A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

Summary: 16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for fecal DNA isolation, library preparation for 16S rRNA gene sequencing, taxonomy assignation, and statistical...

Full description

Bibliographic Details
Main Authors:	Magalí Martí, Johanne E. Spreckels, Maria C. Jenmalm, Thomas Abrahamsson
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Health Sciences Clinical Protocol Sequencing Microbiology Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721003592

Similar Items

<i>Lactobacillus reuteri</i> Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
by: Johanne E. Spreckels, et al.
Published: (2021-04-01)

Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
by: Hidenori Matsui, et al.
Published: (2023-12-01)

Effect of Probiotic Lactobacillus plantarum Dad-13 on Metabolic Profiles and Gut Microbiota in Type 2 Diabetic Women: A Randomized Double-Blind Controlled Trial
by: Rustanti, Ninik, et al.
Published: (2022)

Protocol for microbiota analysis of a murine stroke model
by: Adam Sorbie, et al.
Published: (2023-03-01)

Gut microbiota transplantation for colonization of germ-free mice
by: Jocelyn M. Choo, et al.
Published: (2021-09-01)

Alteration of the gut microbiota after surgery in preterm infants with necrotizing enterocolitis
by: Huijia Lin, et al.
Published: (2023-01-01)

Longitudinal 16S rRNA gut microbiota data of infant triplets show partial susceptibility to host genetics
by: Ondina Palmeira, et al.
Published: (2022-03-01)

Protocol for the isolation, sequencing, and analysis of the gut phageome from human fecal samples
by: Shirley Bikel, et al.
Published: (2022-03-01)

Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants
by: Eirin Esaiassen, et al.
Published: (2018-11-01)

Protocol for bioelectrochemical enrichment, cultivation, and characterization of extreme electroactive microorganisms
by: Ramandeep Singh, et al.
Published: (2022-03-01)

Implementing the Golden Hour Protocol to Improve the Clinical Outcomes in Preterm Infants
by: Seong Hee Jeong, et al.
Published: (2022-02-01)

Protocol for oral transplantation of maternal fecal microbiota to newborn infants born by cesarean section
by: Otto Helve, et al.
Published: (2021-03-01)

Dynamics of the bacterial gut microbiota in preterm and term infants after intravenous amoxicillin/ceftazidime treatment
by: Romy D. Zwittink, et al.
Published: (2020-05-01)

Gut microbiota non-convergence and adaptations in sympatric Tibetan and Przewalski’s gazelles
by: Pengfei Song, et al.
Published: (2024-03-01)

Delineating the causal role of the gut microbiota in experimental colitis
by: Jeffery, R
Published: (2022)

Establishment and resilience of transplanted gut microbiota in aged mice
by: Ying Wang, et al.
Published: (2022-01-01)

Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants
by: Cristina Alcon-Giner, et al.
Published: (2017-11-01)

Cervicovaginal microbiota and metabolome predict preterm birth risk in an ethnically diverse cohort
by: Flavia Flaviani, et al.
Published: (2021-08-01)

Lactoferrin impact on gut microbiota in preterm infants with late-onset sepsis or necrotising enterocolitis: the MAGPIE mechanisms of action study
by: Nicholas Embleton, et al.
Published: (2021-09-01)

Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
by: Xiaoqing Li, et al.
Published: (2022-09-01)

The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota
by: Shen Jean Lim, et al.
Published: (2021-01-01)

Feeding intolerance alters the gut microbiota of preterm infants.
by: Zhenya Yuan, et al.
Published: (2019-01-01)

Establishment of murine gut microbiota in gnotobiotic mice
by: Jocelyn M. Choo, et al.
Published: (2021-02-01)

Gut Microbiota Diversity of Preterm Neonates Is Associated With Clostridioides Difficile Colonization
by: Jeanne Couturier, et al.
Published: (2022-07-01)

Colostrum oropharyngeal immunotherapy for very low birth weight preterm infants: protocol of an intervention study
by: Camilla da Cruz Martins, et al.
Published: (2020-08-01)

Characteristics of intestinal microbiota in preterm infants and the effects of probiotic supplementation on the microbiota
by: Sen Yang, et al.
Published: (2024-03-01)

Meta-analysis of the gut microbiota in predicting response to cancer immunotherapy in metastatic melanoma
by: Angelo Limeta, et al.
Published: (2020-12-01)

The impact of neonatal intensive care unit antibiotics on gut bacterial microbiota of preterm infants: a systematic review
by: Martin M. Mulinge, et al.
Published: (2023-07-01)

Prenatal and postnatal antibiotic exposure influences the gut microbiota of preterm infants in neonatal intensive care units
by: Zhi-Hui Zou, et al.
Published: (2018-03-01)

Role of gut microbiota in the postnatal thermoregulation of Brandt’s voles
by: Tingbei Bo, et al.
Published: (2023-09-01)

The gut microbiota of people with asthma influences lung inflammation in gnotobiotic mice
by: Naomi G. Wilson, et al.
Published: (2023-02-01)

The microbiota drives diurnal rhythms in tryptophan metabolism in the stressed gut
by: Cassandra E. Gheorghe, et al.
Published: (2024-04-01)

Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice
by: Claudia M. Di Gesù, et al.
Published: (2023-09-01)

Integration of SNP genotyping and 16S rRNA amplicon sequencing to identify heritable gut microbes in chickens
by: Jinxin Zhang, et al.
Published: (2023-03-01)

Impavido attenuates inflammation, reduces atherosclerosis, and alters gut microbiota in hyperlipidemic mice
by: C. Alicia Traughber, et al.
Published: (2023-04-01)

Bacteriophages playing nice: Lysogenic bacteriophage replication stable in the human gut microbiota
by: Steven G. Sutcliffe, et al.
Published: (2023-02-01)

Antibiotic-induced disturbances of the gut microbiota result in accelerated breast tumor growth
by: Alastair M. McKee, et al.
Published: (2021-09-01)

The effect of early probiotic exposure on the preterm infant gut microbiome development
by: Yan Hui, et al.
Published: (2021-01-01)

Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics
by: Lucien Barnes V, et al.
Published: (2023-09-01)

Protocol to acquire time series data on adverse reactions following vaccination using a smartphone or web-based platform
by: Yasuo Yamao, et al.
Published: (2023-06-01)