Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18
High-Risk Human Papillomavirus (HR-HPV) types 16 and 18 are estimated to be responsible for 72.4% of all HPV-related cancers worldwide in both men and women, including cervical, anal, penile, vulval, vaginal and head and neck cancers [1]. Important efforts worldwide have devoted to the study of thes...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923001336 |
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| author | Nuvia Kantún-Moreno Guadalupe Ayora-Talavera María del Refugio González-Losa Jesús Gómez-Carballo Laura Conde-Ferráez |
| author_facet | Nuvia Kantún-Moreno Guadalupe Ayora-Talavera María del Refugio González-Losa Jesús Gómez-Carballo Laura Conde-Ferráez |
| author_sort | Nuvia Kantún-Moreno |
| collection | DOAJ |
| description | High-Risk Human Papillomavirus (HR-HPV) types 16 and 18 are estimated to be responsible for 72.4% of all HPV-related cancers worldwide in both men and women, including cervical, anal, penile, vulval, vaginal and head and neck cancers [1]. Important efforts worldwide have devoted to the study of these genotypes, throughout epidemiology and basic science approaches. Of particular interest are the genes from the early region (E), coding non-structural proteins. Early genes E1 and E2 products are involved in replication and transcription regulation, while E6 and E7 proteins are recognised for their oncogenic potential. In this data report, we described a set of primers based on reference sequences from HPV16 and HPV18 designed to cover the early region of these oncogenic genotypes. The design was based on multiple sequences alignment to identify the less conserved regions along the open reading frames (ORFs) E6, E7, E1 and E2. The design allows a highly stringent real time PCR essay ranged from 123 to 598 bp overlapping products for HPV16 (12 products in total) and from 183 to 526 bp for HPV18 (11 products in total), both spanning the early genomic region. The high annealing temperatures (Ta) and regions selected for primer bind were intended for genotypic specificity, without compromising the qPCR amplification efficiency (≥ 90%). Evaluation of qPCR conditions for primer set was performed using DNA standards as controls, generated from the HPV16 and 18 genomes clones. This provides relevant information for further multiple quantitative real-time PCR analysis (qPCR), using the SYBR green chemistry, which is is more affordable than generating multiple fluorescently labeled probes. |
| first_indexed | 2024-04-09T23:44:06Z |
| format | Article |
| id | doaj.art-bf3c9b59484744219f94d9340817c8cd |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-09T23:44:06Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-bf3c9b59484744219f94d9340817c8cd2023-03-18T04:41:59ZengElsevierData in Brief2352-34092023-04-0147109015Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18Nuvia Kantún-Moreno0Guadalupe Ayora-Talavera1María del Refugio González-Losa2Jesús Gómez-Carballo3Laura Conde-Ferráez4Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Laboratorio de Virología. Merida, Yucatan, MexicoUniversidad Autónoma de Yucatán, Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Laboratorio de Virología. Merida, Yucatan, MexicoUniversidad Autónoma de Yucatán, Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Laboratorio de Virología. Merida, Yucatan, MexicoUniversidad Autónoma de Yucatán, Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Laboratorio de Virología. Merida, Yucatan, MexicoCorresponding author.; Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales, Dr. Hideyo Noguchi, Laboratorio de Virología. Merida, Yucatan, MexicoHigh-Risk Human Papillomavirus (HR-HPV) types 16 and 18 are estimated to be responsible for 72.4% of all HPV-related cancers worldwide in both men and women, including cervical, anal, penile, vulval, vaginal and head and neck cancers [1]. Important efforts worldwide have devoted to the study of these genotypes, throughout epidemiology and basic science approaches. Of particular interest are the genes from the early region (E), coding non-structural proteins. Early genes E1 and E2 products are involved in replication and transcription regulation, while E6 and E7 proteins are recognised for their oncogenic potential. In this data report, we described a set of primers based on reference sequences from HPV16 and HPV18 designed to cover the early region of these oncogenic genotypes. The design was based on multiple sequences alignment to identify the less conserved regions along the open reading frames (ORFs) E6, E7, E1 and E2. The design allows a highly stringent real time PCR essay ranged from 123 to 598 bp overlapping products for HPV16 (12 products in total) and from 183 to 526 bp for HPV18 (11 products in total), both spanning the early genomic region. The high annealing temperatures (Ta) and regions selected for primer bind were intended for genotypic specificity, without compromising the qPCR amplification efficiency (≥ 90%). Evaluation of qPCR conditions for primer set was performed using DNA standards as controls, generated from the HPV16 and 18 genomes clones. This provides relevant information for further multiple quantitative real-time PCR analysis (qPCR), using the SYBR green chemistry, which is is more affordable than generating multiple fluorescently labeled probes.http://www.sciencedirect.com/science/article/pii/S2352340923001336HPVGenomeqPCRPrimersMelt curve |
| spellingShingle | Nuvia Kantún-Moreno Guadalupe Ayora-Talavera María del Refugio González-Losa Jesús Gómez-Carballo Laura Conde-Ferráez Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 Data in Brief HPV Genome qPCR Primers Melt curve |
| title | Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 |
| title_full | Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 |
| title_fullStr | Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 |
| title_full_unstemmed | Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 |
| title_short | Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18 |
| title_sort | design of a data set of qpcr primers for the early region of human papillomavirus oncogenic types 16 and 18 |
| topic | HPV Genome qPCR Primers Melt curve |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923001336 |
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