Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study
Abstract Objectives The compound betamethasone is widely used to prevent scarring in dermatology. This study aims to explore the effects of compound betamethasone on vocal fold (VF) wound healing. Study design Prospective animal study in rabbits. Methods Eighteen rabbits underwent bilateral VF strip...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.1024 |
| _version_ | 1797843597558546432 |
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| author | Pengcheng Yu Chao Xue Sebastian Kiehn Rui Fang Jack J. Jiang |
| author_facet | Pengcheng Yu Chao Xue Sebastian Kiehn Rui Fang Jack J. Jiang |
| author_sort | Pengcheng Yu |
| collection | DOAJ |
| description | Abstract Objectives The compound betamethasone is widely used to prevent scarring in dermatology. This study aims to explore the effects of compound betamethasone on vocal fold (VF) wound healing. Study design Prospective animal study in rabbits. Methods Eighteen rabbits underwent bilateral VF stripping and three rabbits served as controls. 0.1 mL of compound betamethasone (1 mL: betamethasone sodium phosphate 5 mg and betamethasone dipropionate 2 mg) was injected into the right VF of each rabbit, and 0.1 mL 0.9% saline was injected into the contralateral VF. Endoscopy was performed for morphologic observation. Six larynges were harvested for histological analysis at 3 days, 7 days, and 1 month. The VFs were stained with hematoxylin and eosin (H&E), Alcian blue, and Masson' trichrome staining. Results In morphological analysis, there was no visible difference between betamethasone‐treated and saline‐treated VFs at 3 and 7 days. After 1 month, more VF scars appeared on the saline‐treated VFs than the VFs treated with betamethasone. Inflammatory cell number showed significant difference between both VFs at 3 days (p = .037) and 7 days (p = .045). No significant different was found in epithelial thickness at 1 month between the betamethasone and saline groups. The collagen in the saline‐treated VFs was significantly denser than that of the betamethasone‐treated group (p = .037). There was also a significant increase in the level of hyaluronic acid (HA) in betamethasone‐injected VFs in comparison to the saline‐injected VFs (p = .006). Conclusions Based on this study, compound betamethasone can improve VF healing. Our findings suggest that VF injection with compound betamethasone helps to minimize scarring by increasing HA level and decreasing collagen density. |
| first_indexed | 2024-04-09T17:07:13Z |
| format | Article |
| id | doaj.art-bf3543f69bb948dbb065ea0205dbda45 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-04-09T17:07:13Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-bf3543f69bb948dbb065ea0205dbda452023-04-20T11:45:42ZengWileyLaryngoscope Investigative Otolaryngology2378-80382023-04-018248849410.1002/lio2.1024Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary studyPengcheng Yu0Chao Xue1Sebastian Kiehn2Rui Fang3Jack J. Jiang4Department of Otolaryngology EENT Hospital, Fudan University Shanghai ChinaDepartment of Otolaryngology EENT Hospital, Fudan University Shanghai ChinaDepartment of Surgery, Division of Otolaryngology – Head and Neck Surgery University of Wisconsin‐Madison School of Medicine and Public Health Madison Wisconsin USADepartment of Otolaryngology EENT Hospital, Fudan University Shanghai ChinaDepartment of Otolaryngology EENT Hospital, Fudan University Shanghai ChinaAbstract Objectives The compound betamethasone is widely used to prevent scarring in dermatology. This study aims to explore the effects of compound betamethasone on vocal fold (VF) wound healing. Study design Prospective animal study in rabbits. Methods Eighteen rabbits underwent bilateral VF stripping and three rabbits served as controls. 0.1 mL of compound betamethasone (1 mL: betamethasone sodium phosphate 5 mg and betamethasone dipropionate 2 mg) was injected into the right VF of each rabbit, and 0.1 mL 0.9% saline was injected into the contralateral VF. Endoscopy was performed for morphologic observation. Six larynges were harvested for histological analysis at 3 days, 7 days, and 1 month. The VFs were stained with hematoxylin and eosin (H&E), Alcian blue, and Masson' trichrome staining. Results In morphological analysis, there was no visible difference between betamethasone‐treated and saline‐treated VFs at 3 and 7 days. After 1 month, more VF scars appeared on the saline‐treated VFs than the VFs treated with betamethasone. Inflammatory cell number showed significant difference between both VFs at 3 days (p = .037) and 7 days (p = .045). No significant different was found in epithelial thickness at 1 month between the betamethasone and saline groups. The collagen in the saline‐treated VFs was significantly denser than that of the betamethasone‐treated group (p = .037). There was also a significant increase in the level of hyaluronic acid (HA) in betamethasone‐injected VFs in comparison to the saline‐injected VFs (p = .006). Conclusions Based on this study, compound betamethasone can improve VF healing. Our findings suggest that VF injection with compound betamethasone helps to minimize scarring by increasing HA level and decreasing collagen density.https://doi.org/10.1002/lio2.1024betamethasoneinjectionvocal folds |
| spellingShingle | Pengcheng Yu Chao Xue Sebastian Kiehn Rui Fang Jack J. Jiang Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study Laryngoscope Investigative Otolaryngology betamethasone injection vocal folds |
| title | Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study |
| title_full | Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study |
| title_fullStr | Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study |
| title_full_unstemmed | Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study |
| title_short | Effects of compound betamethasone on vocal fold wound healing in rabbit model: A preliminary study |
| title_sort | effects of compound betamethasone on vocal fold wound healing in rabbit model a preliminary study |
| topic | betamethasone injection vocal folds |
| url | https://doi.org/10.1002/lio2.1024 |
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