The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes
Research into understanding the structure, composition and vertical transmission of crop seed microbiomes has intensified, although there is much less research into the seed microbiomes of crop wild relatives. Our previous study showed that the standard seed storage procedures (e.g., seed drying and...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/750 |
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| author | Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Desmond Auer Jacqueline Edwards German Spangenberg Timothy Sawbridge |
| author_facet | Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Desmond Auer Jacqueline Edwards German Spangenberg Timothy Sawbridge |
| author_sort | Ankush Chandel |
| collection | DOAJ |
| description | Research into understanding the structure, composition and vertical transmission of crop seed microbiomes has intensified, although there is much less research into the seed microbiomes of crop wild relatives. Our previous study showed that the standard seed storage procedures (e.g., seed drying and storage temperature) can influence the seed microbiome of domesticated <i>Glycine max</i>. In this study, we characterized the seed microbiota of <i>Glycine clandestina</i>, a perennial wild relative of soybean (<i>G. max</i> (L.) Merr.) to expand our understanding about the effect of other storage procedures such as the periodic regeneration of seed stocks to bulk up seed numbers and secure viability on the seed microbiome of said seed. The <i>G. clandestina</i> microbiota was analysed from Generation 1 (G1) and Generation 2 (G2) seed and from mature plant organs grown in two different soil treatments T (treatment [native soil + potting mix]) and C (control [potting mix only]). Our dataset showed that soil microbiota had a strong influence on next generation seed microbiota, with an increased contribution of root microbiota by 90% and seed transmissibility by 36.3% in G2 (T) seed. Interestingly, the G2 seed microbiota primarily consisted of an initially low abundance of taxa present in G1 seed. Overall, our results indicate that seed regeneration can affect the seed microbiome composition and using native soil from the location of the source plant can enhance the conservation of the native seed microbiota. |
| first_indexed | 2024-03-09T13:16:48Z |
| format | Article |
| id | doaj.art-bf4a3fc030e740688b40f67423dddc78 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:48Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bf4a3fc030e740688b40f67423dddc782023-11-30T21:35:13ZengMDPI AGMicroorganisms2076-26072022-03-0110475010.3390/microorganisms10040750The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial MicrobiomesAnkush Chandel0Ross Mann1Jatinder Kaur2Sally Norton3Desmond Auer4Jacqueline Edwards5German Spangenberg6Timothy Sawbridge7Agriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, Australian Grains Genebank, Horsham, VIC 3400, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaResearch into understanding the structure, composition and vertical transmission of crop seed microbiomes has intensified, although there is much less research into the seed microbiomes of crop wild relatives. Our previous study showed that the standard seed storage procedures (e.g., seed drying and storage temperature) can influence the seed microbiome of domesticated <i>Glycine max</i>. In this study, we characterized the seed microbiota of <i>Glycine clandestina</i>, a perennial wild relative of soybean (<i>G. max</i> (L.) Merr.) to expand our understanding about the effect of other storage procedures such as the periodic regeneration of seed stocks to bulk up seed numbers and secure viability on the seed microbiome of said seed. The <i>G. clandestina</i> microbiota was analysed from Generation 1 (G1) and Generation 2 (G2) seed and from mature plant organs grown in two different soil treatments T (treatment [native soil + potting mix]) and C (control [potting mix only]). Our dataset showed that soil microbiota had a strong influence on next generation seed microbiota, with an increased contribution of root microbiota by 90% and seed transmissibility by 36.3% in G2 (T) seed. Interestingly, the G2 seed microbiota primarily consisted of an initially low abundance of taxa present in G1 seed. Overall, our results indicate that seed regeneration can affect the seed microbiome composition and using native soil from the location of the source plant can enhance the conservation of the native seed microbiota.https://www.mdpi.com/2076-2607/10/4/75016S rRNAseedbankseed vault<i>Glycine clandestina</i>seed microbiomebacterial diversity |
| spellingShingle | Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Desmond Auer Jacqueline Edwards German Spangenberg Timothy Sawbridge The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes Microorganisms 16S rRNA seedbank seed vault <i>Glycine clandestina</i> seed microbiome bacterial diversity |
| title | The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes |
| title_full | The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes |
| title_fullStr | The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes |
| title_full_unstemmed | The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes |
| title_short | The Role of Soil Microbial Diversity in the Conservation of Native Seed Bacterial Microbiomes |
| title_sort | role of soil microbial diversity in the conservation of native seed bacterial microbiomes |
| topic | 16S rRNA seedbank seed vault <i>Glycine clandestina</i> seed microbiome bacterial diversity |
| url | https://www.mdpi.com/2076-2607/10/4/750 |
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