Impact of photosensitizers activation on intracellular trafficking and viscosity.
The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3874004?pdf=render |
| _version_ | 1828482990483701760 |
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| author | Kelly Aubertin Stéphanie Bonneau Amanda K A Silva Jean-Claude Bacri François Gallet Claire Wilhelm |
| author_facet | Kelly Aubertin Stéphanie Bonneau Amanda K A Silva Jean-Claude Bacri François Gallet Claire Wilhelm |
| author_sort | Kelly Aubertin |
| collection | DOAJ |
| description | The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations (cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact. |
| first_indexed | 2024-12-11T08:27:25Z |
| format | Article |
| id | doaj.art-bf62c6809a584d89a370c7874ccf815a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:27:25Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bf62c6809a584d89a370c7874ccf815a2022-12-22T01:14:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8485010.1371/journal.pone.0084850Impact of photosensitizers activation on intracellular trafficking and viscosity.Kelly AubertinStéphanie BonneauAmanda K A SilvaJean-Claude BacriFrançois GalletClaire WilhelmThe intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations (cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.http://europepmc.org/articles/PMC3874004?pdf=render |
| spellingShingle | Kelly Aubertin Stéphanie Bonneau Amanda K A Silva Jean-Claude Bacri François Gallet Claire Wilhelm Impact of photosensitizers activation on intracellular trafficking and viscosity. PLoS ONE |
| title | Impact of photosensitizers activation on intracellular trafficking and viscosity. |
| title_full | Impact of photosensitizers activation on intracellular trafficking and viscosity. |
| title_fullStr | Impact of photosensitizers activation on intracellular trafficking and viscosity. |
| title_full_unstemmed | Impact of photosensitizers activation on intracellular trafficking and viscosity. |
| title_short | Impact of photosensitizers activation on intracellular trafficking and viscosity. |
| title_sort | impact of photosensitizers activation on intracellular trafficking and viscosity |
| url | http://europepmc.org/articles/PMC3874004?pdf=render |
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