Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis
Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/8/705 |
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| author | Ifrah Ismail Ali Iltaf Shah Sayed Marzouk Sherif M. Karam Asma Al Menhali |
| author_facet | Ifrah Ismail Ali Iltaf Shah Sayed Marzouk Sherif M. Karam Asma Al Menhali |
| author_sort | Ifrah Ismail Ali |
| collection | DOAJ |
| description | Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles (SDL), fed a standard diet but kept in the dark (SDD) or fed a vitamin D-deficient diet and kept in the dark (VDD). After 3 months, sera were collected to measure vitamin D levels by LC-MS/MS, gastric tissues were collected for immunohistochemical and gene expression analyses and gastric contents were collected to measure acid levels. The VDD group showed a significant decrease in the acid-secreting parietal cell-specific genes <i>Atp4a</i> and <i>Atp4b</i> when compared with the controls. This reduction was associated with an increased expression of an antral gastrin hormone. VDD gastric tissues also showed a high proliferation rate compared with SDL and SDD using an anti-BrdU antibody. This study indicates the requirement for normal vitamin D levels for proper parietal cell functions. |
| first_indexed | 2024-03-10T09:00:43Z |
| format | Article |
| id | doaj.art-bf2b4e3e307b473ea1050e5af7bd95bf |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T09:00:43Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-bf2b4e3e307b473ea1050e5af7bd95bf2023-11-22T06:49:21ZengMDPI AGBiology2079-77372021-07-0110870510.3390/biology10080705Vitamin D Is Necessary for Murine Gastric Epithelial HomeostasisIfrah Ismail Ali0Iltaf Shah1Sayed Marzouk2Sherif M. Karam3Asma Al Menhali4Department of Biology, College of Science, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al Ain 15551, United Arab EmiratesUnlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles (SDL), fed a standard diet but kept in the dark (SDD) or fed a vitamin D-deficient diet and kept in the dark (VDD). After 3 months, sera were collected to measure vitamin D levels by LC-MS/MS, gastric tissues were collected for immunohistochemical and gene expression analyses and gastric contents were collected to measure acid levels. The VDD group showed a significant decrease in the acid-secreting parietal cell-specific genes <i>Atp4a</i> and <i>Atp4b</i> when compared with the controls. This reduction was associated with an increased expression of an antral gastrin hormone. VDD gastric tissues also showed a high proliferation rate compared with SDL and SDD using an anti-BrdU antibody. This study indicates the requirement for normal vitamin D levels for proper parietal cell functions.https://www.mdpi.com/2079-7737/10/8/705vitamin D receptorvitamin D<sub>3</sub>gastric epithelial homeostasiscell proliferationcell differentiation |
| spellingShingle | Ifrah Ismail Ali Iltaf Shah Sayed Marzouk Sherif M. Karam Asma Al Menhali Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis Biology vitamin D receptor vitamin D<sub>3</sub> gastric epithelial homeostasis cell proliferation cell differentiation |
| title | Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis |
| title_full | Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis |
| title_fullStr | Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis |
| title_full_unstemmed | Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis |
| title_short | Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis |
| title_sort | vitamin d is necessary for murine gastric epithelial homeostasis |
| topic | vitamin D receptor vitamin D<sub>3</sub> gastric epithelial homeostasis cell proliferation cell differentiation |
| url | https://www.mdpi.com/2079-7737/10/8/705 |
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