An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings
Arsenic (As) contamination of the rice agro-ecosystem is a major concern for rice farmers of South East Asia as it imposes a serious threat to human and animal life; thus, there is an unrelenting need to explore the ways by which arsenic stress mitigation could be achieved. In the present investigat...
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MDPI AG
2022-07-01
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| Series: | Antioxidants |
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| author | Shuvasish Choudhury Debojyoti Moulick Muhammed Khairujjaman Mazumder Binaya Kumar Pattnaik Dibakar Ghosh Lakshminarayana R. Vemireddy Adil Aldhahrani Mohamed Mohamed Soliman Ahmed Gaber Akbar Hossain |
| author_facet | Shuvasish Choudhury Debojyoti Moulick Muhammed Khairujjaman Mazumder Binaya Kumar Pattnaik Dibakar Ghosh Lakshminarayana R. Vemireddy Adil Aldhahrani Mohamed Mohamed Soliman Ahmed Gaber Akbar Hossain |
| author_sort | Shuvasish Choudhury |
| collection | DOAJ |
| description | Arsenic (As) contamination of the rice agro-ecosystem is a major concern for rice farmers of South East Asia as it imposes a serious threat to human and animal life; thus, there is an unrelenting need to explore the ways by which arsenic stress mitigation could be achieved. In the present investigation, we explore the effect of zinc (Zn<sup>2+</sup>) supplementation using the seed priming technique for the mitigation of As-induced stress responses in developing rice seedlings. In addition to the physiological and biochemical attributes, we also studied the interactive effect of Zn<sup>2+</sup> in regulating As-induced changes by targeting antioxidant enzymes using a computational approach. Our findings suggest that Zn<sup>2+</sup> and As can effectively modulate redox homeostasis by limiting ROS production and thereby confer protection against oxidative stress. The results also show that As had a significant impact on seedling growth, which was restored by Zn<sup>2+</sup> and also minimized the As uptake. A remarkable outcome of the present investigation is that the varietal difference was significant in determining the efficacy of the Zn<sup>2+</sup> priming. Further, based on the findings of computational studies, we observed differences in the surface overlap of the antioxidant target enzymes of rice, indicating that the Zn<sup>2+</sup> might have foiled the interaction of As with the enzymes. This is undoubtedly a fascinating approach that interprets the mode of action of the antioxidative enzymes under the metal/metalloid-tempted stress condition in rice by pointing at designated targets. The results of the current investigation are rationally significant and may be the pioneering beginning of an exciting and useful method of integrating physiological and biochemical analysis together with a computational modelling approach for evaluating the stress modulating effects of Zn<sup>2+</sup> seed priming on As-induced responses in developing rice seedlings. |
| first_indexed | 2024-03-09T11:58:58Z |
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| id | doaj.art-bf3fab1df72b4ea98ce38cd513d9ae55 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:58:58Z |
| publishDate | 2022-07-01 |
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| series | Antioxidants |
| spelling | doaj.art-bf3fab1df72b4ea98ce38cd513d9ae552023-11-30T23:05:59ZengMDPI AGAntioxidants2076-39212022-07-01118150010.3390/antiox11081500An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice SeedlingsShuvasish Choudhury0Debojyoti Moulick1Muhammed Khairujjaman Mazumder2Binaya Kumar Pattnaik3Dibakar Ghosh4Lakshminarayana R. Vemireddy5Adil Aldhahrani6Mohamed Mohamed Soliman7Ahmed Gaber8Akbar Hossain9Plant Stress Biology and Metabolomics Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar 788011, IndiaPlant Stress Biology and Metabolomics Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar 788011, IndiaPlant Stress Biology and Metabolomics Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar 788011, IndiaSymbiosis Institute of Geoinformatics, Symbiosis International (Deemed University), Pune 411016, IndiaDivision of Agronomy, ICAR—Indian Institute of Water Management, Chandrashekarpur, Bhubaneshwar 751023, IndiaDepartment of Molecular Biology and Biotechnology, Sri Venkateswara Agricultural College, Acharya NG Ranga Agricultural University, Tirupati 517502, IndiaClinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi ArabiaClinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi ArabiaDepartment of Biology, College of Science, Taif University, Taif 21944, Saudi ArabiaDepartment of Agronomy, Bangladesh Wheat and Maize Research Institute, Dinajpur 5200, BangladeshArsenic (As) contamination of the rice agro-ecosystem is a major concern for rice farmers of South East Asia as it imposes a serious threat to human and animal life; thus, there is an unrelenting need to explore the ways by which arsenic stress mitigation could be achieved. In the present investigation, we explore the effect of zinc (Zn<sup>2+</sup>) supplementation using the seed priming technique for the mitigation of As-induced stress responses in developing rice seedlings. In addition to the physiological and biochemical attributes, we also studied the interactive effect of Zn<sup>2+</sup> in regulating As-induced changes by targeting antioxidant enzymes using a computational approach. Our findings suggest that Zn<sup>2+</sup> and As can effectively modulate redox homeostasis by limiting ROS production and thereby confer protection against oxidative stress. The results also show that As had a significant impact on seedling growth, which was restored by Zn<sup>2+</sup> and also minimized the As uptake. A remarkable outcome of the present investigation is that the varietal difference was significant in determining the efficacy of the Zn<sup>2+</sup> priming. Further, based on the findings of computational studies, we observed differences in the surface overlap of the antioxidant target enzymes of rice, indicating that the Zn<sup>2+</sup> might have foiled the interaction of As with the enzymes. This is undoubtedly a fascinating approach that interprets the mode of action of the antioxidative enzymes under the metal/metalloid-tempted stress condition in rice by pointing at designated targets. The results of the current investigation are rationally significant and may be the pioneering beginning of an exciting and useful method of integrating physiological and biochemical analysis together with a computational modelling approach for evaluating the stress modulating effects of Zn<sup>2+</sup> seed priming on As-induced responses in developing rice seedlings.https://www.mdpi.com/2076-3921/11/8/1500Zn x Asricemolecular dockingoxidative stressROS |
| spellingShingle | Shuvasish Choudhury Debojyoti Moulick Muhammed Khairujjaman Mazumder Binaya Kumar Pattnaik Dibakar Ghosh Lakshminarayana R. Vemireddy Adil Aldhahrani Mohamed Mohamed Soliman Ahmed Gaber Akbar Hossain An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings Antioxidants Zn x As rice molecular docking oxidative stress ROS |
| title | An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings |
| title_full | An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings |
| title_fullStr | An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings |
| title_full_unstemmed | An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings |
| title_short | An In Vitro and In Silico Perspective Study of Seed Priming with Zinc on the Phytotoxicity and Accumulation Pattern of Arsenic in Rice Seedlings |
| title_sort | in vitro and in silico perspective study of seed priming with zinc on the phytotoxicity and accumulation pattern of arsenic in rice seedlings |
| topic | Zn x As rice molecular docking oxidative stress ROS |
| url | https://www.mdpi.com/2076-3921/11/8/1500 |
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