电针通过调节2型糖尿病小鼠肠道菌群降低血糖
Abstract Background The development of diabetes is closely related to the gut microbiota in recent studies, which can be influenced by intestinal motility. A few studies report that electroacupuncture (EA) can lower blood glucose. EA can promote colonic motility and influence gut microbes. In this s...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
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| Series: | Journal of Diabetes |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1753-0407.13323 |
| _version_ | 1811185760050610176 |
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| author | Jing An Lingli Wang Shuangning Song Lugao Tian Qingqing Liu Minhui Mei Wenhua Li Shi Liu |
| author_facet | Jing An Lingli Wang Shuangning Song Lugao Tian Qingqing Liu Minhui Mei Wenhua Li Shi Liu |
| author_sort | Jing An |
| collection | DOAJ |
| description | Abstract Background The development of diabetes is closely related to the gut microbiota in recent studies, which can be influenced by intestinal motility. A few studies report that electroacupuncture (EA) can lower blood glucose. EA can promote colonic motility and influence gut microbes. In this study, we explored the effect of the EA on blood glucose level in mice with type 2 diabetes (T2D) and its mechanism. Methods The T2D mice model, fecal microbiota transplantation mice model, and KitW/Wv mice model （Point mutation of mouse W locus encoding kit gene）were used to investigate the effect of EA on blood glucose as well as the mechanism; The blood glucose and insulin resistance level and the intestinal flora were evaluated. The level of intestinal junction protein, inflammatory cytokines in the serum, interstitial cells of Cajal content, and colonic motility were detected. Lastly, the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT pathway was explored. Results EA lowered the blood glucose level, altered the gut microbiota, and promoted colonic motility in T2D mice. EA‐altered microbiota decreased the blood glucose level and insulin resistance in the antibiotics‐treated diabetic mice. EA increased tight junction protein, lowered inflammatory factors, and regulated the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT pathway in the liver and muscles. EA could not reduce the blood glucose and regulated gut microbiota in the KitW/Wv mice model. Conclusions EA promoted intestinal motility to regulate the intestinal flora, thereby reducing the level of systemic inflammation, and ultimately lowering the blood glucose by the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT signal pathway. |
| first_indexed | 2024-04-11T13:34:40Z |
| format | Article |
| id | doaj.art-bf388975a9cf4464a7d16eb3edba9919 |
| institution | Directory Open Access Journal |
| issn | 1753-0393 1753-0407 |
| language | English |
| last_indexed | 2024-04-11T13:34:40Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes |
| spelling | doaj.art-bf388975a9cf4464a7d16eb3edba99192022-12-22T04:21:37ZengWileyJournal of Diabetes1753-03931753-04072022-10-01141069571010.1111/1753-0407.13323电针通过调节2型糖尿病小鼠肠道菌群降低血糖Jing An0Lingli Wang1Shuangning Song2Lugao Tian3Qingqing Liu4Minhui Mei5Wenhua Li6Shi Liu7Department of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaAbstract Background The development of diabetes is closely related to the gut microbiota in recent studies, which can be influenced by intestinal motility. A few studies report that electroacupuncture (EA) can lower blood glucose. EA can promote colonic motility and influence gut microbes. In this study, we explored the effect of the EA on blood glucose level in mice with type 2 diabetes (T2D) and its mechanism. Methods The T2D mice model, fecal microbiota transplantation mice model, and KitW/Wv mice model （Point mutation of mouse W locus encoding kit gene）were used to investigate the effect of EA on blood glucose as well as the mechanism; The blood glucose and insulin resistance level and the intestinal flora were evaluated. The level of intestinal junction protein, inflammatory cytokines in the serum, interstitial cells of Cajal content, and colonic motility were detected. Lastly, the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT pathway was explored. Results EA lowered the blood glucose level, altered the gut microbiota, and promoted colonic motility in T2D mice. EA‐altered microbiota decreased the blood glucose level and insulin resistance in the antibiotics‐treated diabetic mice. EA increased tight junction protein, lowered inflammatory factors, and regulated the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT pathway in the liver and muscles. EA could not reduce the blood glucose and regulated gut microbiota in the KitW/Wv mice model. Conclusions EA promoted intestinal motility to regulate the intestinal flora, thereby reducing the level of systemic inflammation, and ultimately lowering the blood glucose by the IKKβ/NF‐κB‐JNK‐IRS‐1‐AKT signal pathway.https://doi.org/10.1111/1753-0407.13323肠道菌群2型糖尿病电针炎症ICC |
| spellingShingle | Jing An Lingli Wang Shuangning Song Lugao Tian Qingqing Liu Minhui Mei Wenhua Li Shi Liu 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 Journal of Diabetes 肠道菌群 2型糖尿病 电针 炎症 ICC |
| title | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| title_full | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| title_fullStr | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| title_full_unstemmed | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| title_short | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| title_sort | 电针通过调节2型糖尿病小鼠肠道菌群降低血糖 |
| topic | 肠道菌群 2型糖尿病 电针 炎症 ICC |
| url | https://doi.org/10.1111/1753-0407.13323 |
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