Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions
Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-02-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671113001811 |
| _version_ | 1818545331895271424 |
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| author | Caroline Kubaczka Claire Senner Marcos J. Araúzo-Bravo Neha Sharma Peter Kuckenberg Astrid Becker Andreas Zimmer Oliver Brüstle Michael Peitz Myriam Hemberger Hubert Schorle |
| author_facet | Caroline Kubaczka Claire Senner Marcos J. Araúzo-Bravo Neha Sharma Peter Kuckenberg Astrid Becker Andreas Zimmer Oliver Brüstle Michael Peitz Myriam Hemberger Hubert Schorle |
| author_sort | Caroline Kubaczka |
| collection | DOAJ |
| description | Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic lineage. |
| first_indexed | 2024-12-12T07:38:54Z |
| format | Article |
| id | doaj.art-bf3b7ad7543542b0b80dc969a20137d6 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-12T07:38:54Z |
| publishDate | 2014-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-bf3b7ad7543542b0b80dc969a20137d62022-12-22T00:32:51ZengElsevierStem Cell Reports2213-67112014-02-012223224210.1016/j.stemcr.2013.12.013Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined ConditionsCaroline Kubaczka0Claire Senner1Marcos J. Araúzo-Bravo2Neha Sharma3Peter Kuckenberg4Astrid Becker5Andreas Zimmer6Oliver Brüstle7Michael Peitz8Myriam Hemberger9Hubert Schorle10Department of Developmental Pathology, Institute of Pathology, University of Bonn, 53127 Bonn, GermanyEpigenetics Programme, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UKMax Planck Institute for Molecular Biomedicine, Group Laboratory of Computational Biology and Bioinformatics, 48149 Münster, GermanyDepartment of Developmental Pathology, Institute of Pathology, University of Bonn, 53127 Bonn, GermanyDepartment of Developmental Pathology, Institute of Pathology, University of Bonn, 53127 Bonn, GermanyLife and Brain Center, Institute of Molecular Psychiatry, University of Bonn, 53127 Bonn, GermanyLife and Brain Center, Institute of Molecular Psychiatry, University of Bonn, 53127 Bonn, GermanyLife and Brain Center, Institute of Reconstructive Neurobiology, University of Bonn and Hertie Foundation, 53127 Bonn, GermanyLife and Brain Center, Institute of Reconstructive Neurobiology, University of Bonn and Hertie Foundation, 53127 Bonn, GermanyEpigenetics Programme, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UKDepartment of Developmental Pathology, Institute of Pathology, University of Bonn, 53127 Bonn, GermanyTrophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic lineage.http://www.sciencedirect.com/science/article/pii/S2213671113001811 |
| spellingShingle | Caroline Kubaczka Claire Senner Marcos J. Araúzo-Bravo Neha Sharma Peter Kuckenberg Astrid Becker Andreas Zimmer Oliver Brüstle Michael Peitz Myriam Hemberger Hubert Schorle Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions Stem Cell Reports |
| title | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
| title_full | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
| title_fullStr | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
| title_full_unstemmed | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
| title_short | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
| title_sort | derivation and maintenance of murine trophoblast stem cells under defined conditions |
| url | http://www.sciencedirect.com/science/article/pii/S2213671113001811 |
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