Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
The disposal of large amounts of skin waste resulting from the blue shark fishing industry presents several industrial and environmental waste management concerns. In addition, these marine subproducts are interesting sources of collagen, a fibrous protein that shows high social and economic interes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.937036/full |
| _version_ | 1817970018885828608 |
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| author | Miguel P. Batista Miguel P. Batista Naiara Fernández Frédéric B. Gaspar Frédéric B. Gaspar Maria do Rosário Bronze Maria do Rosário Bronze Maria do Rosário Bronze Ana Rita C. Duarte |
| author_facet | Miguel P. Batista Miguel P. Batista Naiara Fernández Frédéric B. Gaspar Frédéric B. Gaspar Maria do Rosário Bronze Maria do Rosário Bronze Maria do Rosário Bronze Ana Rita C. Duarte |
| author_sort | Miguel P. Batista |
| collection | DOAJ |
| description | The disposal of large amounts of skin waste resulting from the blue shark fishing industry presents several industrial and environmental waste management concerns. In addition, these marine subproducts are interesting sources of collagen, a fibrous protein that shows high social and economic interest in a broad range of biomedical, pharmaceutical, and cosmetic applications. However, blue shark wasted skins are a poorly explored matrix for this purpose, and conventional collagen recovery methodologies involve several pre-treatment steps, long extraction times and low temperatures. This work presents a new green and sustainable collagen extraction approach using a natural deep eutectic solvent composed of citric acid:xylitol:water at a 1:1:10 molar ratio, and the chemical characterization of the extracted collagen by discontinuous electrophoresis, thermogravimetric analysis, Fourier transformed infrared spectroscopy and circular dichroism. The extracted material was a pure type I collagen, and the novel approach presented an extraction yield 2.5 times higher than the conventional one, without pre-treatment of raw material and reducing the procedure time from 96 to 1 h. Furthermore, the in vitro cytotoxicity evaluation, performed with a mouse fibroblasts cell line, has proven the biocompatibility of the extracted material. Overall, the obtained results demonstrate a simple, quick, cheap and environmentally sustainable process to obtain marine collagen with promising properties for biomedical and cosmetic applications. |
| first_indexed | 2024-04-13T20:28:51Z |
| format | Article |
| id | doaj.art-bf26837cf75f4104af1df5c14d7fbace |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-13T20:28:51Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-bf26837cf75f4104af1df5c14d7fbace2022-12-22T02:31:15ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-06-011010.3389/fchem.2022.937036937036Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic SolventsMiguel P. Batista0Miguel P. Batista1Naiara Fernández2Frédéric B. Gaspar3Frédéric B. Gaspar4Maria do Rosário Bronze5Maria do Rosário Bronze6Maria do Rosário Bronze7Ana Rita C. Duarte8iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, PortugalLAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, PortugaliBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, PortugaliBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, PortugalInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, PortugaliBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, PortugalInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, PortugalFFULisboa, Faculty of Pharmacy, University of Lisbon, Lisbon, PortugalLAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, PortugalThe disposal of large amounts of skin waste resulting from the blue shark fishing industry presents several industrial and environmental waste management concerns. In addition, these marine subproducts are interesting sources of collagen, a fibrous protein that shows high social and economic interest in a broad range of biomedical, pharmaceutical, and cosmetic applications. However, blue shark wasted skins are a poorly explored matrix for this purpose, and conventional collagen recovery methodologies involve several pre-treatment steps, long extraction times and low temperatures. This work presents a new green and sustainable collagen extraction approach using a natural deep eutectic solvent composed of citric acid:xylitol:water at a 1:1:10 molar ratio, and the chemical characterization of the extracted collagen by discontinuous electrophoresis, thermogravimetric analysis, Fourier transformed infrared spectroscopy and circular dichroism. The extracted material was a pure type I collagen, and the novel approach presented an extraction yield 2.5 times higher than the conventional one, without pre-treatment of raw material and reducing the procedure time from 96 to 1 h. Furthermore, the in vitro cytotoxicity evaluation, performed with a mouse fibroblasts cell line, has proven the biocompatibility of the extracted material. Overall, the obtained results demonstrate a simple, quick, cheap and environmentally sustainable process to obtain marine collagen with promising properties for biomedical and cosmetic applications.https://www.frontiersin.org/articles/10.3389/fchem.2022.937036/fullextraction process intensificationmarine waste valorizationnatural deep eutectic solvent (NADES)blue sharkskin collagenextract characterization |
| spellingShingle | Miguel P. Batista Miguel P. Batista Naiara Fernández Frédéric B. Gaspar Frédéric B. Gaspar Maria do Rosário Bronze Maria do Rosário Bronze Maria do Rosário Bronze Ana Rita C. Duarte Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents Frontiers in Chemistry extraction process intensification marine waste valorization natural deep eutectic solvent (NADES) blue sharkskin collagen extract characterization |
| title | Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents |
| title_full | Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents |
| title_fullStr | Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents |
| title_full_unstemmed | Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents |
| title_short | Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents |
| title_sort | extraction of biocompatible collagen from blue shark skins through the conventional extraction process intensification using natural deep eutectic solvents |
| topic | extraction process intensification marine waste valorization natural deep eutectic solvent (NADES) blue sharkskin collagen extract characterization |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.937036/full |
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