Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms
We used metagenomic sequencing combined with morphological and chemical analyses to investigate microbial taxa and functions related to copper-resistance and microbiologically influenced corrosion in mature copper-associated biofilms in coastal seawater for 44 months. Facultative anaerobic microbes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02863/full |
| _version_ | 1811295191479353344 |
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| author | Yimeng Zhang Yimeng Zhang Yimeng Zhang Yimeng Zhang Yan Ma Yan Ma Yan Ma Ruiyong Zhang Binbin Zhang Binbin Zhang Binbin Zhang Xiaofan Zhai Xiaofan Zhai Xiaofan Zhai Wangqiang Li Wangqiang Li Wangqiang Li Liting Xu Liting Xu Liting Xu Quantong Jiang Quantong Jiang Quantong Jiang Jizhou Duan Jizhou Duan Jizhou Duan Baorong Hou Baorong Hou Baorong Hou |
| author_facet | Yimeng Zhang Yimeng Zhang Yimeng Zhang Yimeng Zhang Yan Ma Yan Ma Yan Ma Ruiyong Zhang Binbin Zhang Binbin Zhang Binbin Zhang Xiaofan Zhai Xiaofan Zhai Xiaofan Zhai Wangqiang Li Wangqiang Li Wangqiang Li Liting Xu Liting Xu Liting Xu Quantong Jiang Quantong Jiang Quantong Jiang Jizhou Duan Jizhou Duan Jizhou Duan Baorong Hou Baorong Hou Baorong Hou |
| author_sort | Yimeng Zhang |
| collection | DOAJ |
| description | We used metagenomic sequencing combined with morphological and chemical analyses to investigate microbial taxa and functions related to copper-resistance and microbiologically influenced corrosion in mature copper-associated biofilms in coastal seawater for 44 months. Facultative anaerobic microbes such as Woeseia sp. were found to be the dominant groups on the copper surface. Genes related to stress response and possible heavy metal transport systems, especially RNA polymerase sigma factors (rpoE) and putative ATP-binding cassette (ABC) transport system permease protein (ABC.CD.P) were observed to be highly enriched in copper-associated biofilms, while genes encoding DNA-methyltransferase and RNA polymerase subunit were highly enriched in aluminum-associated biofilms and seawater planktonic cells, respectively. Moreover, copper-associated biofilms harbored abundant copper-resistance genes including cus, cop and pco, as well as abundant genes related to extracellular polymeric substances, indicating the presence of diverse copper-resistance patterns. The proportion of dsr in copper-associated biofilms, key genes related to sulfide production, was as low as that in aluminum biofilm and seawater, which ruled out the possibility of microbial sulfide-induced copper-corrosion under field conditions. These results may fill knowledge gaps about the in situ microbial functions of marine biofilms and their effects on toxic-metal corrosion. |
| first_indexed | 2024-04-13T05:28:51Z |
| format | Article |
| id | doaj.art-bf411274a837436a8120047e6d01da58 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T05:28:51Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bf411274a837436a8120047e6d01da582022-12-22T03:00:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-12-011010.3389/fmicb.2019.02863485988Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine BiofilmsYimeng Zhang0Yimeng Zhang1Yimeng Zhang2Yimeng Zhang3Yan Ma4Yan Ma5Yan Ma6Ruiyong Zhang7Binbin Zhang8Binbin Zhang9Binbin Zhang10Xiaofan Zhai11Xiaofan Zhai12Xiaofan Zhai13Wangqiang Li14Wangqiang Li15Wangqiang Li16Liting Xu17Liting Xu18Liting Xu19Quantong Jiang20Quantong Jiang21Quantong Jiang22Jizhou Duan23Jizhou Duan24Jizhou Duan25Baorong Hou26Baorong Hou27Baorong Hou28Key Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaFederal Institute for Geosciences and Natural Resources, Hanover, GermanyKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Environmental Corrosion and Biofouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaOpen Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaWe used metagenomic sequencing combined with morphological and chemical analyses to investigate microbial taxa and functions related to copper-resistance and microbiologically influenced corrosion in mature copper-associated biofilms in coastal seawater for 44 months. Facultative anaerobic microbes such as Woeseia sp. were found to be the dominant groups on the copper surface. Genes related to stress response and possible heavy metal transport systems, especially RNA polymerase sigma factors (rpoE) and putative ATP-binding cassette (ABC) transport system permease protein (ABC.CD.P) were observed to be highly enriched in copper-associated biofilms, while genes encoding DNA-methyltransferase and RNA polymerase subunit were highly enriched in aluminum-associated biofilms and seawater planktonic cells, respectively. Moreover, copper-associated biofilms harbored abundant copper-resistance genes including cus, cop and pco, as well as abundant genes related to extracellular polymeric substances, indicating the presence of diverse copper-resistance patterns. The proportion of dsr in copper-associated biofilms, key genes related to sulfide production, was as low as that in aluminum biofilm and seawater, which ruled out the possibility of microbial sulfide-induced copper-corrosion under field conditions. These results may fill knowledge gaps about the in situ microbial functions of marine biofilms and their effects on toxic-metal corrosion.https://www.frontiersin.org/article/10.3389/fmicb.2019.02863/fullmarinebiofilmbiocorrosioncopper-resistancemetal alloygene |
| spellingShingle | Yimeng Zhang Yimeng Zhang Yimeng Zhang Yimeng Zhang Yan Ma Yan Ma Yan Ma Ruiyong Zhang Binbin Zhang Binbin Zhang Binbin Zhang Xiaofan Zhai Xiaofan Zhai Xiaofan Zhai Wangqiang Li Wangqiang Li Wangqiang Li Liting Xu Liting Xu Liting Xu Quantong Jiang Quantong Jiang Quantong Jiang Jizhou Duan Jizhou Duan Jizhou Duan Baorong Hou Baorong Hou Baorong Hou Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms Frontiers in Microbiology marine biofilm biocorrosion copper-resistance metal alloy gene |
| title | Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms |
| title_full | Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms |
| title_fullStr | Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms |
| title_full_unstemmed | Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms |
| title_short | Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms |
| title_sort | metagenomic resolution of functional diversity in copper surface associated marine biofilms |
| topic | marine biofilm biocorrosion copper-resistance metal alloy gene |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02863/full |
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