Improvement of the Catalytic Ability of a Thermostable and Acidophilic β-Mannanase Using a Consensus Sequence Design Strategy

Improvement of the Catalytic Ability of a Thermostable and Acidophilic β-Mannanase Using a Consensus Sequence Design Strategy

In order to improve the catalytic efficiency of a thermostable and acidophilic β-mannanase (ManAK; derived from marine Aspergillus kawachii IFO 4308), three mutants were designed by amino acid sequence consensus analysis with a second β-mannanase (ManCbs), which also belongs to the glycoside hydrola...

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Bibliographic Details
Main Authors: Qingping Liang, Yuming Zhan, Mingxue Yuan, Linyuan Cao, Changliang Zhu, Haijin Mou, Zhemin Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Microbiology
Subjects:
β-mannanase
consensus sequence design
catalytic ability
thermostability
rational design
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.722347/full

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