorge Luis Tórtora-Pérez Hugo Ramírez-Álvarez Nieves Ortega-Hernández Jesús Salinas-Lorente José Francisco Morales-Alvarez Juan Julio César Cervantes-Morali |
| author_facet | María Guadalupe Martínez-Serrano Efrén Díaz-Aparicio Gabriela Palomares-Reséndiz Jorge Luis Tórtora-Pérez Hugo Ramírez-Álvarez Nieves Ortega-Hernández Jesús Salinas-Lorente José Francisco Morales-Alvarez Juan Julio César Cervantes-Morali |
| author_sort | María Guadalupe Martínez-Serrano |
| collection | DOAJ |
| description | Background: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well documented. However, there are no reports about isolation and identification of C. abortus in the colostrum or milk of infected sheep, so it is important to determine whether or not C. abortus may be present in these secretions, which are the only food of lambs. Objective: To detect C. abortus in colostrum, milk, and vaginal secretions of sheep with a history of reproductive disorders. Methods: Colostrum, milk, and vaginal exudates were collected from 66 sheep. The samples were inoculated in mouse fibroblast cell cultures and the presence of C. abortus determined by direct immunofluorescence. Results: 19 out of 66 colostrum samples (28.7%), 14 out of 66 milk samples (21.2%) and 17 out of 66 vaginal swabs (25.7%) were positive for C. abortus. The 50 samples positive for isolation and detected by immunofluorescence, together with 42 negative samples were subjected to qPCR to amplify a fragment of the ompA gene from C. abortus. Thirty-eight of the 92 samples processed by this technique were positive for C. abortus. Conclusion: The results demonstrated the presence of C. abortus in a high proportion in colostrum, milk and vaginal secretions of infected sheep. To the best of our knowledge, this is the first field study confirming the presence of C. abortus in colostrum, which shows that excretion of Chlamydia by lactogenesis could occur in the first hours after birth. |
| first_indexed | 2024-03-08T21:40:26Z |
| format | Article |
| id | doaj.art-bf25b715544e4e60a59474125f64a006 |
| institution | Directory Open Access Journal |
| issn | 2256-2958 |
| language | English |
| last_indexed | 2024-03-08T21:40:26Z |
| publishDate | 2022-07-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Colombiana de Ciencias Pecuarias |
| spelling | doaj.art-bf25b715544e4e60a59474125f64a0062023-12-20T13:51:20ZengUniversidad de AntioquiaRevista Colombiana de Ciencias Pecuarias2256-29582022-07-0135316517310.17533/udea.rccp.v35n2a0444506Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheepMaría Guadalupe Martínez-Serrano0Efrén Díaz-Aparicio1Gabriela Palomares-Reséndiz2Jorge Luis Tórtora-Pérez3Hugo Ramírez-Álvarez4Nieves Ortega-Hernández5Jesús Salinas-Lorente6José Francisco Morales-Alvarez7Juan Julio César Cervantes-Morali8Universidad Nacional Autónoma de MéxicoInstituto Nacional de Investigaciones Forestales Agrícolas y PecuariasInstituto Nacional de Investigaciones Forestales Agrícolas y PecuariasUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad de MurciaUniversidad de MurciaInstituto Nacional de Investigaciones Forestales Agrícolas y PecuariasUniversidad Nacional Autónoma de MéxicoBackground: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well documented. However, there are no reports about isolation and identification of C. abortus in the colostrum or milk of infected sheep, so it is important to determine whether or not C. abortus may be present in these secretions, which are the only food of lambs. Objective: To detect C. abortus in colostrum, milk, and vaginal secretions of sheep with a history of reproductive disorders. Methods: Colostrum, milk, and vaginal exudates were collected from 66 sheep. The samples were inoculated in mouse fibroblast cell cultures and the presence of C. abortus determined by direct immunofluorescence. Results: 19 out of 66 colostrum samples (28.7%), 14 out of 66 milk samples (21.2%) and 17 out of 66 vaginal swabs (25.7%) were positive for C. abortus. The 50 samples positive for isolation and detected by immunofluorescence, together with 42 negative samples were subjected to qPCR to amplify a fragment of the ompA gene from C. abortus. Thirty-eight of the 92 samples processed by this technique were positive for C. abortus. Conclusion: The results demonstrated the presence of C. abortus in a high proportion in colostrum, milk and vaginal secretions of infected sheep. To the best of our knowledge, this is the first field study confirming the presence of C. abortus in colostrum, which shows that excretion of Chlamydia by lactogenesis could occur in the first hours after birth.https://revistas.udea.edu.co/index.php/rccp/article/view/347740chlamydia abortuschlamydial infectioncolostrumewesmilkovinesecretionsheepvaginal discharge |
| spellingShingle | María Guadalupe Martínez-Serrano Efrén Díaz-Aparicio Gabriela Palomares-Reséndiz Jorge Luis Tórtora-Pérez Hugo Ramírez-Álvarez Nieves Ortega-Hernández Jesús Salinas-Lorente José Francisco Morales-Alvarez Juan Julio César Cervantes-Morali Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep Revista Colombiana de Ciencias Pecuarias chlamydia abortus chlamydial infection colostrum ewes milk ovine secretion sheep vaginal discharge |
| title | Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep |
| title_full | Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep |
| title_fullStr | Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep |
| title_full_unstemmed | Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep |
| title_short | Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep |
| title_sort | presence of chlamydia abortus in colostrum milk and vaginal discharge samples of sheep |
| topic | chlamydia abortus chlamydial infection colostrum ewes milk ovine secretion sheep vaginal discharge |
| url | https://revistas.udea.edu.co/index.php/rccp/article/view/347740 |
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