Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion
Various infarct sizes induced by middle cerebral artery occlusion (MCAO) generate inconsistent outcomes for stroke preclinical study. Monitoring cerebral hemodynamics may help to verify the outcome of MCAO. The aim of this study was to investigate the changes in brain tissue optical properties by fr...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10318 |
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| author | Chun-Wei Wu Jia-Jin Chen Chou-Ching K. Lin Chien-An Chen Chun-Ie Wu Ing-Shiou Hwang Tsung-Hsun Hsieh Bor-Shing Lin Chih-Wei Peng |
| author_facet | Chun-Wei Wu Jia-Jin Chen Chou-Ching K. Lin Chien-An Chen Chun-Ie Wu Ing-Shiou Hwang Tsung-Hsun Hsieh Bor-Shing Lin Chih-Wei Peng |
| author_sort | Chun-Wei Wu |
| collection | DOAJ |
| description | Various infarct sizes induced by middle cerebral artery occlusion (MCAO) generate inconsistent outcomes for stroke preclinical study. Monitoring cerebral hemodynamics may help to verify the outcome of MCAO. The aim of this study was to investigate the changes in brain tissue optical properties by frequency-domain near-infrared spectroscopy (FD-NIRS), and establish the relationship between cerebral hemodynamics and infarct variation in MCAO model. The rats were undergone transient MCAO using intraluminal filament. The optical properties and hemodynamics were measured by placing the FD-NIRS probes on the scalp of the head before, during, and at various time-courses after MCAO. Bimodal infarction severities were observed after the same 90-min MCAO condition. Significant decreases in concentrations of oxygenated hemoglobin ([HbO]) and total hemoglobin ([HbT]), tissue oxygenation saturation (StO<sub>2</sub>), absorption coefficient (μa) at 830 nm, and reduced scattering coefficient (μs’) at both 690 and 830 nm were detected during the occlusion in the severe infarction but not the mild one. Of note, the significant increases in [HbO], [HbT], StO<sub>2</sub>, and μa at both 690 and 830 nm were found on day 3; and increases in μs’ at both 690 and 830 nm were found on day 2 and day 3 after MCAO, respectively. The interhemispheric correlation coefficient (IHCC) was computed from low-frequency hemodynamic oscillation of both hemispheres. Lower IHCCs standing for interhemispheric desynchronizations were found in both mild and severe infarction during occlusion, and only in severe infarction after reperfusion. Our finding supports that sequential FD-NIRS parameters may associated with the severity of the infarction in MCAO model, and the consequent pathologies such as vascular dysfunction and brain edema. Further study is required to validate the potential use of FD-NIRS as a monitor for MCAO verification. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:49:02Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-bf54b89dd1224763b3ef8164c2fb58d72023-11-23T16:39:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181031810.3390/ijms231810318Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery OcclusionChun-Wei Wu0Jia-Jin Chen1Chou-Ching K. Lin2Chien-An Chen3Chun-Ie Wu4Ing-Shiou Hwang5Tsung-Hsun Hsieh6Bor-Shing Lin7Chih-Wei Peng8School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, TaiwanDepartment of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Neurology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan 70101, TaiwanInstitute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Physical Therapy and Graduate Institute of Rehabilitation Science, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Computer Science and Information Engineering, National Taipei University, New Taipei City 237303, TaiwanSchool of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, TaiwanVarious infarct sizes induced by middle cerebral artery occlusion (MCAO) generate inconsistent outcomes for stroke preclinical study. Monitoring cerebral hemodynamics may help to verify the outcome of MCAO. The aim of this study was to investigate the changes in brain tissue optical properties by frequency-domain near-infrared spectroscopy (FD-NIRS), and establish the relationship between cerebral hemodynamics and infarct variation in MCAO model. The rats were undergone transient MCAO using intraluminal filament. The optical properties and hemodynamics were measured by placing the FD-NIRS probes on the scalp of the head before, during, and at various time-courses after MCAO. Bimodal infarction severities were observed after the same 90-min MCAO condition. Significant decreases in concentrations of oxygenated hemoglobin ([HbO]) and total hemoglobin ([HbT]), tissue oxygenation saturation (StO<sub>2</sub>), absorption coefficient (μa) at 830 nm, and reduced scattering coefficient (μs’) at both 690 and 830 nm were detected during the occlusion in the severe infarction but not the mild one. Of note, the significant increases in [HbO], [HbT], StO<sub>2</sub>, and μa at both 690 and 830 nm were found on day 3; and increases in μs’ at both 690 and 830 nm were found on day 2 and day 3 after MCAO, respectively. The interhemispheric correlation coefficient (IHCC) was computed from low-frequency hemodynamic oscillation of both hemispheres. Lower IHCCs standing for interhemispheric desynchronizations were found in both mild and severe infarction during occlusion, and only in severe infarction after reperfusion. Our finding supports that sequential FD-NIRS parameters may associated with the severity of the infarction in MCAO model, and the consequent pathologies such as vascular dysfunction and brain edema. Further study is required to validate the potential use of FD-NIRS as a monitor for MCAO verification.https://www.mdpi.com/1422-0067/23/18/10318ischemic strokemiddle cerebral artery occlusionnear infrared spectroscopycerebral hemodynamicsinterhemispheric correlation coefficientcerebral blood flow |
| spellingShingle | Chun-Wei Wu Jia-Jin Chen Chou-Ching K. Lin Chien-An Chen Chun-Ie Wu Ing-Shiou Hwang Tsung-Hsun Hsieh Bor-Shing Lin Chih-Wei Peng Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion International Journal of Molecular Sciences ischemic stroke middle cerebral artery occlusion near infrared spectroscopy cerebral hemodynamics interhemispheric correlation coefficient cerebral blood flow |
| title | Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion |
| title_full | Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion |
| title_fullStr | Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion |
| title_full_unstemmed | Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion |
| title_short | Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion |
| title_sort | hemodynamics and tissue optical properties in bimodal infarctions induced by middle cerebral artery occlusion |
| topic | ischemic stroke middle cerebral artery occlusion near infrared spectroscopy cerebral hemodynamics interhemispheric correlation coefficient cerebral blood flow |
| url | https://www.mdpi.com/1422-0067/23/18/10318 |
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