Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets
In this study, the efficacy of the commercial modified live PRRSV-1 vaccine “Ingelvac PRRSFLEX<sup>®</sup> EU” was assessed in weaned piglets experimentally infected with PRRSV strain AUT15-33. Seventy-four weaned piglets were allocated to five groups. Vaccinated (groups 1, 2, and 5) and...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/6/934 |
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| author | Sophie Duerlinger Christian Knecht Spencer Sawyer Gyula Balka Marianne Zaruba Till Ruemenapf Christian Kraft Poul Henning Rathkjen Andrea Ladinig |
| author_facet | Sophie Duerlinger Christian Knecht Spencer Sawyer Gyula Balka Marianne Zaruba Till Ruemenapf Christian Kraft Poul Henning Rathkjen Andrea Ladinig |
| author_sort | Sophie Duerlinger |
| collection | DOAJ |
| description | In this study, the efficacy of the commercial modified live PRRSV-1 vaccine “Ingelvac PRRSFLEX<sup>®</sup> EU” was assessed in weaned piglets experimentally infected with PRRSV strain AUT15-33. Seventy-four weaned piglets were allocated to five groups. Vaccinated (groups 1, 2, and 5) and non-vaccinated piglets (groups 3 and 4), infected with either a low dose (10<sup>3</sup> TCID<sub>50</sub>/dose; groups 2 and 4) or a high dose (10<sup>5</sup> TCID<sub>50</sub>/dose; groups 1 and 3) of the virus, were compared regarding clinical signs, average daily weight gain (ADG), lung lesions, viral load in serum, oral swabs, and tissue samples. In comparison to vaccinated animals, coughing increased notably in the second week after challenge in non-vaccinated piglets. During the same time period, vaccinated, high-dose-infected piglets showed significantly higher ADG (<i>p</i> < 0.05) than non-vaccinated, high-dose-infected animals. All infected piglets reached approximately the same viremia levels, but vaccinated animals showed both a significantly reduced viral load in oral fluid (<i>p</i> < 0.05) and tissue samples and significantly reduced lung lesions (<i>p</i> < 0.05). In conclusion, vaccination was able to increase ADG, reduce the amount of viral shedding via oral fluids, and reduce the severity of lung lesions and the viral load in tissue samples under experimental conditions. |
| first_indexed | 2024-03-09T22:17:34Z |
| format | Article |
| id | doaj.art-bf59bb7e71f4409a968bb030bf392f73 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T22:17:34Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-bf59bb7e71f4409a968bb030bf392f732023-11-23T19:21:41ZengMDPI AGVaccines2076-393X2022-06-0110693410.3390/vaccines10060934Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned PigletsSophie Duerlinger0Christian Knecht1Spencer Sawyer2Gyula Balka3Marianne Zaruba4Till Ruemenapf5Christian Kraft6Poul Henning Rathkjen7Andrea Ladinig8University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaUniversity Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaUniversity Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaDepartment of Pathology, University of Veterinary Medicine, 1078 Budapest, HungaryInstitute of Virology, Department for Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department for Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaBoehringer Ingelheim Vetmedica GmbH, 55216 Ingelheim, GermanyBoehringer Ingelheim Animal Health Nordics, 2100 Copenhagen, DenmarkUniversity Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaIn this study, the efficacy of the commercial modified live PRRSV-1 vaccine “Ingelvac PRRSFLEX<sup>®</sup> EU” was assessed in weaned piglets experimentally infected with PRRSV strain AUT15-33. Seventy-four weaned piglets were allocated to five groups. Vaccinated (groups 1, 2, and 5) and non-vaccinated piglets (groups 3 and 4), infected with either a low dose (10<sup>3</sup> TCID<sub>50</sub>/dose; groups 2 and 4) or a high dose (10<sup>5</sup> TCID<sub>50</sub>/dose; groups 1 and 3) of the virus, were compared regarding clinical signs, average daily weight gain (ADG), lung lesions, viral load in serum, oral swabs, and tissue samples. In comparison to vaccinated animals, coughing increased notably in the second week after challenge in non-vaccinated piglets. During the same time period, vaccinated, high-dose-infected piglets showed significantly higher ADG (<i>p</i> < 0.05) than non-vaccinated, high-dose-infected animals. All infected piglets reached approximately the same viremia levels, but vaccinated animals showed both a significantly reduced viral load in oral fluid (<i>p</i> < 0.05) and tissue samples and significantly reduced lung lesions (<i>p</i> < 0.05). In conclusion, vaccination was able to increase ADG, reduce the amount of viral shedding via oral fluids, and reduce the severity of lung lesions and the viral load in tissue samples under experimental conditions.https://www.mdpi.com/2076-393X/10/6/934porcine reproductive and respiratory syndrome virus (PRRSV)PRRSV-1 AUT15-33respiratory modelweaned pigletschallenge modelmodified live virus vaccine |
| spellingShingle | Sophie Duerlinger Christian Knecht Spencer Sawyer Gyula Balka Marianne Zaruba Till Ruemenapf Christian Kraft Poul Henning Rathkjen Andrea Ladinig Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets Vaccines porcine reproductive and respiratory syndrome virus (PRRSV) PRRSV-1 AUT15-33 respiratory model weaned piglets challenge model modified live virus vaccine |
| title | Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets |
| title_full | Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets |
| title_fullStr | Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets |
| title_full_unstemmed | Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets |
| title_short | Efficacy of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Vaccine against Experimental Infection with PRRSV AUT15-33 in Weaned Piglets |
| title_sort | efficacy of a modified live porcine reproductive and respiratory syndrome virus 1 prrsv 1 vaccine against experimental infection with prrsv aut15 33 in weaned piglets |
| topic | porcine reproductive and respiratory syndrome virus (PRRSV) PRRSV-1 AUT15-33 respiratory model weaned piglets challenge model modified live virus vaccine |
| url | https://www.mdpi.com/2076-393X/10/6/934 |
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