Identification of eleven species of the Pleuronectidae family using DNA-based techniques
Flatfish are one of the largest families in the order Pleuronectiformes and are economically important edible marine fish species. However, they have similar morphological characteristics leading to challenges in classifying correctly, which may result in mislabeling and illegal sales, such as fraud...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Korean Society of Fisheries and Aquatic Science
2023-11-01
|
| Series: | Fisheries and Aquatic Sciences |
| Subjects: | |
| Online Access: | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e60 |
| _version_ | 1797403389337796608 |
|---|---|
| author | Eun-Mi Kim Mi Nan Lee Chun-Mae Dong Eun Soo Noh Young-Ok Kim |
| author_facet | Eun-Mi Kim Mi Nan Lee Chun-Mae Dong Eun Soo Noh Young-Ok Kim |
| author_sort | Eun-Mi Kim |
| collection | DOAJ |
| description | Flatfish are one of the largest families in the order Pleuronectiformes and are economically important edible marine fish species. However, they have similar morphological characteristics leading to challenges in classifying correctly, which may result in mislabeling and illegal sales, such as fraudulent labeling of processed food. Therefore, accurate identification is important to ensure the quality and safety of domestic markets in Korea. Species-specific primers were prepared from the mainly consumed eleven species of the order Pleuronectiformes. To rapidly identify the 11 flatfish species, a highly efficient, rapid, multiplex polymerase chain reaction (PCR) with species-specific primers was developed. Species-specific primer sets were designed for the mitochondrial DNA cytochrome c oxidase subunit I gene. Species-specific multiplex PCR (MSS-PCR) either specifically amplified a PCR product of a unique size or failed. This MSS-PCR analysis is easy to perform and yields reliable results in less time than the previous Sanger sequencing methods. This technique could be a powerful tool for the identification of the 11 species b the family Pleuronectidae and can contribute to the prevention of falsified labeling and protection of consumer rights. |
| first_indexed | 2024-03-09T02:38:42Z |
| format | Article |
| id | doaj.art-bf3697de5eb64b189b424a629e0c875e |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-03-09T02:38:42Z |
| publishDate | 2023-11-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-bf3697de5eb64b189b424a629e0c875e2023-12-06T08:00:24ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572023-11-01261167868810.47853/FAS.2023.e60Identification of eleven species of the Pleuronectidae family using DNA-based techniquesEun-Mi Kim0Mi Nan Lee1Chun-Mae Dong2Eun Soo Noh3Young-Ok Kim4Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaBiotechnology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaBiotechnology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaBiotechnology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaBiotechnology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaFlatfish are one of the largest families in the order Pleuronectiformes and are economically important edible marine fish species. However, they have similar morphological characteristics leading to challenges in classifying correctly, which may result in mislabeling and illegal sales, such as fraudulent labeling of processed food. Therefore, accurate identification is important to ensure the quality and safety of domestic markets in Korea. Species-specific primers were prepared from the mainly consumed eleven species of the order Pleuronectiformes. To rapidly identify the 11 flatfish species, a highly efficient, rapid, multiplex polymerase chain reaction (PCR) with species-specific primers was developed. Species-specific primer sets were designed for the mitochondrial DNA cytochrome c oxidase subunit I gene. Species-specific multiplex PCR (MSS-PCR) either specifically amplified a PCR product of a unique size or failed. This MSS-PCR analysis is easy to perform and yields reliable results in less time than the previous Sanger sequencing methods. This technique could be a powerful tool for the identification of the 11 species b the family Pleuronectidae and can contribute to the prevention of falsified labeling and protection of consumer rights. http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e60Pleuronectidae familyMitochondrial DNACytochrome c oxidase subunit I gene (COI) identificationPolymerase chain reaction (PCR)Multiplex species-specific |
| spellingShingle | Eun-Mi Kim Mi Nan Lee Chun-Mae Dong Eun Soo Noh Young-Ok Kim Identification of eleven species of the Pleuronectidae family using DNA-based techniques Fisheries and Aquatic Sciences Pleuronectidae family Mitochondrial DNA Cytochrome c oxidase subunit I gene (COI) identification Polymerase chain reaction (PCR) Multiplex species-specific |
| title | Identification of eleven species of the Pleuronectidae family using DNA-based techniques |
| title_full | Identification of eleven species of the Pleuronectidae family using DNA-based techniques |
| title_fullStr | Identification of eleven species of the Pleuronectidae family using DNA-based techniques |
| title_full_unstemmed | Identification of eleven species of the Pleuronectidae family using DNA-based techniques |
| title_short | Identification of eleven species of the Pleuronectidae family using DNA-based techniques |
| title_sort | identification of eleven species of the pleuronectidae family using dna based techniques |
| topic | Pleuronectidae family Mitochondrial DNA Cytochrome c oxidase subunit I gene (COI) identification Polymerase chain reaction (PCR) Multiplex species-specific |
| url | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2023.e60 |
| work_keys_str_mv | AT eunmikim identificationofelevenspeciesofthepleuronectidaefamilyusingdnabasedtechniques AT minanlee identificationofelevenspeciesofthepleuronectidaefamilyusingdnabasedtechniques AT chunmaedong identificationofelevenspeciesofthepleuronectidaefamilyusingdnabasedtechniques AT eunsoonoh identificationofelevenspeciesofthepleuronectidaefamilyusingdnabasedtechniques AT youngokkim identificationofelevenspeciesofthepleuronectidaefamilyusingdnabasedtechniques |