Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering
Herein, I review our recent work toward developing methods for generating three-dimensional (3D) droplet arrays driven by capillarity. Microdroplet array-based systems are useful for bioassays and bioengineering because they require only small amounts of samples and reagents and provide the high thr...
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| Format: | Article |
| Language: | English |
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The Biophysical Society of Japan
2023-06-01
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| Series: | Biophysics and Physicobiology |
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| Online Access: | https://doi.org/10.2142/biophysico.bppb-v20.0029 |
| _version_ | 1827918441348071424 |
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| author | Hiroki Yasuga |
| author_facet | Hiroki Yasuga |
| author_sort | Hiroki Yasuga |
| collection | DOAJ |
| description | Herein, I review our recent work toward developing methods for generating three-dimensional (3D) droplet arrays driven by capillarity. Microdroplet array-based systems are useful for bioassays and bioengineering because they require only small amounts of samples and reagents and provide the high throughput. Various methods have been developed for preparing droplet arrays, among which methods based on capillarity have attracted considerable attention owing to their simplicity. I and collaborators have developed such methods based on capillary flow, including a method for preparing droplet arrays via oil–water replacement. We recently proposed our own concept of “fluid–fluid interfacial energy driven 3D structure emergence in a micropillar scaffold (FLUID3EAMS)” and its application. FLUID3EAMS allows a 3D droplet (or hydrogel bead) array to be generated in a micropillar scaffold by passing a fluid–fluid interface through the scaffold. This approach is useful for applications requiring ordered or arrayed microdroplets in biosensors, biophysics, biology, and tissue engineering. This review is an extended version of the article “FLUID3EAMS: Fluid–Fluid Interfacial Energy Driven 3D Structure Emergence in a Micropillar Scaffold and Development in Bioengineering” published in Seibutsu Butsuri (vol. 62, p. 110–113, 2022). |
| first_indexed | 2024-03-13T03:42:53Z |
| format | Article |
| id | doaj.art-bf541846b6f343a39d7ff8d7ededa974 |
| institution | Directory Open Access Journal |
| issn | 2189-4779 |
| language | English |
| last_indexed | 2024-03-13T03:42:53Z |
| publishDate | 2023-06-01 |
| publisher | The Biophysical Society of Japan |
| record_format | Article |
| series | Biophysics and Physicobiology |
| spelling | doaj.art-bf541846b6f343a39d7ff8d7ededa9742023-06-23T05:18:43ZengThe Biophysical Society of JapanBiophysics and Physicobiology2189-47792023-06-012010.2142/biophysico.bppb-v20.0029Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineeringHiroki Yasuga0Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8564, JapanHerein, I review our recent work toward developing methods for generating three-dimensional (3D) droplet arrays driven by capillarity. Microdroplet array-based systems are useful for bioassays and bioengineering because they require only small amounts of samples and reagents and provide the high throughput. Various methods have been developed for preparing droplet arrays, among which methods based on capillarity have attracted considerable attention owing to their simplicity. I and collaborators have developed such methods based on capillary flow, including a method for preparing droplet arrays via oil–water replacement. We recently proposed our own concept of “fluid–fluid interfacial energy driven 3D structure emergence in a micropillar scaffold (FLUID3EAMS)” and its application. FLUID3EAMS allows a 3D droplet (or hydrogel bead) array to be generated in a micropillar scaffold by passing a fluid–fluid interface through the scaffold. This approach is useful for applications requiring ordered or arrayed microdroplets in biosensors, biophysics, biology, and tissue engineering. This review is an extended version of the article “FLUID3EAMS: Fluid–Fluid Interfacial Energy Driven 3D Structure Emergence in a Micropillar Scaffold and Development in Bioengineering” published in Seibutsu Butsuri (vol. 62, p. 110–113, 2022).https://doi.org/10.2142/biophysico.bppb-v20.0029microfluidicsinterfacesmicrostructurecapillary flowfluid3eams |
| spellingShingle | Hiroki Yasuga Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering Biophysics and Physicobiology microfluidics interfaces microstructure capillary flow fluid3eams |
| title | Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| title_full | Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| title_fullStr | Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| title_full_unstemmed | Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| title_short | Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| title_sort | methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering |
| topic | microfluidics interfaces microstructure capillary flow fluid3eams |
| url | https://doi.org/10.2142/biophysico.bppb-v20.0029 |
| work_keys_str_mv | AT hirokiyasuga methodstospontaneouslygeneratethreedimensionallyarrayedmicrodropletstriggeredbycapillarityforbioassaysandbioengineering |