Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection.
GATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasom...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-09-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC6179307?pdf=render |
| _version_ | 1828499573414297600 |
|---|---|
| author | Abiola O Olaitan Alejandro Aballay |
| author_facet | Abiola O Olaitan Alejandro Aballay |
| author_sort | Abiola O Olaitan |
| collection | DOAJ |
| description | GATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasome subunit, as an ELT-2 binding partner that is required for the proper expression of genes required for both immunity against bacterial infections and recovery after infection. We found that the intact ATPase domain of RPT-6 is required for the interaction and that inhibition of rpt-6 affected the expression of ELT-2-controlled genes, preventing the appropriate immune response against Pseudomonas aeruginosa and recovery from infection by the pathogen. Further studies indicated that SKN-1, which is an Nrf transcription factor involved in the response to oxidative stress and infection, is activated by inhibition of rpt-6. Our results indicate that RPT-6 interacts with ELT-2 in vivo to control the expression of immune genes in a manner that is likely independent of the proteolytic activity of the proteasome. |
| first_indexed | 2024-12-11T13:26:57Z |
| format | Article |
| id | doaj.art-bf670212c99049c38e01faac434a0932 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T13:26:57Z |
| publishDate | 2018-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-bf670212c99049c38e01faac434a09322022-12-22T01:05:29ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042018-09-01149e100769310.1371/journal.pgen.1007693Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection.Abiola O OlaitanAlejandro AballayGATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasome subunit, as an ELT-2 binding partner that is required for the proper expression of genes required for both immunity against bacterial infections and recovery after infection. We found that the intact ATPase domain of RPT-6 is required for the interaction and that inhibition of rpt-6 affected the expression of ELT-2-controlled genes, preventing the appropriate immune response against Pseudomonas aeruginosa and recovery from infection by the pathogen. Further studies indicated that SKN-1, which is an Nrf transcription factor involved in the response to oxidative stress and infection, is activated by inhibition of rpt-6. Our results indicate that RPT-6 interacts with ELT-2 in vivo to control the expression of immune genes in a manner that is likely independent of the proteolytic activity of the proteasome.http://europepmc.org/articles/PMC6179307?pdf=render |
| spellingShingle | Abiola O Olaitan Alejandro Aballay Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. PLoS Genetics |
| title | Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. |
| title_full | Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. |
| title_fullStr | Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. |
| title_full_unstemmed | Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. |
| title_short | Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection. |
| title_sort | non proteolytic activity of 19s proteasome subunit rpt 6 regulates gata transcription during response to infection |
| url | http://europepmc.org/articles/PMC6179307?pdf=render |
| work_keys_str_mv | AT abiolaoolaitan nonproteolyticactivityof19sproteasomesubunitrpt6regulatesgatatranscriptionduringresponsetoinfection AT alejandroaballay nonproteolyticactivityof19sproteasomesubunitrpt6regulatesgatatranscriptionduringresponsetoinfection |