Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice

Abstract Background Asthma is a worldwide common chronic airway disease that cannot be cured and results in the huge burden in public health. Oxidative stress was considered an important mechanism in the pathogenesis of asthma. Hydrogen gas been demonstrated to function as a novel antioxidant and ex...

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Main Authors: Ning Zhang, Changwen Deng, Xingxing Zhang, Jingxi Zhang, Chong Bai
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Asthma Research and Practice
Subjects:
Online Access:http://link.springer.com/article/10.1186/s40733-018-0040-y
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author Ning Zhang
Changwen Deng
Xingxing Zhang
Jingxi Zhang
Chong Bai
author_facet Ning Zhang
Changwen Deng
Xingxing Zhang
Jingxi Zhang
Chong Bai
author_sort Ning Zhang
collection DOAJ
description Abstract Background Asthma is a worldwide common chronic airway disease that cannot be cured and results in the huge burden in public health. Oxidative stress was considered an important mechanism in the pathogenesis of asthma. Hydrogen gas been demonstrated to function as a novel antioxidant and exert therapeutic antioxidant activity in a number of diseases and the function of this nontoxic gas in asthma was unclear. The purpose of the study aims to examine the effect of inhalation hydrogen gas on the pathophysiology of a mouse model of asthma. Methods A murine model of ovalbumin (OVA)-induced allergic airway inflammation was used in this study. Briefly, Mice were sensitized to ovalbumin and received inhalation of 67% high concentration of hydrogen gas for 60 min once a day for 7 consecutive days after OVA or PBS challenge respectively. Lung function was assessed in the apparatus with 4 channels of biological signal system. Morphology and goblet cell hyperplasia were stained by H/E and Periodic acid-Schiff staining. Cytologic classification in the bronchial alveolar lavage fluid (BALF) was analyzed by Wright Giemsa staining. Serum, BALF and lung tissue were collected for biochemical assay. One-way analysis of variance (ANOVA) was used to determine statistical significance between groups. Multiple comparisons were made by Bonferroni’s Multiple Comparison Test by using GraphPad Prism 5 software. Results Inhalation of hydrogen gas abrogated ovalbumin-induced the increase in lung resistance. Concomitantly, the asthmatic mice showed severe inflammatory infiltration and goblet cell hyperplasia which were reversed by hydrogen gas inhalation. Hydrogen gas inhalation reduced significantly the number of total cells, eosinophils and lymphocytes in BALF. Increased level of IL-4, IL-13, TNF-α and CXCL15 in the BALF and IL-4 in the serum were decreased significantly after inhalation. Hydrogen gas inhalation markedly upregulated the activity of decreased superoxide dismutase and significantly attenuated the increased level of malondialdehyde and myeloperoxidase. Conclusions Hydrogen gas inhalation improves lung function and protects established airway inflammation in the allergic asthmatic mice model which may be associated with the inhibition of oxidative stress process. This study provides a potential alternative therapeutic opportunity for the clinical management of asthma.
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spelling doaj.art-bf2ea46ac7bf4d82b1af0b6673387ca52022-12-22T01:50:49ZengBMCAsthma Research and Practice2054-70642018-03-01411910.1186/s40733-018-0040-yInhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic miceNing Zhang0Changwen Deng1Xingxing Zhang2Jingxi Zhang3Chong Bai4Department of Naval Aeromedicine, The Second Military Medical UniversityDepartment of Respiratory and Critical care medicine, Changhai Hospital, the Second Military Medical UniversityDepartment of Respiratory and Critical care medicine, Changhai Hospital, the Second Military Medical UniversityDepartment of Respiratory and Critical care medicine, Changhai Hospital, the Second Military Medical UniversityDepartment of Respiratory and Critical care medicine, Changhai Hospital, the Second Military Medical UniversityAbstract Background Asthma is a worldwide common chronic airway disease that cannot be cured and results in the huge burden in public health. Oxidative stress was considered an important mechanism in the pathogenesis of asthma. Hydrogen gas been demonstrated to function as a novel antioxidant and exert therapeutic antioxidant activity in a number of diseases and the function of this nontoxic gas in asthma was unclear. The purpose of the study aims to examine the effect of inhalation hydrogen gas on the pathophysiology of a mouse model of asthma. Methods A murine model of ovalbumin (OVA)-induced allergic airway inflammation was used in this study. Briefly, Mice were sensitized to ovalbumin and received inhalation of 67% high concentration of hydrogen gas for 60 min once a day for 7 consecutive days after OVA or PBS challenge respectively. Lung function was assessed in the apparatus with 4 channels of biological signal system. Morphology and goblet cell hyperplasia were stained by H/E and Periodic acid-Schiff staining. Cytologic classification in the bronchial alveolar lavage fluid (BALF) was analyzed by Wright Giemsa staining. Serum, BALF and lung tissue were collected for biochemical assay. One-way analysis of variance (ANOVA) was used to determine statistical significance between groups. Multiple comparisons were made by Bonferroni’s Multiple Comparison Test by using GraphPad Prism 5 software. Results Inhalation of hydrogen gas abrogated ovalbumin-induced the increase in lung resistance. Concomitantly, the asthmatic mice showed severe inflammatory infiltration and goblet cell hyperplasia which were reversed by hydrogen gas inhalation. Hydrogen gas inhalation reduced significantly the number of total cells, eosinophils and lymphocytes in BALF. Increased level of IL-4, IL-13, TNF-α and CXCL15 in the BALF and IL-4 in the serum were decreased significantly after inhalation. Hydrogen gas inhalation markedly upregulated the activity of decreased superoxide dismutase and significantly attenuated the increased level of malondialdehyde and myeloperoxidase. Conclusions Hydrogen gas inhalation improves lung function and protects established airway inflammation in the allergic asthmatic mice model which may be associated with the inhibition of oxidative stress process. This study provides a potential alternative therapeutic opportunity for the clinical management of asthma.http://link.springer.com/article/10.1186/s40733-018-0040-yAsthmaOxidative stressHydrogen gas inhalationCytokinePulmonary function
spellingShingle Ning Zhang
Changwen Deng
Xingxing Zhang
Jingxi Zhang
Chong Bai
Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
Asthma Research and Practice
Asthma
Oxidative stress
Hydrogen gas inhalation
Cytokine
Pulmonary function
title Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
title_full Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
title_fullStr Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
title_full_unstemmed Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
title_short Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
title_sort inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice
topic Asthma
Oxidative stress
Hydrogen gas inhalation
Cytokine
Pulmonary function
url http://link.springer.com/article/10.1186/s40733-018-0040-y
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AT changwendeng inhalationofhydrogengasattenuatesairwayinflammationandoxidativestressinallergicasthmaticmice
AT xingxingzhang inhalationofhydrogengasattenuatesairwayinflammationandoxidativestressinallergicasthmaticmice
AT jingxizhang inhalationofhydrogengasattenuatesairwayinflammationandoxidativestressinallergicasthmaticmice
AT chongbai inhalationofhydrogengasattenuatesairwayinflammationandoxidativestressinallergicasthmaticmice