Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius
In order to study the extraction technology of total flavonoids from Phellinus igniarius systematically, clarify the composition and content of flavonoids in total flavonoids, and explore the biological activity of total flavonoids from Phellinus igniarius. The extraction technology of Phellinus ign...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2024-04-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050290 |
| _version_ | 1797249036211716096 |
|---|---|
| author | Tingwei FU Ziwei ZHOU Ying SHAO Peng ZHANG Xiaoya NING Yang YANG Anhui CHEN |
| author_facet | Tingwei FU Ziwei ZHOU Ying SHAO Peng ZHANG Xiaoya NING Yang YANG Anhui CHEN |
| author_sort | Tingwei FU |
| collection | DOAJ |
| description | In order to study the extraction technology of total flavonoids from Phellinus igniarius systematically, clarify the composition and content of flavonoids in total flavonoids, and explore the biological activity of total flavonoids from Phellinus igniarius. The extraction technology of Phellinus igniarius total flavonoids was optimized by response surface method, the composition of Phellinus igniarius total flavonoids was analyzed, and some of its biological activities were also detected. The results showed that the optimal extraction conditions were extraction temperature of 73 ℃, solid-liquid ratio of 1:50 g/mL and extraction time of 3 h, under which the yield of Phellinus igniarius total flavonoids reached 2.68%. The types and contents of flavonoids in Phellinus igniarius total flavonoids were determined by HPLC. Total flavonoids of Phellinus igniarius mainly contained taxifolin, quercetin and kaempferol, and the content of taxifolin was 3727.31 mg/kg. The analysis of biological activities of Phellinus igniarius total flavonoids showed that it had certain antioxidant, lipid-lowering and hypoglycemic activities in vitro, and the scavenging rate of DPPH free radicals reached 58.63%±0.45% when its concentration was 14 μg/mL, and the scavenging rate of hydroxyl free radicals reached 52.51%±1.49% when its concentration was 0.08 mg/mL. The experimental results of lipid-lowering activity showed that the inhibitory rate of Phellinus igniarius total flavonoids on pancreatic lipase reached 10.56%±0.06% at the concentration of 6 mg/mL, and the inhibitory rate on cholesterol micelle solubility reached 32.59%±0.78% at the concentration of 4 mg/mL. Phellinus igniarius total flavonids had obvious hypoglycemic activity, and its IC50 for α-glucosidase and α-amylase was 71.42 mg/mL and 97.28 mg/mL respectively. |
| first_indexed | 2024-04-24T20:24:06Z |
| format | Article |
| id | doaj.art-bf3b7d3ecb0a4896a21b4f6fbdcbe372 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-24T20:24:06Z |
| publishDate | 2024-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-bf3b7d3ecb0a4896a21b4f6fbdcbe3722024-03-22T06:45:48ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-04-0145719120010.13386/j.issn1002-0306.20230502902023050290-7Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniariusTingwei FU0Ziwei ZHOU1Ying SHAO2Peng ZHANG3Xiaoya NING4Yang YANG5Anhui CHEN6College of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaCollege of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaCollege of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaXuzhou Tianyi Food Research Institute Company Limited, Xuzhou 210009, ChinaCollege of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaCollege of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaCollege of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, ChinaIn order to study the extraction technology of total flavonoids from Phellinus igniarius systematically, clarify the composition and content of flavonoids in total flavonoids, and explore the biological activity of total flavonoids from Phellinus igniarius. The extraction technology of Phellinus igniarius total flavonoids was optimized by response surface method, the composition of Phellinus igniarius total flavonoids was analyzed, and some of its biological activities were also detected. The results showed that the optimal extraction conditions were extraction temperature of 73 ℃, solid-liquid ratio of 1:50 g/mL and extraction time of 3 h, under which the yield of Phellinus igniarius total flavonoids reached 2.68%. The types and contents of flavonoids in Phellinus igniarius total flavonoids were determined by HPLC. Total flavonoids of Phellinus igniarius mainly contained taxifolin, quercetin and kaempferol, and the content of taxifolin was 3727.31 mg/kg. The analysis of biological activities of Phellinus igniarius total flavonoids showed that it had certain antioxidant, lipid-lowering and hypoglycemic activities in vitro, and the scavenging rate of DPPH free radicals reached 58.63%±0.45% when its concentration was 14 μg/mL, and the scavenging rate of hydroxyl free radicals reached 52.51%±1.49% when its concentration was 0.08 mg/mL. The experimental results of lipid-lowering activity showed that the inhibitory rate of Phellinus igniarius total flavonoids on pancreatic lipase reached 10.56%±0.06% at the concentration of 6 mg/mL, and the inhibitory rate on cholesterol micelle solubility reached 32.59%±0.78% at the concentration of 4 mg/mL. Phellinus igniarius total flavonids had obvious hypoglycemic activity, and its IC50 for α-glucosidase and α-amylase was 71.42 mg/mL and 97.28 mg/mL respectively.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050290phellinus igniariustotal foavonoidsextractioncomponent analysisbiological activity |
| spellingShingle | Tingwei FU Ziwei ZHOU Ying SHAO Peng ZHANG Xiaoya NING Yang YANG Anhui CHEN Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius Shipin gongye ke-ji phellinus igniarius total foavonoids extraction component analysis biological activity |
| title | Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius |
| title_full | Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius |
| title_fullStr | Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius |
| title_full_unstemmed | Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius |
| title_short | Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius |
| title_sort | extraction component analysis and biological activity evaluation of total flavonoids from phellinus igniarius |
| topic | phellinus igniarius total foavonoids extraction component analysis biological activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050290 |
| work_keys_str_mv | AT tingweifu extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT ziweizhou extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT yingshao extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT pengzhang extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT xiaoyaning extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT yangyang extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius AT anhuichen extractioncomponentanalysisandbiologicalactivityevaluationoftotalflavonoidsfromphellinusigniarius |