Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester
Currently, one of the most actual health problems in the world is the problem of a spontaneous abortion. Chromosomal abnormalities are one of the main reasons of early abortion, stillbirth or the birth of a child with multiple congenital malformations. The results of karyotyping of chorionic villi f...
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| Format: | Article |
| Language: | Russian |
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V. N. Karazin Kharkiv National University
2021-12-01
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| Series: | Вісник Харківського Національного Університету Імені В.Н. Каразіна: Серія Біологія |
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| Online Access: | https://periodicals.karazin.ua/biology/article/view/18835 |
| _version_ | 1828056188262023168 |
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| author | O. Feskov I. Feskova Ye. Zhylkova V. Rudenko O. Blazhko |
| author_facet | O. Feskov I. Feskova Ye. Zhylkova V. Rudenko O. Blazhko |
| author_sort | O. Feskov |
| collection | DOAJ |
| description | Currently, one of the most actual health problems in the world is the problem of a spontaneous abortion. Chromosomal abnormalities are one of the main reasons of early abortion, stillbirth or the birth of a child with multiple congenital malformations. The results of karyotyping of chorionic villi from 149 patients with diagnoses of "undeveloped pregnancy" or "anembryonia" were presented in the article. Metaphase chromosomes were used for cytogenetic research of the embryos. The samples were obtained from cytotrophoblast cells of chorionic villi by the "direct" method, without culture, according to standard technique. A GTG-method was used for staining the chromosome slides. The cytogenetic study of chorionic villi makes it possible to identify the karyotype abnormalities, which caused the fading of pregnancy, since the chorion karyotype corresponds to the embryo karyotype The slide analysis was carried out in accordance with the international system of the human cytogenetic nomenclature. As a result of the cytogenetic study, changes in the karyotype were revealed in 53.39 % of the samples, namely, genomic mutations – aneuploidy and polyploidy. Among the aneuploidies, we found the embryos with trisomy in the autosomes 5, 13, 16, 18, 20, 21, and 22. In addition, the karyotypes with trisomy and monosomy of the X chromosome, as well as the male karyotypes with an additional copy of the X or Y chromosome were present The polyploids in the study group were represented by triploid and tetraploid karyotypes of embryos. All the above mentioned karyotype disorders were found in both full and mosaic forms. Further, some non-developing embryos contained a chimeric karyotype - "chi46,XX/46,XY". In the study group, the prevailing were the embryos with a tetraploid karyotype in a mosaic form (6.71 %), Klinefelter syndrome in a mosaic form (6.04 %), and a triploid karyotype in a mosaic form (6.04 %). A statistically significant increase in the incidence of stillbirths with mosaic tetraploid karyotype was found in 2020. The share of this pathology was 25. %. Currently, there is no reliable information on the effect of the SARS-CoV-2 virus on the embryonic genome. Nevertheless, it is known that members of the coronavirus family are responsible for serious complications during pregnancy - pregnancy fading, fetal growth retardation, premature birth, death of the mother or fetal death in the neonatal period. In this connection, we can hypothesize that mitotic disorders and, as a consequence, appearance of embryos with a tetraploid karyotype seem to be associated with a mild SARS-CoV-2 infection that occurs in pregnant women in an inconspicuous form. |
| first_indexed | 2024-04-10T20:51:40Z |
| format | Article |
| id | doaj.art-bf594c1141ab4b6caa07f3576011e3e4 |
| institution | Directory Open Access Journal |
| issn | 2075-5457 2220-9697 |
| language | Russian |
| last_indexed | 2024-04-10T20:51:40Z |
| publishDate | 2021-12-01 |
| publisher | V. N. Karazin Kharkiv National University |
| record_format | Article |
| series | Вісник Харківського Національного Університету Імені В.Н. Каразіна: Серія Біологія |
