<i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress
Recombinases are in part responsible for homologous recombination and genome integrity during DNA repair. <i>DMC1</i> has a typical RecA domain, and belongs to the recombinase superfamily. The reactive oxygen species (ROS) as a potent DNA damage agent is produced during seed germination...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/595 |
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| author | Xulin Wang Zhengqing Xie Zhaoran Tian Shuaipeng Wang Gongyao Shi Weiwei Chen Gangqiang Cao Baoming Tian Xiaochun Wei Luyue Zhang Fang Wei |
| author_facet | Xulin Wang Zhengqing Xie Zhaoran Tian Shuaipeng Wang Gongyao Shi Weiwei Chen Gangqiang Cao Baoming Tian Xiaochun Wei Luyue Zhang Fang Wei |
| author_sort | Xulin Wang |
| collection | DOAJ |
| description | Recombinases are in part responsible for homologous recombination and genome integrity during DNA repair. <i>DMC1</i> has a typical RecA domain, and belongs to the recombinase superfamily. The reactive oxygen species (ROS) as a potent DNA damage agent is produced during seed germination under stress conditions. DNA repair should be initiated immediately to allow for subsequent seedling development. In this study, we attempted to characterize the underlying mechanism of <i>BrDMC1</i> responsiveness to salinity stress using the RNA interference approach in <i>Brassica rapa</i> (<i>B. rapa</i>). Bioinformatics and expression pattern analysis revealed that <i>BrDMC1</i> only retained <i>BrDMC1</i>.A01 after the whole genome triplication (WGT) event and was primarily transcribed in flowers and seeds. <i>BrDMC1</i> had high activity in the promoter region during germination, according to histochemical GUS staining. The data showed that salt treatment reduced the germination rate, weakened seed vigor and decreased antioxidant enzyme activity, but increased oxidative damage in <i>BrDMC1</i>-RNAi seeds. Furthermore, the expression of stress-responsive genes and damage repair genes was significantly different in transgenic lines exposed to salt stress. Therefore, <i>BrDMC1</i> may respond to salt stress by controlling seed germination and the expression of stress-related and damage repair genes in <i>B. rapa</i>. |
| first_indexed | 2024-03-11T09:17:18Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
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| last_indexed | 2024-03-11T09:17:18Z |
| publishDate | 2023-02-01 |
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| series | Agronomy |
| spelling | doaj.art-bf416af20d7b4aed84a9200345e3c98c2023-11-16T18:36:54ZengMDPI AGAgronomy2073-43952023-02-0113259510.3390/agronomy13020595<i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt StressXulin Wang0Zhengqing Xie1Zhaoran Tian2Shuaipeng Wang3Gongyao Shi4Weiwei Chen5Gangqiang Cao6Baoming Tian7Xiaochun Wei8Luyue Zhang9Fang Wei10Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T and R Base of Zhengzhou University, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaRecombinases are in part responsible for homologous recombination and genome integrity during DNA repair. <i>DMC1</i> has a typical RecA domain, and belongs to the recombinase superfamily. The reactive oxygen species (ROS) as a potent DNA damage agent is produced during seed germination under stress conditions. DNA repair should be initiated immediately to allow for subsequent seedling development. In this study, we attempted to characterize the underlying mechanism of <i>BrDMC1</i> responsiveness to salinity stress using the RNA interference approach in <i>Brassica rapa</i> (<i>B. rapa</i>). Bioinformatics and expression pattern analysis revealed that <i>BrDMC1</i> only retained <i>BrDMC1</i>.A01 after the whole genome triplication (WGT) event and was primarily transcribed in flowers and seeds. <i>BrDMC1</i> had high activity in the promoter region during germination, according to histochemical GUS staining. The data showed that salt treatment reduced the germination rate, weakened seed vigor and decreased antioxidant enzyme activity, but increased oxidative damage in <i>BrDMC1</i>-RNAi seeds. Furthermore, the expression of stress-responsive genes and damage repair genes was significantly different in transgenic lines exposed to salt stress. Therefore, <i>BrDMC1</i> may respond to salt stress by controlling seed germination and the expression of stress-related and damage repair genes in <i>B. rapa</i>.https://www.mdpi.com/2073-4395/13/2/595<i>Brassica rapa</i><i>BrDMC1</i>RecAsalt stressreactive oxygen speciesseed germination |
| spellingShingle | Xulin Wang Zhengqing Xie Zhaoran Tian Shuaipeng Wang Gongyao Shi Weiwei Chen Gangqiang Cao Baoming Tian Xiaochun Wei Luyue Zhang Fang Wei <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress Agronomy <i>Brassica rapa</i> <i>BrDMC1</i> RecA salt stress reactive oxygen species seed germination |
| title | <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress |
| title_full | <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress |
| title_fullStr | <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress |
| title_full_unstemmed | <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress |
| title_short | <i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress |
| title_sort | i brdmc1 i a recombinase gene is involved in seed germination in i brassica rapa i under salt stress |
| topic | <i>Brassica rapa</i> <i>BrDMC1</i> RecA salt stress reactive oxygen species seed germination |
| url | https://www.mdpi.com/2073-4395/13/2/595 |
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