The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i>
Exercise induces the release of small extracellular vesicles (sEVs) into circulation that are postulated to mediate tissue cross-talk during exercise. We previously reported that pregnant individuals released greater levels of sEVs into circulation after exercise compared to matched non-pregnant con...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/11/3/857 |
| _version_ | 1797613367460888576 |
|---|---|
| author | Shuhiba Mohammad Jayonta Bhattacharjee Velislava Tzaneva Kelly Ann Hutchinson Madeeha Shaikh Danilo Fernandes da Silva Dylan Burger Kristi B. Adamo |
| author_facet | Shuhiba Mohammad Jayonta Bhattacharjee Velislava Tzaneva Kelly Ann Hutchinson Madeeha Shaikh Danilo Fernandes da Silva Dylan Burger Kristi B. Adamo |
| author_sort | Shuhiba Mohammad |
| collection | DOAJ |
| description | Exercise induces the release of small extracellular vesicles (sEVs) into circulation that are postulated to mediate tissue cross-talk during exercise. We previously reported that pregnant individuals released greater levels of sEVs into circulation after exercise compared to matched non-pregnant controls, but their biological functions remain unknown. In this study, sEVs isolated from the plasma of healthy pregnant and non-pregnant participants after a single bout of moderate-intensity exercise were evaluated for their impact on trophoblasts <i>in vitro</i>. Exercise-associated sEVs were found localized within the cytoplasm of BeWo choriocarcinoma cells, used to model trophoblasts in vitro. Exposure to exercise-associated sEVs did not significantly alter BeWo cell proliferation, gene expression of angiogenic growth factors <i>VEGF</i> and <i>PLGF</i>, or the release of the hormone human chorionic gonadotropin. The results from this pilot study support that exercise-associated sEVs could interact with trophoblasts in vitro, and warrant further investigation to reveal their potential role in communicating the effects of exercise to the maternal–fetal interface. |
| first_indexed | 2024-03-11T06:53:51Z |
| format | Article |
| id | doaj.art-bf4450b18af8403998504a2599559566 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T06:53:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-bf4450b18af8403998504a25995595662023-11-17T09:46:38ZengMDPI AGBiomedicines2227-90592023-03-0111385710.3390/biomedicines11030857The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i>Shuhiba Mohammad0Jayonta Bhattacharjee1Velislava Tzaneva2Kelly Ann Hutchinson3Madeeha Shaikh4Danilo Fernandes da Silva5Dylan Burger6Kristi B. Adamo7School of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaKidney Research Centre, Department of Cellular and Molecular Medicine, The Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON K1H 8L6, CanadaSchool of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, CanadaExercise induces the release of small extracellular vesicles (sEVs) into circulation that are postulated to mediate tissue cross-talk during exercise. We previously reported that pregnant individuals released greater levels of sEVs into circulation after exercise compared to matched non-pregnant controls, but their biological functions remain unknown. In this study, sEVs isolated from the plasma of healthy pregnant and non-pregnant participants after a single bout of moderate-intensity exercise were evaluated for their impact on trophoblasts <i>in vitro</i>. Exercise-associated sEVs were found localized within the cytoplasm of BeWo choriocarcinoma cells, used to model trophoblasts in vitro. Exposure to exercise-associated sEVs did not significantly alter BeWo cell proliferation, gene expression of angiogenic growth factors <i>VEGF</i> and <i>PLGF</i>, or the release of the hormone human chorionic gonadotropin. The results from this pilot study support that exercise-associated sEVs could interact with trophoblasts in vitro, and warrant further investigation to reveal their potential role in communicating the effects of exercise to the maternal–fetal interface.https://www.mdpi.com/2227-9059/11/3/857exercisephysical activitysmall extracellular vesiclesplacentatrophoblastpregnancy |
| spellingShingle | Shuhiba Mohammad Jayonta Bhattacharjee Velislava Tzaneva Kelly Ann Hutchinson Madeeha Shaikh Danilo Fernandes da Silva Dylan Burger Kristi B. Adamo The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> Biomedicines exercise physical activity small extracellular vesicles placenta trophoblast pregnancy |
| title | The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> |
| title_full | The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> |
| title_fullStr | The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> |
| title_full_unstemmed | The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> |
| title_short | The Influence of Exercise-Associated Small Extracellular Vesicles on Trophoblasts <i>In Vitro</i> |
| title_sort | influence of exercise associated small extracellular vesicles on trophoblasts i in vitro i |
| topic | exercise physical activity small extracellular vesicles placenta trophoblast pregnancy |
| url | https://www.mdpi.com/2227-9059/11/3/857 |
| work_keys_str_mv | AT shuhibamohammad theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT jayontabhattacharjee theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT velislavatzaneva theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT kellyannhutchinson theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT madeehashaikh theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT danilofernandesdasilva theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT dylanburger theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT kristibadamo theinfluenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT shuhibamohammad influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT jayontabhattacharjee influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT velislavatzaneva influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT kellyannhutchinson influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT madeehashaikh influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT danilofernandesdasilva influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT dylanburger influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi AT kristibadamo influenceofexerciseassociatedsmallextracellularvesiclesontrophoblastsiinvitroi |