CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment

CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment

Summary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD6...

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Bibliographic Details
Main Authors: Kuiying Ma, Xuan Wang, Linjie Wu, Lingling Yu, Jinhui Ye, Xueling Li, Lili Geng, Zhongyu Shi, Huihui Yang, Xijuan Zhang, Yongjian Zhang, Shuchang Wu, Pengfei Yuan, Yingchi Zhang, Fang Dong, Sha Hao, Linping Hu, Wensheng Wei, Riguo Fang, Tao Cheng
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:iScience
Subjects:
Cell biology
Stem cells research
Omics
Online Access:http://www.sciencedirect.com/science/article/pii/S258900422302638X
Description
Summary:Summary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD66e+ cells exhibited a 5.5-fold enrichment for functional long term HSCs compared to CD66e− cells. CD66e+CD34+CD90+CD45RA− cells displayed robust multi-lineage repopulation and serial reconstitution ability in immunodeficient mice compared to CD66e−CD34+CD90+CD45RA−cells. CD66e expression also identified almost all repopulating HSCs within the CD34+CD90+CD45RA− population. Together, these results indicated that CEACAM5 is a marker that enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment.
ISSN:2589-0042

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