Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampere...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1994-09-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032 |
| _version_ | 1797721380606705664 |
|---|---|
| author | Win Degrave Octavio Fernandes David Campbell Marcelo Bozza Ulisses Lopes |
| author_facet | Win Degrave Octavio Fernandes David Campbell Marcelo Bozza Ulisses Lopes |
| author_sort | Win Degrave |
| collection | DOAJ |
| description | The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported. |
| first_indexed | 2024-03-12T09:32:32Z |
| format | Article |
| id | doaj.art-bf4f4d8d04874924814ad0164d705151 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:32:32Z |
| publishDate | 1994-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-bf4f4d8d04874924814ad0164d7051512023-09-02T13:53:49ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601994-09-0189346346910.1590/S0074-02761994000300032Use of molecular probes and PCR for detection and typing of Leishmania - a mini-reviewWin DegraveOctavio FernandesDavid CampbellMarcelo BozzaUlisses LopesThe use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032Leishmaniapolymerase chain reactionhybridizationmolecular probesmini-exon geneminicirclekDNA |
| spellingShingle | Win Degrave Octavio Fernandes David Campbell Marcelo Bozza Ulisses Lopes Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review Memorias do Instituto Oswaldo Cruz Leishmania polymerase chain reaction hybridization molecular probes mini-exon gene minicircle kDNA |
| title | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review |
| title_full | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review |
| title_fullStr | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review |
| title_full_unstemmed | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review |
| title_short | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review |
| title_sort | use of molecular probes and pcr for detection and typing of leishmania a mini review |
| topic | Leishmania polymerase chain reaction hybridization molecular probes mini-exon gene minicircle kDNA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032 |
| work_keys_str_mv | AT windegrave useofmolecularprobesandpcrfordetectionandtypingofleishmaniaaminireview AT octaviofernandes useofmolecularprobesandpcrfordetectionandtypingofleishmaniaaminireview AT davidcampbell useofmolecularprobesandpcrfordetectionandtypingofleishmaniaaminireview AT marcelobozza useofmolecularprobesandpcrfordetectionandtypingofleishmaniaaminireview AT ulisseslopes useofmolecularprobesandpcrfordetectionandtypingofleishmaniaaminireview |