Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276
Introduction. Up-to-date data and full characterization of circulating ASFV isolates play a crucial role in virus eradication and control in endemic regions and countries. The aim of the study was to evaluate and characterize the molecular and biological properties of the ASFV isolate ASF/Tatarst...
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Central Research Institute for Epidemiology
2023-09-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/13151/798 |
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| author | Andrey R. Shotin Roman S. Chernyshev Elizaveta O. Morozova Alexey S. Igolkin Konstantin N. Gruzdev Ivan S. Kolbin Ivan A. Lavrentiev Ali Mazloum |
| author_facet | Andrey R. Shotin Roman S. Chernyshev Elizaveta O. Morozova Alexey S. Igolkin Konstantin N. Gruzdev Ivan S. Kolbin Ivan A. Lavrentiev Ali Mazloum |
| author_sort | Andrey R. Shotin |
| collection | DOAJ |
| description | Introduction. Up-to-date data and full characterization of circulating ASFV isolates play a crucial role in virus eradication and control in endemic regions and countries.
The aim of the study was to evaluate and characterize the molecular and biological properties of the ASFV isolate ASF/Tatarstan 20/WB-12276, conduct phylogenetic analysis, and compare the results with isolates circulating in Europe and Asia.
Materials and methods. For bioassay, eight heads of the Large White pigs weighing 1520 kg/head were used. Detection of specific anti-ASFV antibodies by ELISA and immunoperoxidase method. Detection of ASFV genome was performed by qPCR. Isolation of ASF/Tatarstan 20/WB-12276 and determination of titer were performed in pig spleen cell culture. Sequencing was carried out by the Sanger method.
Results. The virus was characterized as highly virulent and capable of causing acute to subacute forms of ASF. Phylogenetic analysis revealed substitutions in the genome of the ASF/Tatarstan 20/WB-12276 isolate (IGR/I73R-I329L and I267L markers) that supported the clustering of the studied variant with isolates prevalent in most of Europe and Asia.
Conclusion. For the first time, the molecular and biological properties of the ASF/Tatarstan 20/WB-12276 virus isolate taken from a wild boar shot on the territory of the Republic of Tatarstan were studied and analyzed. |
| first_indexed | 2024-03-11T10:51:20Z |
| format | Article |
| id | doaj.art-bf602bd0f7534fc09213dd46c7c93540 |
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| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-11T10:51:20Z |
| publishDate | 2023-09-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-bf602bd0f7534fc09213dd46c7c935402023-11-13T17:14:22ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972023-09-0168430231410.36233/0507-4088-1821177Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276Andrey R. Shotin0https://orcid.org/0000-0001-9884-1841Roman S. Chernyshev1https://orcid.org/0000-0003-3604-7161Elizaveta O. Morozova2https://orcid.org/0000-0002-0955-9586Alexey S. Igolkin3https://orcid.org/0000-0002-5438-8026Konstantin N. Gruzdev4https://orcid.org/0000-0003-3159-1969Ivan S. Kolbin5https://orcid.org/0000-0003-4692-1297Ivan A. Lavrentiev6https://orcid.org/0009-0003-0552-3812Ali Mazloum7https://orcid.org/0000-0002-5982-8393Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Federal Center for Animal Health (ARRIAH)Introduction. Up-to-date data and full characterization of circulating ASFV isolates play a crucial role in virus eradication and control in endemic regions and countries. The aim of the study was to evaluate and characterize the molecular and biological properties of the ASFV isolate ASF/Tatarstan 20/WB-12276, conduct phylogenetic analysis, and compare the results with isolates circulating in Europe and Asia. Materials and methods. For bioassay, eight heads of the Large White pigs weighing 1520 kg/head were used. Detection of specific anti-ASFV antibodies by ELISA and immunoperoxidase method. Detection of ASFV genome was performed by qPCR. Isolation of ASF/Tatarstan 20/WB-12276 and determination of titer were performed in pig spleen cell culture. Sequencing was carried out by the Sanger method. Results. The virus was characterized as highly virulent and capable of causing acute to subacute forms of ASF. Phylogenetic analysis revealed substitutions in the genome of the ASF/Tatarstan 20/WB-12276 isolate (IGR/I73R-I329L and I267L markers) that supported the clustering of the studied variant with isolates prevalent in most of Europe and Asia. Conclusion. For the first time, the molecular and biological properties of the ASF/Tatarstan 20/WB-12276 virus isolate taken from a wild boar shot on the territory of the Republic of Tatarstan were studied and analyzed.https://virusjour.crie.ru/jour/article/viewFile/13151/798african swine feverrepublic of tatarstanbiological samplebiological propertiesmolecular genetic analysisphylogenetic analysisdendrogram |
| spellingShingle | Andrey R. Shotin Roman S. Chernyshev Elizaveta O. Morozova Alexey S. Igolkin Konstantin N. Gruzdev Ivan S. Kolbin Ivan A. Lavrentiev Ali Mazloum Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 Вопросы вирусологии african swine fever republic of tatarstan biological sample biological properties molecular genetic analysis phylogenetic analysis dendrogram |
| title | Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 |
| title_full | Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 |
| title_fullStr | Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 |
| title_full_unstemmed | Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 |
| title_short | Molecular and biological properties of the African swine fever virus (Asfarviridae: <i>Asfivirus</i>) isolate ASF/Tatarstan 20/WB-12276 |
| title_sort | molecular and biological properties of the african swine fever virus asfarviridae i asfivirus i isolate asf tatarstan 20 wb 12276 |
| topic | african swine fever republic of tatarstan biological sample biological properties molecular genetic analysis phylogenetic analysis dendrogram |
| url | https://virusjour.crie.ru/jour/article/viewFile/13151/798 |
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