Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System
In this study, we successfully established a co-culture system of bovine preadipocytes and myoblasts to explore the effect of exogenous addition of Neudesin neurotrophic factor (NENF) recombinant protein on the differentiation of adipocytes and myoblasts in co-culture. The optimal concentration of N...
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MDPI AG
2020-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/1/34 |
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| author | Anqi Li Xiaotong Su Yaning Wang Gong Cheng Linsen Zan Hongbao Wang |
| author_facet | Anqi Li Xiaotong Su Yaning Wang Gong Cheng Linsen Zan Hongbao Wang |
| author_sort | Anqi Li |
| collection | DOAJ |
| description | In this study, we successfully established a co-culture system of bovine preadipocytes and myoblasts to explore the effect of exogenous addition of Neudesin neurotrophic factor (NENF) recombinant protein on the differentiation of adipocytes and myoblasts in co-culture. The optimal concentration of NENF recombinant protein was 100 pg/mL. <i>NENF</i> promoted the differentiation of bovine preadipocytes and inhibited the differentiation of bovine myoblasts when the cells were cultured separately. After adding NENF recombinant protein to the co-culture system, the accumulation of lipid droplets in bovine preadipocytes decreased, but the differentiation of bovine myoblasts did not change significantly. The results of real-time quantitative PCR (RT-qPCR) and Western blot showed that the expression levels of adipogenesis-related factors such as <i>PPARγ</i>, <i>FABP4</i> and <i>FASN</i> were significantly down-regulated at the mRNA and protein levels in adipocytes, while myogenic marker genes such as <i>MYOD1</i>, <i>MYOG</i> and <i>MYHC</i> had no significant changes at the mRNA or protein levels in myoblasts. This differs from, and potentially conflicts with, the monoculture system, where <i>NENF</i> expression in each cell type changed with the cell microenvironment. Consequently, the molecular mechanism of marbling beef formation cannot be fully revealed using monocultures of adipocytes or myocytes. |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:44:51Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-bf4bd70fbc4c4786a70b3450cfb9d0812023-11-21T02:41:26ZengMDPI AGAnimals2076-26152020-12-011113410.3390/ani11010034Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture SystemAnqi Li0Xiaotong Su1Yaning Wang2Gong Cheng3Linsen Zan4Hongbao Wang5College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaIn this study, we successfully established a co-culture system of bovine preadipocytes and myoblasts to explore the effect of exogenous addition of Neudesin neurotrophic factor (NENF) recombinant protein on the differentiation of adipocytes and myoblasts in co-culture. The optimal concentration of NENF recombinant protein was 100 pg/mL. <i>NENF</i> promoted the differentiation of bovine preadipocytes and inhibited the differentiation of bovine myoblasts when the cells were cultured separately. After adding NENF recombinant protein to the co-culture system, the accumulation of lipid droplets in bovine preadipocytes decreased, but the differentiation of bovine myoblasts did not change significantly. The results of real-time quantitative PCR (RT-qPCR) and Western blot showed that the expression levels of adipogenesis-related factors such as <i>PPARγ</i>, <i>FABP4</i> and <i>FASN</i> were significantly down-regulated at the mRNA and protein levels in adipocytes, while myogenic marker genes such as <i>MYOD1</i>, <i>MYOG</i> and <i>MYHC</i> had no significant changes at the mRNA or protein levels in myoblasts. This differs from, and potentially conflicts with, the monoculture system, where <i>NENF</i> expression in each cell type changed with the cell microenvironment. Consequently, the molecular mechanism of marbling beef formation cannot be fully revealed using monocultures of adipocytes or myocytes.https://www.mdpi.com/2076-2615/11/1/34bovineNENFpreadipocytesmyoblastsco-culture |
| spellingShingle | Anqi Li Xiaotong Su Yaning Wang Gong Cheng Linsen Zan Hongbao Wang Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System Animals bovine NENF preadipocytes myoblasts co-culture |
| title | Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System |
| title_full | Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System |
| title_fullStr | Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System |
| title_full_unstemmed | Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System |
| title_short | Effect of Neudesin Neurotrophic Factor on Differentiation of Bovine Preadipocytes and Myoblasts in a Co-Culture System |
| title_sort | effect of neudesin neurotrophic factor on differentiation of bovine preadipocytes and myoblasts in a co culture system |
| topic | bovine NENF preadipocytes myoblasts co-culture |
| url | https://www.mdpi.com/2076-2615/11/1/34 |
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