Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition
The main goal of this study was to use hybrid delivery system for effective transportation of temoporfin (meta-tetrakis(3-hydroxyphenyl)chlorin, mTHPC) to target tissue. We suggested to couple two independent delivery systems (liposomes and inclusion complexes) to achieve drug-in-cyclodextrin-in-lip...
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| Format: | Article |
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MDPI AG
2018-10-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/10/847 |
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| author | Ilya Yakavets Henri-Pierre Lassalle Dietrich Scheglmann Arno Wiehe Vladimir Zorin Lina Bezdetnaya |
| author_facet | Ilya Yakavets Henri-Pierre Lassalle Dietrich Scheglmann Arno Wiehe Vladimir Zorin Lina Bezdetnaya |
| author_sort | Ilya Yakavets |
| collection | DOAJ |
| description | The main goal of this study was to use hybrid delivery system for effective transportation of temoporfin (meta-tetrakis(3-hydroxyphenyl)chlorin, mTHPC) to target tissue. We suggested to couple two independent delivery systems (liposomes and inclusion complexes) to achieve drug-in-cyclodextrin-in-liposome (DCL) nanoconstructs. We further optimized the composition of DCLs, aiming to alter in a more favorable way a distribution of temoporfin in tumor tissue. We have prepared DCLs with different compositions varying the concentration of mTHPC and the type of β-cyclodextrin (β-CD) derivatives (Hydroxypropyl-, Methyl- and Trimethyl-β-CD). DCLs were prepared by thin-hydration technique and mTHPC/β-CD complexes were added at hydration step. The size was about 135 nm with the surface charge of (−38 mV). We have demonstrated that DCLs are stable and almost all mTHPC is bound to β-CDs in the inner aqueous liposome core. Among all tested DCLs, trimethyl-β-CD-based DCL demonstrated a homogenous accumulation of mTHPC across tumor spheroid volume, thus supposing optimal mTHPC distribution. |
| first_indexed | 2024-12-20T16:45:15Z |
| format | Article |
| id | doaj.art-bf555d3539d44df39d68c23c8d279ae1 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T16:45:15Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-bf555d3539d44df39d68c23c8d279ae12022-12-21T19:32:57ZengMDPI AGNanomaterials2079-49912018-10-0181084710.3390/nano8100847nano8100847Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of CompositionIlya Yakavets0Henri-Pierre Lassalle1Dietrich Scheglmann2Arno Wiehe3Vladimir Zorin4Lina Bezdetnaya5Centre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique UMR 7039, Université de Lorraine, Campus Sciences, Boulevard des Aiguillette, 54506 Vandoeuvre-lès-Nancy, FranceCentre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique UMR 7039, Université de Lorraine, Campus Sciences, Boulevard des Aiguillette, 54506 Vandoeuvre-lès-Nancy, FranceBiolitec Research GmbH, Otto-Schott-Strasse 15, 07745 Jena, GermanyBiolitec Research GmbH, Otto-Schott-Strasse 15, 07745 Jena, GermanyLaboratory of Biophysics and Biotechnology, Belarusian State University, 4 Nezavisimosti Avenue, 220030 Minsk, BelarusCentre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique UMR 7039, Université de Lorraine, Campus Sciences, Boulevard des Aiguillette, 54506 Vandoeuvre-lès-Nancy, FranceThe main goal of this study was to use hybrid delivery system for effective transportation of temoporfin (meta-tetrakis(3-hydroxyphenyl)chlorin, mTHPC) to target tissue. We suggested to couple two independent delivery systems (liposomes and inclusion complexes) to achieve drug-in-cyclodextrin-in-liposome (DCL) nanoconstructs. We further optimized the composition of DCLs, aiming to alter in a more favorable way a distribution of temoporfin in tumor tissue. We have prepared DCLs with different compositions varying the concentration of mTHPC and the type of β-cyclodextrin (β-CD) derivatives (Hydroxypropyl-, Methyl- and Trimethyl-β-CD). DCLs were prepared by thin-hydration technique and mTHPC/β-CD complexes were added at hydration step. The size was about 135 nm with the surface charge of (−38 mV). We have demonstrated that DCLs are stable and almost all mTHPC is bound to β-CDs in the inner aqueous liposome core. Among all tested DCLs, trimethyl-β-CD-based DCL demonstrated a homogenous accumulation of mTHPC across tumor spheroid volume, thus supposing optimal mTHPC distribution.http://www.mdpi.com/2079-4991/8/10/847temoporfindrug-in-cyclodextrin-in-liposomenanoparticlesmulticellular tumor spheroidsflow cytometryphotodynamic therapy |
| spellingShingle | Ilya Yakavets Henri-Pierre Lassalle Dietrich Scheglmann Arno Wiehe Vladimir Zorin Lina Bezdetnaya Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition Nanomaterials temoporfin drug-in-cyclodextrin-in-liposome nanoparticles multicellular tumor spheroids flow cytometry photodynamic therapy |
| title | Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition |
| title_full | Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition |
| title_fullStr | Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition |
| title_full_unstemmed | Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition |
| title_short | Temoporfin-in-Cyclodextrin-in-Liposome—A New Approach for Anticancer Drug Delivery: The Optimization of Composition |
| title_sort | temoporfin in cyclodextrin in liposome a new approach for anticancer drug delivery the optimization of composition |
| topic | temoporfin drug-in-cyclodextrin-in-liposome nanoparticles multicellular tumor spheroids flow cytometry photodynamic therapy |
| url | http://www.mdpi.com/2079-4991/8/10/847 |
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