Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion.
BACKGROUND: Intestinal ischemia/reperfusion (I/R) induces the desquamation of the intestinal epithelium, increases the intestinal permeability, and in patients often causes fatal conditions including sepsis and multiple organ failure. Keratinocyte growth factor (KGF) increases intestinal growth, alt...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3441439?pdf=render |
| _version_ | 1818362980269555712 |
|---|---|
| author | Yujiao Cai Wensheng Wang Hongying Liang Lihua Sun Daniel H Teitelbaum Hua Yang |
| author_facet | Yujiao Cai Wensheng Wang Hongying Liang Lihua Sun Daniel H Teitelbaum Hua Yang |
| author_sort | Yujiao Cai |
| collection | DOAJ |
| description | BACKGROUND: Intestinal ischemia/reperfusion (I/R) induces the desquamation of the intestinal epithelium, increases the intestinal permeability, and in patients often causes fatal conditions including sepsis and multiple organ failure. Keratinocyte growth factor (KGF) increases intestinal growth, although little is known about KGF activity on intestinal function after intestinal I/R. We hypothesized that KGF administration would improve the intestinal function in a mouse model of intestinal I/R. METHODS: Adult C57BL/6J mice were randomized to three groups: Sham, I/R group and I/R+KGF group. Mice were killed on day 5, and the small bowel was harvested for histology, wet weight, RNA and protein content analysis. Epithelial cell (EC) proliferation was detected by immunohistochemistry for PCNA, and apoptosis was determined by TUNEL staining. The expressions of Claudin-1 and ZO-1 were detected by immunohistochemistry. Epithelial barrier function was assessed with transepithelial resistance (TER). RESULTS: KGF significantly increased the intestinal wet weight, contents of intestinal protein and RNA, villus height, crypt depth and crypt cell proliferation, while KGF resulted in the decrease of epithelial apoptosis. KGF also stimulated the recovery of mucosal structures and attenuated the disrupted distribution of TJ proteins. Moreover, KGF attenuated the intestinal I/R-induced decrease in TER and maintained the intestinal barrier function. CONCLUSION: KGF administration improves the epithelial structure and barrier function in a mouse model of intestinal I/R. This suggests that KGF may have clinical applicability. |
| first_indexed | 2024-12-13T21:41:12Z |
| format | Article |
| id | doaj.art-bf55f1630a0d4db8b1b110e60fbdcac2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:41:12Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bf55f1630a0d4db8b1b110e60fbdcac22022-12-21T23:30:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4477210.1371/journal.pone.0044772Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion.Yujiao CaiWensheng WangHongying LiangLihua SunDaniel H TeitelbaumHua YangBACKGROUND: Intestinal ischemia/reperfusion (I/R) induces the desquamation of the intestinal epithelium, increases the intestinal permeability, and in patients often causes fatal conditions including sepsis and multiple organ failure. Keratinocyte growth factor (KGF) increases intestinal growth, although little is known about KGF activity on intestinal function after intestinal I/R. We hypothesized that KGF administration would improve the intestinal function in a mouse model of intestinal I/R. METHODS: Adult C57BL/6J mice were randomized to three groups: Sham, I/R group and I/R+KGF group. Mice were killed on day 5, and the small bowel was harvested for histology, wet weight, RNA and protein content analysis. Epithelial cell (EC) proliferation was detected by immunohistochemistry for PCNA, and apoptosis was determined by TUNEL staining. The expressions of Claudin-1 and ZO-1 were detected by immunohistochemistry. Epithelial barrier function was assessed with transepithelial resistance (TER). RESULTS: KGF significantly increased the intestinal wet weight, contents of intestinal protein and RNA, villus height, crypt depth and crypt cell proliferation, while KGF resulted in the decrease of epithelial apoptosis. KGF also stimulated the recovery of mucosal structures and attenuated the disrupted distribution of TJ proteins. Moreover, KGF attenuated the intestinal I/R-induced decrease in TER and maintained the intestinal barrier function. CONCLUSION: KGF administration improves the epithelial structure and barrier function in a mouse model of intestinal I/R. This suggests that KGF may have clinical applicability.http://europepmc.org/articles/PMC3441439?pdf=render |
| spellingShingle | Yujiao Cai Wensheng Wang Hongying Liang Lihua Sun Daniel H Teitelbaum Hua Yang Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. PLoS ONE |
| title | Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. |
| title_full | Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. |
| title_fullStr | Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. |
| title_full_unstemmed | Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. |
| title_short | Keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia/reperfusion. |
| title_sort | keratinocyte growth factor improves epithelial structure and function in a mouse model of intestinal ischemia reperfusion |
| url | http://europepmc.org/articles/PMC3441439?pdf=render |
| work_keys_str_mv | AT yujiaocai keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion AT wenshengwang keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion AT hongyingliang keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion AT lihuasun keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion AT danielhteitelbaum keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion AT huayang keratinocytegrowthfactorimprovesepithelialstructureandfunctioninamousemodelofintestinalischemiareperfusion |