Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases
Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposabl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-07-01
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| Series: | Biosensors |
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| Online Access: | http://www.mdpi.com/2079-6374/1/3/107 |
| _version_ | 1818618990227881984 |
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| author | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh |
| author_facet | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh |
| author_sort | Chung-Chiun Liu |
| collection | DOAJ |
| description | Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0–544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications. |
| first_indexed | 2024-12-16T17:30:22Z |
| format | Article |
| id | doaj.art-bf2e29d58eb94064b8a71261360238ac |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-16T17:30:22Z |
| publishDate | 2011-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-bf2e29d58eb94064b8a71261360238ac2022-12-21T22:22:56ZengMDPI AGBiosensors2079-63742011-07-011310711710.3390/bios1030107Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver DiseasesChung-Chiun LiuLiming DaiJoanne H. WangChang-Jung HsuehAlanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0–544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.http://www.mdpi.com/2079-6374/1/3/107liver diseasesalanine aminotransferaseelectrochemical detectionbiosensor |
| spellingShingle | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases Biosensors liver diseases alanine aminotransferase electrochemical detection biosensor |
| title | Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases |
| title_full | Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases |
| title_fullStr | Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases |
| title_full_unstemmed | Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases |
| title_short | Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases |
| title_sort | determination of alanine aminotransferase with an electrochemical nano ir c biosensor for the screening of liver diseases |
| topic | liver diseases alanine aminotransferase electrochemical detection biosensor |
| url | http://www.mdpi.com/2079-6374/1/3/107 |
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