CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment
Summary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD6...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422302638X |
| _version_ | 1827582021966233600 |
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| author | Kuiying Ma Xuan Wang Linjie Wu Lingling Yu Jinhui Ye Xueling Li Lili Geng Zhongyu Shi Huihui Yang Xijuan Zhang Yongjian Zhang Shuchang Wu Pengfei Yuan Yingchi Zhang Fang Dong Sha Hao Linping Hu Wensheng Wei Riguo Fang Tao Cheng |
| author_facet | Kuiying Ma Xuan Wang Linjie Wu Lingling Yu Jinhui Ye Xueling Li Lili Geng Zhongyu Shi Huihui Yang Xijuan Zhang Yongjian Zhang Shuchang Wu Pengfei Yuan Yingchi Zhang Fang Dong Sha Hao Linping Hu Wensheng Wei Riguo Fang Tao Cheng |
| author_sort | Kuiying Ma |
| collection | DOAJ |
| description | Summary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD66e+ cells exhibited a 5.5-fold enrichment for functional long term HSCs compared to CD66e− cells. CD66e+CD34+CD90+CD45RA− cells displayed robust multi-lineage repopulation and serial reconstitution ability in immunodeficient mice compared to CD66e−CD34+CD90+CD45RA−cells. CD66e expression also identified almost all repopulating HSCs within the CD34+CD90+CD45RA− population. Together, these results indicated that CEACAM5 is a marker that enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment. |
| first_indexed | 2024-03-08T22:45:33Z |
| format | Article |
| id | doaj.art-bf358c0bcb274e3fab860398a9c72498 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:45:33Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-bf358c0bcb274e3fab860398a9c724982023-12-17T06:41:11ZengElsevieriScience2589-00422023-12-012612108561CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftmentKuiying Ma0Xuan Wang1Linjie Wu2Lingling Yu3Jinhui Ye4Xueling Li5Lili Geng6Zhongyu Shi7Huihui Yang8Xijuan Zhang9Yongjian Zhang10Shuchang Wu11Pengfei Yuan12Yingchi Zhang13Fang Dong14Sha Hao15Linping Hu16Wensheng Wei17Riguo Fang18Tao Cheng19EdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaEdiGene Inc., Life Science Park, Changping District, Beijing 102206, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, ChinaState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China; Corresponding authorBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China; Corresponding authorEdiGene Inc., Life Science Park, Changping District, Beijing 102206, China; Corresponding authorState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, Tianjin 300000, China; Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, Tianjin 300000, China; Tianjin Institutes of Health Science, Tianjin 301600, China; Corresponding authorSummary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD66e+ cells exhibited a 5.5-fold enrichment for functional long term HSCs compared to CD66e− cells. CD66e+CD34+CD90+CD45RA− cells displayed robust multi-lineage repopulation and serial reconstitution ability in immunodeficient mice compared to CD66e−CD34+CD90+CD45RA−cells. CD66e expression also identified almost all repopulating HSCs within the CD34+CD90+CD45RA− population. Together, these results indicated that CEACAM5 is a marker that enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment.http://www.sciencedirect.com/science/article/pii/S258900422302638XCell biologyStem cells researchOmics |
| spellingShingle | Kuiying Ma Xuan Wang Linjie Wu Lingling Yu Jinhui Ye Xueling Li Lili Geng Zhongyu Shi Huihui Yang Xijuan Zhang Yongjian Zhang Shuchang Wu Pengfei Yuan Yingchi Zhang Fang Dong Sha Hao Linping Hu Wensheng Wei Riguo Fang Tao Cheng CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment iScience Cell biology Stem cells research Omics |
| title | CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment |
| title_full | CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment |
| title_fullStr | CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment |
| title_full_unstemmed | CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment |
| title_short | CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment |
| title_sort | cea cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long term multi lineage engraftment |
| topic | Cell biology Stem cells research Omics |
| url | http://www.sciencedirect.com/science/article/pii/S258900422302638X |
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