The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress
Late embryogenesis abundant (LEA) proteins play important roles in regulating plant growth and responses to various abiotic stresses. In this research, a genome-wide survey was conducted to recognize the LEA genes in <i>Glycine max</i>. A total of 74 GmLEA was identified and classified i...
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2023-10-01
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| author | Binhui Guo Jianhua Zhang Chunhong Yang Lu Dong Heng Ye Babu Valliyodan Henry T. Nguyen Li Song |
| author_facet | Binhui Guo Jianhua Zhang Chunhong Yang Lu Dong Heng Ye Babu Valliyodan Henry T. Nguyen Li Song |
| author_sort | Binhui Guo |
| collection | DOAJ |
| description | Late embryogenesis abundant (LEA) proteins play important roles in regulating plant growth and responses to various abiotic stresses. In this research, a genome-wide survey was conducted to recognize the LEA genes in <i>Glycine max</i>. A total of 74 GmLEA was identified and classified into nine subfamilies based on their conserved domains and the phylogenetic analysis. Subcellular localization, the duplication of genes, gene structure, the conserved motif, and the prediction of cis-regulatory elements and tissue expression pattern were then conducted to characterize GmLEAs. The expression profile analysis indicated that the expression of several GmLEAs was a response to drought and salt stress. The co-expression-based gene network analysis suggested that soybean LEA proteins may exert regulatory effects through the metabolic pathways. We further explored GnLEA4_19 function in <i>Arabidopsis</i> and the results suggests that overexpressed GmLEA4_19 in <i>Arabidopsis</i> increased plant height under mild or serious drought stress. Moreover, the overexpressed GmLEA4_19 soybean also showed a drought tolerance phenotype. These results indicated that GmLEA4_19 plays an important role in the tolerance to drought and will contribute to the development of the soybean transgenic with enhanced drought tolerance and better yield. Taken together, this study provided insight for better understanding the biological roles of <i>LEA</i> genes in soybean. |
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| language | English |
| last_indexed | 2024-03-10T21:43:04Z |
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| spelling | doaj.art-bf5c656761a5493fb0d206668f767d732023-11-19T14:31:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124191483410.3390/ijms241914834The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought StressBinhui Guo0Jianhua Zhang1Chunhong Yang2Lu Dong3Heng Ye4Babu Valliyodan5Henry T. Nguyen6Li Song7Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaDivision of Plant Sciences, University of Missouri, Columbia, MO 65211, USADepartment of Agriculture and Environmental Sciences, Lincoln University, Jefferson City, MO 65101, USADivision of Plant Sciences, University of Missouri, Columbia, MO 65211, USAJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaLate embryogenesis abundant (LEA) proteins play important roles in regulating plant growth and responses to various abiotic stresses. In this research, a genome-wide survey was conducted to recognize the LEA genes in <i>Glycine max</i>. A total of 74 GmLEA was identified and classified into nine subfamilies based on their conserved domains and the phylogenetic analysis. Subcellular localization, the duplication of genes, gene structure, the conserved motif, and the prediction of cis-regulatory elements and tissue expression pattern were then conducted to characterize GmLEAs. The expression profile analysis indicated that the expression of several GmLEAs was a response to drought and salt stress. The co-expression-based gene network analysis suggested that soybean LEA proteins may exert regulatory effects through the metabolic pathways. We further explored GnLEA4_19 function in <i>Arabidopsis</i> and the results suggests that overexpressed GmLEA4_19 in <i>Arabidopsis</i> increased plant height under mild or serious drought stress. Moreover, the overexpressed GmLEA4_19 soybean also showed a drought tolerance phenotype. These results indicated that GmLEA4_19 plays an important role in the tolerance to drought and will contribute to the development of the soybean transgenic with enhanced drought tolerance and better yield. Taken together, this study provided insight for better understanding the biological roles of <i>LEA</i> genes in soybean.https://www.mdpi.com/1422-0067/24/19/14834<i>LEA</i> gene familysoybeangenome-wide identificationevolutionary analysisabiotic stress<i>GmLEA4_19</i> |
| spellingShingle | Binhui Guo Jianhua Zhang Chunhong Yang Lu Dong Heng Ye Babu Valliyodan Henry T. Nguyen Li Song The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress International Journal of Molecular Sciences <i>LEA</i> gene family soybean genome-wide identification evolutionary analysis abiotic stress <i>GmLEA4_19</i> |
| title | The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress |
| title_full | The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress |
| title_fullStr | The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress |
| title_full_unstemmed | The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress |
| title_short | The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress |
| title_sort | late embryogenesis abundant proteins in soybean identification expression analysis and the roles of gmlea4 19 in drought stress |
| topic | <i>LEA</i> gene family soybean genome-wide identification evolutionary analysis abiotic stress <i>GmLEA4_19</i> |
| url | https://www.mdpi.com/1422-0067/24/19/14834 |
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