Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma
<p>Abstract</p> <p>Background</p> <p>Atrial natriuretic peptide (ANP) and its receptor, NPRA, have been extensively studied in terms of cardiovascular effects. We have found that the ANP-NPRA signaling pathway is also involved in airway allergic inflammation and asthma....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-07-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/10/1/66 |
| _version_ | 1818117705490759680 |
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| author | Cormier Stephania A Giaimo Joseph D Ahlert Terry A Hellermann Gary Chen Dongqing Kong Xiaoyuan Xu Weidong Wang Xiaoqin Li Xu Lockey Richard F Mohapatra Subhra Mohapatra Shyam S |
| author_facet | Cormier Stephania A Giaimo Joseph D Ahlert Terry A Hellermann Gary Chen Dongqing Kong Xiaoyuan Xu Weidong Wang Xiaoqin Li Xu Lockey Richard F Mohapatra Subhra Mohapatra Shyam S |
| author_sort | Cormier Stephania A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Atrial natriuretic peptide (ANP) and its receptor, NPRA, have been extensively studied in terms of cardiovascular effects. We have found that the ANP-NPRA signaling pathway is also involved in airway allergic inflammation and asthma. ANP, a C-terminal peptide (amino acid 99–126) of pro-atrial natriuretic factor (proANF) and a recombinant peptide, NP73-102 (amino acid 73–102 of proANF) have been reported to induce bronchoprotective effects in a mouse model of allergic asthma. In this report, we evaluated the effects of vessel dilator (VD), another N-terminal natriuretic peptide covering amino acids 31–67 of proANF, on acute lung inflammation in a mouse model of allergic asthma.</p> <p>Methods</p> <p>A549 cells were transfected with pVD or the pVAX1 control plasmid and cells were collected 24 hrs after transfection to analyze the effect of VD on inactivation of the extracellular-signal regulated receptor kinase (ERK1/2) through western blot. Luciferase assay, western blot and RT-PCR were also performed to analyze the effect of VD on NPRA expression. For determination of VD's attenuation of lung inflammation, BALB/c mice were sensitized and challenged with ovalbumin and then treated intranasally with chitosan nanoparticles containing pVD. Parameters of airway inflammation, such as airway hyperreactivity, proinflammatory cytokine levels, eosinophil recruitment and lung histopathology were compared with control mice receiving nanoparticles containing pVAX1 control plasmid.</p> <p>Results</p> <p>pVD nanoparticles inactivated ERK1/2 and downregulated NPRA expression in vitro, and intranasal treatment with pVD nanoparticles protected mice from airway inflammation.</p> <p>Conclusion</p> <p>VD's modulation of airway inflammation may result from its inactivation of ERK1/2 and downregulation of NPRA expression. Chitosan nanoparticles containing pVD may be therapeutically effective in preventing allergic airway inflammation.</p> |
| first_indexed | 2024-12-11T04:42:39Z |
| format | Article |
| id | doaj.art-bf5e23ed874f4d5d9633f187aaff0292 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-11T04:42:39Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-bf5e23ed874f4d5d9633f187aaff02922022-12-22T01:20:35ZengBMCRespiratory Research1465-99212009-07-011016610.1186/1465-9921-10-66Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthmaCormier Stephania AGiaimo Joseph DAhlert Terry AHellermann GaryChen DongqingKong XiaoyuanXu WeidongWang XiaoqinLi XuLockey Richard FMohapatra SubhraMohapatra Shyam S<p>Abstract</p> <p>Background</p> <p>Atrial natriuretic peptide (ANP) and its receptor, NPRA, have been extensively studied in terms of cardiovascular effects. We have found that the ANP-NPRA signaling pathway is also involved in airway allergic inflammation and asthma. ANP, a C-terminal peptide (amino acid 99–126) of pro-atrial natriuretic factor (proANF) and a recombinant peptide, NP73-102 (amino acid 73–102 of proANF) have been reported to induce bronchoprotective effects in a mouse model of allergic asthma. In this report, we evaluated the effects of vessel dilator (VD), another N-terminal natriuretic peptide covering amino acids 31–67 of proANF, on acute lung inflammation in a mouse model of allergic asthma.</p> <p>Methods</p> <p>A549 cells were transfected with pVD or the pVAX1 control plasmid and cells were collected 24 hrs after transfection to analyze the effect of VD on inactivation of the extracellular-signal regulated receptor kinase (ERK1/2) through western blot. Luciferase assay, western blot and RT-PCR were also performed to analyze the effect of VD on NPRA expression. For determination of VD's attenuation of lung inflammation, BALB/c mice were sensitized and challenged with ovalbumin and then treated intranasally with chitosan nanoparticles containing pVD. Parameters of airway inflammation, such as airway hyperreactivity, proinflammatory cytokine levels, eosinophil recruitment and lung histopathology were compared with control mice receiving nanoparticles containing pVAX1 control plasmid.</p> <p>Results</p> <p>pVD nanoparticles inactivated ERK1/2 and downregulated NPRA expression in vitro, and intranasal treatment with pVD nanoparticles protected mice from airway inflammation.</p> <p>Conclusion</p> <p>VD's modulation of airway inflammation may result from its inactivation of ERK1/2 and downregulation of NPRA expression. Chitosan nanoparticles containing pVD may be therapeutically effective in preventing allergic airway inflammation.</p>http://respiratory-research.com/content/10/1/66 |
| spellingShingle | Cormier Stephania A Giaimo Joseph D Ahlert Terry A Hellermann Gary Chen Dongqing Kong Xiaoyuan Xu Weidong Wang Xiaoqin Li Xu Lockey Richard F Mohapatra Subhra Mohapatra Shyam S Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma Respiratory Research |
| title | Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| title_full | Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| title_fullStr | Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| title_full_unstemmed | Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| title_short | Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| title_sort | modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma |
| url | http://respiratory-research.com/content/10/1/66 |
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