| spelling | doaj.art-bf594c1141ab4b6caa07f3576011e3e42023-01-23T11:01:34ZrusV. N. Karazin Kharkiv National UniversityВісник Харківського Національного Університету Імені В.Н. Каразіна: Серія Біологія2075-54572220-96972021-12-0137435010.26565/2075-5457-2021-37-318835Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimesterO. Feskov0I. Feskova1Ye. Zhylkova2V. Rudenko3O. Blazhko4Center of Human Reproduction «Clinic of Professor Feskov O.»Center of Human Reproduction «Clinic of Professor Feskov O.»Center of Human Reproduction «Clinic of Professor Feskov O.»Center of Human Reproduction «Clinic of Professor Feskov O.»Center of Human Reproduction «Clinic of Professor Feskov O.»Currently, one of the most actual health problems in the world is the problem of a spontaneous abortion. Chromosomal abnormalities are one of the main reasons of early abortion, stillbirth or the birth of a child with multiple congenital malformations. The results of karyotyping of chorionic villi from 149 patients with diagnoses of "undeveloped pregnancy" or "anembryonia" were presented in the article. Metaphase chromosomes were used for cytogenetic research of the embryos. The samples were obtained from cytotrophoblast cells of chorionic villi by the "direct" method, without culture, according to standard technique. A GTG-method was used for staining the chromosome slides. The cytogenetic study of chorionic villi makes it possible to identify the karyotype abnormalities, which caused the fading of pregnancy, since the chorion karyotype corresponds to the embryo karyotype The slide analysis was carried out in accordance with the international system of the human cytogenetic nomenclature. As a result of the cytogenetic study, changes in the karyotype were revealed in 53.39 % of the samples, namely, genomic mutations – aneuploidy and polyploidy. Among the aneuploidies, we found the embryos with trisomy in the autosomes 5, 13, 16, 18, 20, 21, and 22. In addition, the karyotypes with trisomy and monosomy of the X chromosome, as well as the male karyotypes with an additional copy of the X or Y chromosome were present The polyploids in the study group were represented by triploid and tetraploid karyotypes of embryos. All the above mentioned karyotype disorders were found in both full and mosaic forms. Further, some non-developing embryos contained a chimeric karyotype - "chi46,XX/46,XY". In the study group, the prevailing were the embryos with a tetraploid karyotype in a mosaic form (6.71 %), Klinefelter syndrome in a mosaic form (6.04 %), and a triploid karyotype in a mosaic form (6.04 %). A statistically significant increase in the incidence of stillbirths with mosaic tetraploid karyotype was found in 2020. The share of this pathology was 25. %. Currently, there is no reliable information on the effect of the SARS-CoV-2 virus on the embryonic genome. Nevertheless, it is known that members of the coronavirus family are responsible for serious complications during pregnancy - pregnancy fading, fetal growth retardation, premature birth, death of the mother or fetal death in the neonatal period. In this connection, we can hypothesize that mitotic disorders and, as a consequence, appearance of embryos with a tetraploid karyotype seem to be associated with a mild SARS-CoV-2 infection that occurs in pregnant women in an inconspicuous form.https://periodicals.karazin.ua/biology/article/view/18835karyotype, chromosomal abnormalities, chorionic villi, stillbirth, anembryonia, undeveloped pregnancy |
| spellingShingle | O. Feskov I. Feskova Ye. Zhylkova V. Rudenko O. Blazhko Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester Вісник Харківського Національного Університету Імені В.Н. Каразіна: Серія Біологія karyotype, chromosomal abnormalities, chorionic villi, stillbirth, anembryonia, undeveloped pregnancy |
| title | Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| title_full | Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| title_fullStr | Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| title_full_unstemmed | Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| title_short | Change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| title_sort | change in the karyotype of the embryo as a cause of spontaneous abortion in the first trimester |
| topic | karyotype, chromosomal abnormalities, chorionic villi, stillbirth, anembryonia, undeveloped pregnancy |
| url | https://periodicals.karazin.ua/biology/article/view/18835 |
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