Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama
The present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Mo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2006-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000400018 |
| _version_ | 1797713370882768896 |
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| author | Octavio E Sousa Franklyn Samudio Corina de Juncá José E Calzada |
| author_facet | Octavio E Sousa Franklyn Samudio Corina de Juncá José E Calzada |
| author_sort | Octavio E Sousa |
| collection | DOAJ |
| description | The present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Most of the stocks had been characterized by means of isoenzyme electrophoresis on cellulose acetate membranes. The clinical profiles of infected persons included 9 (34.6%) asymptomatic and 17 acute (65.4%) including 5 (19.2%) fatal cases, 2 under 5 years old and 3 adults. A multiplex-PCR assay based on the amplification of the non-transcribed spacer of the mini-exon gene was performed. All stocks of T. cruzi included in the study were found to correspond to Tc I group. This result supports the predominance of T. cruzi-I in the transmission cycles affecting the human population in the Republic of Panama. |
| first_indexed | 2024-03-12T07:35:30Z |
| format | Article |
| id | doaj.art-bf3385effd674b7c8b20573ebc1affe3 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:35:30Z |
| publishDate | 2006-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-bf3385effd674b7c8b20573ebc1affe32023-09-02T21:32:49ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602006-06-01101445545710.1590/S0074-02762006000400018Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in PanamaOctavio E SousaFranklyn SamudioCorina de JuncáJosé E CalzadaThe present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Most of the stocks had been characterized by means of isoenzyme electrophoresis on cellulose acetate membranes. The clinical profiles of infected persons included 9 (34.6%) asymptomatic and 17 acute (65.4%) including 5 (19.2%) fatal cases, 2 under 5 years old and 3 adults. A multiplex-PCR assay based on the amplification of the non-transcribed spacer of the mini-exon gene was performed. All stocks of T. cruzi included in the study were found to correspond to Tc I group. This result supports the predominance of T. cruzi-I in the transmission cycles affecting the human population in the Republic of Panama.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000400018Trypanosoma cruzilineageacute Chagas diseasePanama |
| spellingShingle | Octavio E Sousa Franklyn Samudio Corina de Juncá José E Calzada Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi lineage acute Chagas disease Panama |
| title | Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama |
| title_full | Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama |
| title_fullStr | Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama |
| title_full_unstemmed | Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama |
| title_short | Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama |
| title_sort | molecular characterization of human trypanosoma cruzi isolates from endemic areas in panama |
| topic | Trypanosoma cruzi lineage acute Chagas disease Panama |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000400018 |
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