INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS
Th e aim of this study was to describe the prevalence of antibiotic resistance to some aminoglycosides, streptomycin, spectinomycin and gentamicin and three aminoglycoside- resistance genes in Escherichia coli isolated from feces and lagoon manure in six swine farms in Republic of Bulgaria. A total...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Scientific Veterinary Institute “Novi Sad”
2016-03-01
|
| Series: | Archives of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/102 |
| _version_ | 1828236049916100608 |
|---|---|
| author | Valentina Urumova Mihni Lyutskanov Vladi Petrov |
| author_facet | Valentina Urumova Mihni Lyutskanov Vladi Petrov |
| author_sort | Valentina Urumova |
| collection | DOAJ |
| description | Th e aim of this study was to describe the prevalence of antibiotic resistance to some aminoglycosides, streptomycin, spectinomycin and gentamicin
and three aminoglycoside- resistance genes in Escherichia coli isolated from feces and lagoon manure in six swine farms in Republic of Bulgaria. A
total of 274 E. coli isolates from 270 fecal samples and twelve samples from lagoon manure were tested by disk diff usion method to determine resistance patterns to 11 antimicrobial agents. Aminoglycosides resistance also was determined by E-test, agar dilution method, PCR and qPCR. Th e highest resistance observed to streptomycin (70.0%) and spectinomycin (65.5%). Multi-resistance patterns in studied E. coli strains showed that the resistance to streptomycin/spectinomycin was most frequently seen together with resistance to ampicillin, tetracycline, and sulfonamides (39.6%). The E. coli isolates resistant to streptomycin, spectinomycin were examined for the presence of strA/strB, aadA1 genes, and resistant isolates to gentamicin were evaluated for the presence of the aacC1 gene. Th e most common gene determining resistance to aminoglycosides was aadA1 which was found in 54.0% of swine isolates and lagoon manure isolates followed by straA/strB genes (32.3%). Th e aacC1gene was not identifi ed in E. coli isolates resistant to gentamicin. |
| first_indexed | 2024-04-12T20:26:16Z |
| format | Article |
| id | doaj.art-bf2a3068164f4f25bf34a7f10f306b94 |
| institution | Directory Open Access Journal |
| issn | 1820-9955 2683-4138 |
| language | English |
| last_indexed | 2024-04-12T20:26:16Z |
| publishDate | 2016-03-01 |
| publisher | Scientific Veterinary Institute “Novi Sad” |
| record_format | Article |
| series | Archives of Veterinary Medicine |
| spelling | doaj.art-bf2a3068164f4f25bf34a7f10f306b942022-12-22T03:17:52ZengScientific Veterinary Institute “Novi Sad”Archives of Veterinary Medicine1820-99552683-41382016-03-018110.46784/e-avm.v8i1.102INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLSValentina Urumova0Mihni Lyutskanov1Vladi Petrov2Trakia University, Faculty of Veterinary Medicine, Department of Microbiology, Stara Zagora, BulgariaTrakia University, Faculty of Veterinary Medicine, Department of Microbiology, Infectious and Parasitic Diseases, Stara Zagora, BulgariaTrakia University, Faculty of Veterinary Medicine, Department of Microbiology, Infectious and Parasitic Diseases, Stara Zagora, BulgariaTh e aim of this study was to describe the prevalence of antibiotic resistance to some aminoglycosides, streptomycin, spectinomycin and gentamicin and three aminoglycoside- resistance genes in Escherichia coli isolated from feces and lagoon manure in six swine farms in Republic of Bulgaria. A total of 274 E. coli isolates from 270 fecal samples and twelve samples from lagoon manure were tested by disk diff usion method to determine resistance patterns to 11 antimicrobial agents. Aminoglycosides resistance also was determined by E-test, agar dilution method, PCR and qPCR. Th e highest resistance observed to streptomycin (70.0%) and spectinomycin (65.5%). Multi-resistance patterns in studied E. coli strains showed that the resistance to streptomycin/spectinomycin was most frequently seen together with resistance to ampicillin, tetracycline, and sulfonamides (39.6%). The E. coli isolates resistant to streptomycin, spectinomycin were examined for the presence of strA/strB, aadA1 genes, and resistant isolates to gentamicin were evaluated for the presence of the aacC1 gene. Th e most common gene determining resistance to aminoglycosides was aadA1 which was found in 54.0% of swine isolates and lagoon manure isolates followed by straA/strB genes (32.3%). Th e aacC1gene was not identifi ed in E. coli isolates resistant to gentamicin.https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/102aminoglycoside resistancecommensal Escherichia colipigslagoon manure |
| spellingShingle | Valentina Urumova Mihni Lyutskanov Vladi Petrov INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS Archives of Veterinary Medicine aminoglycoside resistance commensal Escherichia coli pigs lagoon manure |
| title | INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS |
| title_full | INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS |
| title_fullStr | INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS |
| title_full_unstemmed | INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS |
| title_short | INVESTIGATIONS ON THE RESISTANCE OF COMMENSAL SWINE ESCHERICHIA COLI TO SOME AMINOGLYCOSIDES-AMINOCYCLITOLS |
| title_sort | investigations on the resistance of commensal swine escherichia coli to some aminoglycosides aminocyclitols |
| topic | aminoglycoside resistance commensal Escherichia coli pigs lagoon manure |
| url | https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/102 |
| work_keys_str_mv | AT valentinaurumova investigationsontheresistanceofcommensalswineescherichiacolitosomeaminoglycosidesaminocyclitols AT mihnilyutskanov investigationsontheresistanceofcommensalswineescherichiacolitosomeaminoglycosidesaminocyclitols AT vladipetrov investigationsontheresistanceofcommensalswineescherichiacolitosomeaminoglycosidesaminocyclitols |