Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model
Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861318301804 |
| _version_ | 1818193687614586880 |
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| author | Jiadai Liu Elena G. Rybakina Eelena A. Korneva Mami Noda |
| author_facet | Jiadai Liu Elena G. Rybakina Eelena A. Korneva Mami Noda |
| author_sort | Jiadai Liu |
| collection | DOAJ |
| description | Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options. Keywords: Pressure ulcer, Sodium salt of DNA, Oxidative damage, Inflammation, Mice |
| first_indexed | 2024-12-12T00:50:22Z |
| format | Article |
| id | doaj.art-bf5fc47450c444578e16270a565f7be8 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-12T00:50:22Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-bf5fc47450c444578e16270a565f7be82022-12-22T00:44:02ZengElsevierJournal of Pharmacological Sciences1347-86132018-10-011382123130Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse modelJiadai Liu0Elena G. Rybakina1Eelena A. Korneva2Mami Noda3Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of General Pathology and Pathophysiology, Federal State Budgetary Scientific Institution, Institute of Experimental Medicine, Str. Acad. Pavlova 12, Saint Petersburg 197376, Russia; Department of Pathology, Faculty of Medicine, Saint Petersburg State University, RussiaDepartment of General Pathology and Pathophysiology, Federal State Budgetary Scientific Institution, Institute of Experimental Medicine, Str. Acad. Pavlova 12, Saint Petersburg 197376, Russia; Department of Pathology, Faculty of Medicine, Saint Petersburg State University, RussiaLaboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Corresponding author. Fax: +81 92 642 6554.Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options. Keywords: Pressure ulcer, Sodium salt of DNA, Oxidative damage, Inflammation, Micehttp://www.sciencedirect.com/science/article/pii/S1347861318301804 |
| spellingShingle | Jiadai Liu Elena G. Rybakina Eelena A. Korneva Mami Noda Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model Journal of Pharmacological Sciences |
| title | Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model |
| title_full | Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model |
| title_fullStr | Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model |
| title_full_unstemmed | Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model |
| title_short | Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model |
| title_sort | effects of derinat on ischemia reperfusion induced pressure ulcer mouse model |
| url | http://www.sciencedirect.com/science/article/pii/S1347861318301804 |
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